We narrowed to 7,325 results for: 11
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Plasmid#84634PurposeEncodes negative-control C-terminal (substrate) fragment of bimolecular JNK activity reporter (bimJNKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorInsertJNKsub(TA)DD-YPet-NES
Tags6xHis, Nuclear export signal (NES), T7 tag (gene …ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailable SinceNov. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-blast-cfTuba_1
Plasmid#26717DepositorInsertdog Tuba RNAi (DNMBP C. familiaris (dog RNAi))
UseLentiviral and RNAiExpressionMammalianMutationDog Tuba RNAiAvailable SinceDec. 15, 2010AvailabilityAcademic Institutions and Nonprofits only -
pTW080
Plasmid#115928PurposeSGP insert for replicon MoCloDepositorInsertLvl0 -98/30 SGP NA (No Aptamer)
UseSynthetic BiologyAvailable SinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pClneoEGFPC RSF-1
Plasmid#66862PurposeExpress GFP-fused C.elegans RSF-1 in mammalian cellsDepositorInsertRSF-1 (rsf-1 Nematode)
TagsGFPExpressionMammalianMutationS18G, S229T, I403T and F563S mutations in RSF-1 c…PromoterCMVAvailable SinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
MTK0_012
Plasmid#123967PurposeEncodes the hROSA26 BxBI recognition site with Hygromycin resistance and mRuby2 landing pad with GFP dropout destination vector as a type 0 part to be used in the MTK systemDepositorInserthRosa26-BxBI landing pad
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-blast-cfTuba_2
Plasmid#26718DepositorInsertdog Tuba RNAi (DNMBP C. familiaris (dog RNAi))
UseLentiviral and RNAiExpressionMammalianMutationCanine Tuba_2 RNAiAvailable SinceDec. 15, 2010AvailabilityAcademic Institutions and Nonprofits only -
sfGFP-Cx43K258stop
Plasmid#69025PurposeExpresses rat Cx43 (Gja1 CDS) truncated at amino acid 258 and tagged with sfGFP (V206-version) on the N-terminus. CMV promoter.DepositorInsertCx43 (Gja1 Rat)
Tagssuper folder GFPExpressionMammalianMutationnon-monomerized sfGFP fused in frame to N-terminu…PromoterCMVAvailable SinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-2
Plasmid#172739PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-2; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-2
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFL His-SDS22
Plasmid#113512PurposeExpression of His-SDS22 in insect cells (Baculovirus system)DepositorAvailable SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Rab4aM-7
Plasmid#56441PurposeLocalization: Ras GTPase, Excitation: 488, Emission: 507DepositorInsertRab4a (RAB4A Human)
TagsEGFPExpressionMammalianMutationMutation removes NheI sitePromoterCMVAvailable SinceJan. 29, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCDNA3 Flag MKK3b(Glu, delete LXL)
Plasmid#50450Purposeactivates p38 in the signaling pathwayDepositorInsertMKK3b (MAP2K3 Human)
TagsFlagExpressionMammalianMutationdeleted L25-I27, changed Serine 218 to Glutamic A…PromoterCMVAvailable SinceJan. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
JPF0522
Plasmid#124013PurposeEncodes crippled pEF1a driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vectorDepositorInsertPB_pEF1ac-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ERKsub(TA)DD-Venus-NES
Plasmid#84631PurposeEncodes negative-control C-terminal (substrate) fragment of bimolecular ERK activity reporter (bimEKAR); cytosol targetedDepositorInsertERKsub(TA)DD-Venus-NES
Tags6xHis, Nuclear export signal (NES), T7 tag (gene …ExpressionMammalianMutationMutated target Thr residue to Ala.PromoterCMVAvailable SinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
JPF0454
Plasmid#124039PurposeEncodes pPGK1 driving expression of spCas9 P2A mAzamiGreen in a 2nd gen. Lenti Virus destination vectorDepositorInsertpHR_pPGK1-spCAS9-P2A-mAzamiGreen
ExpressionMammalianAvailable SinceFeb. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
fgfprom luc
Plasmid#69446Purposebasal plasmid containing minimal fgf4 promoter used for testing enhancer activity of DNAs inserted immediately upstream of the promoter.DepositorInsertminimal fgf4 promoter
UseLuciferaseExpressionMammalianAvailable SinceMay 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
MTK8b_009
Plasmid#123896PurposeEncodes the 3' 500 bp hThal5.10-2-hg18 locus homology arm as a type 8b part to be used in the MTK systemDepositorInsertLA_hThal5.10-2-hg18
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK234_027
Plasmid#123907PurposeEncodes the spCas9 sgRNA1 hThal5.10-2_hg18 (site 1) gRNA expression cassette as a type 234 part to be used in the MTK systemDepositorInsertsgRNA1 -hThal5.10-2_hg18
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCfB3038(XII-1 MarkerFree)
Plasmid#73278PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-1 (Chr XII: 795787..796720)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionYeastAvailable SinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB3040(XII-4 MarkerFree)
Plasmid#73280PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-4 (Chr XII:830227..831248)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionYeastAvailable SinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
MTK0_001
Plasmid#123924PurposeEncodes the spCas9 gRNA GFP dropout expression cassette with ConLS and ConRE connectors with Ampicillin resistance as a type 0 part to be used in the MTK systemDepositorInsertTU-sgRNA - L1/RE
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLP-mEos2-Cep123
Plasmid#72589PurposemEos2 photoconvertible fluorescent protein fused to the N-terminus of isoform 1 of Cep123 (Cep89/CCDC123)DepositorAvailable SinceApril 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
MTK0_049
Plasmid#123946PurposeEncodes the hThal5.10-2_hg18 landing pad destination vector as a type 0 part to be used in the MTK systemDepositorInserthThal5.10-2_hg18 landing pad destination vector
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
GST-AR-4-52
Plasmid#104199PurposeExpresses human AR-4-52 in in pGEX-4T-1 as GST fusion proteinDepositorAvailable SinceDec. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag-Trib2
Plasmid#131155PurposeMammalian expression of flag-tagged mouse TRIB2DepositorAvailable SinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCfB3037(XI-5 MarkerFree)
Plasmid#73277PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-5 (Chr XI: 11779..118967)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionYeastAvailable SinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTW088
Plasmid#115936Purpose3'UTR insert for replicon MoCloDepositorInsertLvl0 3'UTR No E1
UseSynthetic BiologyAvailable SinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTW094
Plasmid#115937Purpose3'UTR insert for replicon MoCloDepositorInsertLvl0 E1-3'UTR End
UseSynthetic BiologyAvailable SinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
AI-CN590
Plasmid#62596PurposeKCTD13 N-terminus disruption by homolgy-directed repairDepositorInsertCerulean-P2A-PuroR (KCTD13 Human)
UseTALEN; Homology-directed repair donorExpressionMammalianPromoterhEF1alphaAvailable SinceMarch 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTYB3-human PUM1-HD PUF531_Q867E-Q939E-C935S-Q1011E- C1007S-intein-CBD
Plasmid#73306PurposeExpresses mutant human PUM1-HDDepositorInserthuman PUM1 Pumilio homology domain (PUM1 Human)
TagsinteinExpressionBacterialMutationQ867E-Q939E-C935S-Q1011E-C1007SAvailable SinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
PatoG-OG241
Plasmid#72839PurposeInduced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the native atoDAEB promoter (Pato) from E. coli DH5A. OG241 backbone.DepositorInsertPatoG
ExpressionBacterialPromoterPatoGAvailable SinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only