We narrowed to 8,360 results for: construct

1. pM-mT-Sapphire

   Plasmid

   #228616

   Purpose

   Mammalian expression of mT-Sapphire and extra insert, for MuSIC barcode construction

   Depositor

   Marc Birtwistle

   Article

   Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

   Insert

   mT-Sapphire
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET28a-SdrG-B2-ELP-HIS-ybbR

   Plasmid

   #169134

   Purpose

   E. coli expression of SdrG N2, N3 and B2 domains containing ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements.

   Depositor

   Michael Nash

   Article

   Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi

   Insert

   Staphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G
   

   Use

   Tags

   3xELP linker, 6x Histag, and ybbr tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET28a-Fgß-FIVAR-ELP-HIS-ybbR

   Plasmid

   #169136

   Purpose

   E. coli expression of FIVAR domain containing Fgß tag, ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements.

   Depositor

   Michael Nash

   Article

   Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi

   Insert

   FIVAR (found in various architectures)
   

   Use

   Tags

   3xELP linker, 6x Histag, Fgβ peptide, and ybbr ta…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28a-SdrG-B1-ELP-HIS-ybbR

   Plasmid

   #169133

   Purpose

   E. coli expression of SdrG N2, N3 and B1 domains containing ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements.

   Depositor

   Michael Nash

   Article

   Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi

   Insert

   Staphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G
   

   Use

   Tags

   3xELP linker, 6x Histag, and ybbr tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. PTGFR-DuET

   Plasmid

   #213371

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   PTGFR (PTGFR Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   Sept. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. AAV-HGM (optimized)

   Plasmid

   #220988

   Purpose

   Adeno-associated viral vector to express HaloTag-GFP-mito fusion, a cytosolic-localized Halotag-GFP fusion construct

   Depositor

   Joshua Kritzer

   Article

   Deprey et al ACS Chem Biol. 2022 Jan 15. doi: 10.1021

   Insert

   HaloTag-GFP-mito
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. MRGPRX4-DuET

   Plasmid

   #213347

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   MRGPRX4 (MRGPRX4 Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. MRGPRX2-DuET

   Plasmid

   #213345

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   MRGPRX2 (MRGPRX2 Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. NylB-SCY_pET29b

   Plasmid

   #215415

   Purpose

   Expression construct for NylB-SCY gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   NylB-SCY
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   R187S/F264C/D370Y

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. M-NylC_pET28a

    Plasmid

    #215409

    Purpose

    Expression construct for M-NylC gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    M-NylC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. NPFF2-DuET

    Plasmid

    #213352

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    NPFF2 (NPFFR2 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. MLNR-DuET

    Plasmid

    #213339

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    MLNR (MLNR Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. NPBW2-DuET

    Plasmid

    #213351

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    NPBW2 (NPBWR2 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. SSTR4-DuET

    Plasmid

    #213379

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    SSTR4 (SSTR4 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PK1-DuET

    Plasmid

    #213366

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    PK1 (PROKR1 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PRRP-DuET

    Plasmid

    #213367

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    PRRP (PRLHR Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PTAFR-DuET

    Plasmid

    #213368

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    PTAFR (PTAFR Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. QRFP-DuET

    Plasmid

    #213372

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    QRFP (QRFP Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. P2RY8-DuET

    Plasmid

    #213365

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    P2RY8 (P2RY8 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MRGPRX3-DuET

    Plasmid

    #213346

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    MRGPRX3 (MRGPRX3 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. MTNR1B-DuET

    Plasmid

    #213349

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    MTNR1B (MTNR1B Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. NPS-DuET

    Plasmid

    #213353

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    NPS (NPS Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. MRGPRE-DuET

    Plasmid

    #213341

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    MRGPRE (MRGPRE Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. MRGPRG-DuET

    Plasmid

    #213343

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    MRGPRG (MRGPRG Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. SSTR5-DuET

    Plasmid

    #213380

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    SSTR5 (SSTR5 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. SUCNR1-DuET

    Plasmid

    #213381

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    SUCNR1 (SUCNR1 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. S1PR4-DuET

    Plasmid

    #213375

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    S1PR4 (S1PR4 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. PTGER1-DuET

    Plasmid

    #213370

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    PTGER1 (PTGER1 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET23a-H6-TEV-AncSZ

    Plasmid

    #214235

    Purpose

    Bacterial expression construct of AncSZ, an kinase domain engineered through ancestorial reconstruction of the kinase domains of both SYK and ZAP-70. For in vitro protein tyrosine phosphorylation

    Depositor

    Neel Shah

    Article

    Hobbs et al Protein Sci. 2022 Oct;31(10):e4411. doi:

    Insert

    Syk kinase (SYK Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Sequence gained through ancestral reconstruction …

    Promoter

    T7

    Available since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pBabe-Sik-YF

    Plasmid

    #210723

    Purpose

    Construct for stable expression of constitutively active mouse Ptk6 (Sik) in mammalian cells.

    Depositor

    Angela Tyner

    Article

    Derry et al Mol Cell Biol. 2000 Aug;20(16):6114-26.

    Insert

    Protein tyrosine kinase 6 (Ptk6 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Tyrosine 447 changed to Phenylalanine

    Promoter

    Available since

    Jan. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. 10X PRE TK luc

    Plasmid

    #206162

    Purpose

    Luciferase reporter construct containing 10 consensus PGR binding sites upstream of a minimal TK promoter, derived from Addgene #11350

    Depositor

    Renee van Amerongen

    Article

    Aarts et al J Mammary Gland Biol Neoplasia. 2023 Nov

    Insert

    10X PRE TK luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    min TK

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. 8X PRE TK luc

    Plasmid

    #206161

    Purpose

    Luciferase reporter construct containing 8 consensus PGR binding sites upstream of a minimal TK promoter, derived from Addgene #11350

    Depositor

    Renee van Amerongen

    Article

    Aarts et al J Mammary Gland Biol Neoplasia. 2023 Nov

    Insert

    8X PRE TK luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    min TK

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. 6X PRE TK luc

    Plasmid

    #206160

    Purpose

    Luciferase reporter construct containing 6 consensus PGR binding sites upstream of a minimal TK promoter, derived from Addgene #11350

    Depositor

    Renee van Amerongen

    Article

    Aarts et al J Mammary Gland Biol Neoplasia. 2023 Nov

    Insert

    6X PRE TK luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    min TK

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pL0_BC-pyr_comp_sig_pep

    Plasmid

    #202132

    Purpose

    Level 0 plasmid containing a kozak consensus sequence and a pyrenoid compartment signal peptide sequence with BC overhangs used to build level 1 constructs.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    Pyrenoid compartment signal peptide
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Sept. 6, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

35. pL0-EF-54730

    Plasmid

    #202163

    Purpose

    Level 0 plasmid containing a terminator sequence from gene 54730 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    54730 terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Sept. 6, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

36. pL0_AC-27877

    Plasmid

    #202110

    Purpose

    Level 0 plasmid containing the promoter from gene 27877 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    27877 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter sequence mutated to remove sapI sites fo…

    Promoter

    Available since

    Aug. 29, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

37. pL0_CE-OriT

    Plasmid

    #202150

    Purpose

    Level 0 plasmid containing an origin of transfer sequence from pPtPBR11 required for making plasmids mobilizable for conjugation with CE overhangs used to build level 1 constructs.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    Origin of transfer
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    OriT sequence mutated to remove sapI sites domest…

    Promoter

    Available since

    Aug. 25, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

38. pL0_AC-Fld

    Plasmid

    #202125

    Purpose

    Level 0 plasmid containing the promoter from gene 23658 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    Fld promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter sequence mutated to remove bsaI site for…

    Promoter

    Available since

    Aug. 24, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

39. pL0_AC-54730

    Plasmid

    #202109

    Purpose

    Level 0 plasmid containing the promoter from gene 54730 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    54730 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter sequence mutated to remove bsaI site for…

    Promoter

    Available since

    Aug. 23, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

40. pL0_AC-51183

    Plasmid

    #202108

    Purpose

    Level 0 plasmid containing the promoter from gene 51183 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    51183 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter sequence mutated to remove sapI sites fo…

    Promoter

    Available since

    Aug. 23, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

41. pL0_AC-GEF

    Plasmid

    #202107

    Purpose

    Level 0 plasmid containing the promoter from gene 41365 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    GEF promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter sequence mutated to remove sapI site for…

    Promoter

    Available since

    Aug. 23, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

42. pL0_AC-47740

    Plasmid

    #202106

    Purpose

    Level 0 plasmid containing the promoter from gene 47740 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    47740 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter sequence mutated to remove bsaI site for…

    Promoter

    Available since

    Aug. 23, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

43. pL0_AC-BCA

    Plasmid

    #202105

    Purpose

    Level 0 plasmid containing the promoter from gene 51305 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    BCA promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter sequence mutated to remove sapI sites fo…

    Promoter

    Available since

    Aug. 23, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

44. pL0_AC-epyC

    Plasmid

    #202104

    Purpose

    Level 0 plasmid containing the promoter from gene 41316 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    epyC promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter sequence mutated to remove bsaI sites fo…

    Promoter

    Available since

    Aug. 23, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

45. pT2/sh-FYN1-GFP_Seq_C

    Plasmid

    #201399

    Purpose

    Knockdown of FYN tyrosine kinase. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

    Depositor

    Maria Castro

    Article

    Comba et al Neuro Oncol. 2020 Jun 9;22(6):806-818. d

    Insert

    FYN
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. AP70_pU.CAG.Cas9-D10A-K848A-R1060A.rBGpA

    Plasmid

    #199254

    Purpose

    Expression construct encoding the high specificity SpCas9-KARA-D10A nickase

    Depositor

    Manuel Goncalves

    Article

    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

    Insert

    High specificity S. pyogenes Cas9-D10A-KARA nickase
    

    Use

    CRISPR

    Tags

    3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A K848A R1060A

    Promoter

    CAG promoter

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pBabe NGF-EGFR-FRB-GluGlu neo

    Plasmid

    #192293

    Purpose

    Retroviral expression vector for NGF-EGFR-FRB-GluGlu construct

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

    Insert

    NGF-EGFR-FRB-GluGlu (EGFR Human)
    

    Use

    Retroviral

    Tags

    GluGlu

    Expression

    Mutation

    Promoter

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pBabe NGF-Erbb2-2xFKBP-HA hygro

    Plasmid

    #192291

    Purpose

    Retroviral expression vector for NGF-Erbb2-2xFKBP-HA construct

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

    Insert

    NGF-Erbb2-2xFKBP-HA (Erbb2 Rat)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. AP76_pU.CAG.Cas9-D10A-K848A.rBGpA

    Plasmid

    #199253

    Purpose

    Expression construct encoding the high specificity SpCas9-KA-D10A nickase

    Depositor

    Manuel Goncalves

    Article

    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

    Insert

    High specificity S. pyogenes Cas9-D10A-KA nickase
    

    Use

    CRISPR

    Tags

    3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A K848A

    Promoter

    CAG promoter

    Available since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. GFP-RAPH1Δ536-587

    Plasmid

    #194865

    Purpose

    Express eGFP-RAPH1 lacking amino acids 536-587 in mammalian cells. This constructs does not localise to filopodia anymore.

    Depositor

    Guillaume Jacquemet

    Article

    Popovic et al J Cell Sci. 2023 Mar 1;136(5):jcs260574.

    Insert

    RAPH1 (RAPH1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    RAPH1 with amino acids 536-587 deleted

    Promoter

    CMV

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge