We narrowed to 39,956 results for: lat

1. pBabe-Sik-WT

   Plasmid

   #210722

   Purpose

   Construct for stable expression of mouse wild type Ptk6 (Sik) in mammalian cells.

   Depositor

   Angela Tyner

   Article

   Derry et al Mol Cell Biol. 2000 Aug;20(16):6114-26.

   Insert

   Protein tyrosine kinase 6 (Ptk6 Mouse)
   

   Expression

   Mammalian

   Available Since

   Jan. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-DUET-GST-eIF4G1-His6, eIF4E

   Plasmid

   #37232

   Depositor

   Jon Lorsch

   Article

   Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

   Inserts

   TIF4631 (TIF4631 Budding Yeast)
   CDC33 (CDC33 Budding Yeast)
   

   Tags

   GST and His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 4, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pGEX-4T1-Claspin-FL-wt

   Plasmid

   #31621

   Depositor

   Aziz Sancar

   Article

   Lindsey-Boltz et al J Biol Chem. 2009 Nov 27. 284(48):33107-

   Insert

   Claspin (CLSPN Human)
   

   Tags

   Flag, GST, and His

   Expression

   Bacterial

   Available Since

   Nov. 9, 2011

   Availability

   Academic Institutions and Nonprofits only

4. mRIP3 GFP D161N

   Plasmid

   #41383

   Depositor

   Francis Chan

   Article

   Cho et al Cell. 2009 Jun 12;137(6):1112-23.

   Insert

   RIP3 (Ripk3 Mouse)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Kinase Domain Mutant (D161N)

   Promoter

   CMV

   Available Since

   Dec. 4, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pFLEX-m-cyclin D1 -T286A mutant

   Plasmid

   #86792

   Purpose

   mammalian expression of cyclin D1 T286A mutant

   Depositor

   Martine Roussel, Charles Sherr

   Article

   Matsushime et al Cell. 1991 May 17;65(4):701-13.

   Insert

   cyclin D1 (Ccnd1 Mouse)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   T286A

   Available Since

   Feb. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pG23A

   Plasmid

   #102884

   Purpose

   Plasmid used for the C-terminal tagging of Cdc14 with mCherry and the auxin-inducible degron in yeast.

   Depositor

   Matthias Heinemann

   Article

   Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi

   Insert

   Cdc14/mCherry-AID(71-114)-NatMX (CDC14 Budding Yeast)
   

   Tags

   AID(71-114) and mCherry

   Expression

   Yeast

   Promoter

   Native promoter

   Available Since

   Jan. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. Flag-MCPIP1(1-555)

   Plasmid

   #90105

   Purpose

   Mammalian expression of Flag-MCPIP1(1-555)

   Depositor

   Mingui Fu

   Article

   Liang et al J Exp Med. 2010 Dec 20;207(13):2959-73.

   Insert

   MCPIP1 (ZC3H12A Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hSyn-Flex-mRuby3-2A

   Plasmid

   #203846

   Purpose

   Neuron specific expression of a Cre-dependent mRuby3

   Depositor

   Ivo Spiegel

   Article

   Cohen-Kashi Malina et al Neuron. 2021 Jul 7;109(13):2150-2164.e5.

   Insert

   mRuby3
   

   Use

   AAV

   Available Since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLentiCRISPRv2 sgSp1

   Plasmid

   #174907

   Purpose

   Second generation sgRNA against Sp1

   Depositor

   Jane Clifford

   Article

   Swift et al Cell Rep. 2021 Mar 16;34(11):108840. doi

   Insert

   Specificity Protein 1 (SP1 Human)
   

   Expression

   Mammalian

   Mutation

   Missense mutation to mutate PAM sequence

   Promoter

   EFS-NS

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. psiCHECK2-miR-34 MT

    Plasmid

    #78259

    Purpose

    psiCHECK2 dual luciferase reporter harboring a mutated miR-34 target element, cloned into the XhoI/NotI restriction sites, the 3'UTR of the Renilla Luciferase gene

    Depositor

    Joanne Weidhaas

    Article

    Salzman et al Nat Commun. 2016 Mar 21;7:10954. doi: 10

    Insert

    mutated miR-34 target site
    

    Use

    Luciferase; Microrna activity

    Expression

    Mammalian

    Mutation

    nucleotides 2-5 and 10-11 changed from CCGT to GG…

    Available Since

    June 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMRXIP-GFP-STX17TMΔC

    Plasmid

    #221737

    Purpose

    Stable expression of GFP-STX17TMΔC in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

    Insert

    STX17 (STX17 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-228 and 227-302

    Available Since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. HA-Cdk9 T186A

    Plasmid

    #28103

    Depositor

    Andrew Rice

    Article

    Wang et al J Biol Chem. 2008 Nov 28. 283(48):33578-

    Insert

    Cdk9 (CDK9 Human)
    

    Tags

    3x HA

    Expression

    Bacterial and Mammalian

    Mutation

    Contains T186A mutation in Cdk9

    Available Since

    March 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pXD70CT5-PxylR-CymR, Pveg(CuO)-LacZ

    Plasmid

    #191627

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5 (engineered from pCT5-bac2.0)-LacZ

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    lacZ
    

    Expression

    Bacterial

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. TF-bio-His

    Plasmid

    #52184

    Purpose

    Expresses full-length Serotransferrin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    TF (TF Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJZC42

    Plasmid

    #66564

    Purpose

    sgRNA + 1XPP7 with PCP-VP64 effector for mammalian cells, marked by mCherry

    Depositor

    Wendell Lim, Stanley Qi

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Inserts

    sgRNA + 1XPP7
    PCP-VP64 IRES mCherry
    

    Expression

    Mammalian

    Promoter

    CMV and U6

    Available Since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSilencer2.1-U6-4JC1RR

    Plasmid

    #190847

    Purpose

    For mammalian expression of 4JC1RR, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker.

    Depositor

    Gerald Hart

    Article

    Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.

    Insert

    4JC1RR (A Dual-Specificity aptamer targeting OGT and GFP)
    

    Use

    Affinity Reagent/ Antibody and Synthetic Biology

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 9, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pSilencer2.1-U6-NL1F50

    Plasmid

    #190831

    Purpose

    For mammalian expression of NL1F50, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a flexible linker.

    Depositor

    Gerald Hart

    Article

    Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.

    Insert

    NL1F50 (A Dual-Specificity aptamer targeting OGT and GFP)
    

    Use

    Affinity Reagent/ Antibody and Synthetic Biology

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 9, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. Flag-MCPIP1(delta ZF)

    Plasmid

    #90110

    Purpose

    Mammalian expression of Flag-MCPIP1(delta ZF)

    Depositor

    Mingui Fu

    Article

    Liang et al J Exp Med. 2010 Dec 20;207(13):2959-73.

    Insert

    MCPIP1 (ZC3H12A Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    K317G, S318G

    Promoter

    CMV

    Available Since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMJB1-RPS22B-Cl

    Plasmid

    #160431

    Purpose

    Expresses "cre-less" yeast RPS22B tagged with mCherry under the GAL1 promoter

    Depositor

    Gary Stormo

    Article

    Roy et al Commun Biol. 2020 Dec 11;3(1):761. doi:

    Insert

    RPS22B (RPS22B Budding Yeast)
    

    Tags

    mCherry

    Expression

    Yeast

    Mutation

    Removed introns, shuffled codons to change nucleo…

    Promoter

    GAL1 and S. cerevisiae GAL1

    Available Since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBbdCas9S(-10TC)_Psyn-sgRNA500

    Plasmid

    #149583

    Purpose

    parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    PflaB, PpQE30, Psyn

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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