We narrowed to 8,199 results for: Gal

1. EF1a-EGFP-FMRP-bGH

   Plasmid

   #235265

   Purpose

   ComMAND EGFP-FMRP base gene

   Depositor

   Kate Galloway

   Article

   Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

   Insert

   EGFP-Fmr1 (Fmr1 Mouse)
   

   Use

   Synthetic Biology

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a (human EF1a)

   Available Since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX552-hsyn DIO GCaMP8f(with gRNA scaffold)

   Plasmid

   #199580

   Purpose

   Expresses GCaMP8f in a Cre-dependent manner

   Depositor

   Bryan Copits

   Article

   Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

   Insert

   His-DIO GCaMP8f
   

   Use

   AAV, CRISPR, and Cre/Lox

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   hsyn

   Available Since

   Oct. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRRL-VcnTS-E1015A-E1021A

   Plasmid

   #213411

   Purpose

   Vinculin tension sensor (VcnTS) with both directionally asymmetric, force strengthening (DAFS) variant point mutations (E1015A and E1021A), in lentiviral expression vector.

   Depositor

   Brenton Hoffman

   Article

   Chirasani et al Nat Commun. 2023 Dec 14;14(1):8300. doi:

   Insert

   VcnTS-E1015A-E1021A (VCL Synthetic, Chicken)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Mutated vinculin glutamic acid 1015 and 1021 to a…

   Promoter

   CMV

   Available Since

   Feb. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-β-catenin_S23A,T40A,T41A,T112A

   Plasmid

   #190824

   Purpose

   For mammalian expression of β-catenin with 4 O-GlcNAc sites mutated (S23A, T40A, T41A and T112A) and GFP-tag on its N-terminus.

   Depositor

   Gerald Hart

   Article

   Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.

   Insert

   β-catenin (CTNNB1 Chicken)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   S23A, T40A, T41A, T112A

   Promoter

   CMV

   Available Since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. Coexpressed Free PcPTS

   Plasmid

   #182483

   Purpose

   Yeast integrative plasmid for expressing ERG20 (GAL10 promoter) and patchoulol synthase from Pogostemon cablin (PcPTS; GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

   Depositor

   Claudia Vickers

   Article

   Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

   Inserts

   FPPS
   PTS
   

   Use

   Synthetic Biology; Metabolic engineering

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL10 and GAL7

   Available Since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. deltaVP1 + VP2C-MIOX

   Plasmid

   #166680

   Purpose

   Yeast integrative plasmid for expressing deltaVP1 (GAL1 promoter) and mouse myo-inositol oxygenase tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

   Depositor

   Claudia Vickers

   Article

   Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

   Inserts

   VP2C-MIOX
   Murine polyomavirus deltaVP1 (NLS deletion mutant)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1 and GAL10

   Available Since

   April 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. wtVP1 + VP2C-GFP

   Plasmid

   #166674

   Purpose

   Yeast integrative plasmid for expressing wild-type Murine polyomavirus VP1 (GAL1 promoter) and GFP tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

   Depositor

   Claudia Vickers

   Article

   Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

   Inserts

   VP2C-GFP
   Wild-type Murine polyomavirus VP1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1 and GAL10

   Available Since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pZS1[FruR-L,RbsR-L]

   Plasmid

   #60761

   Purpose

   Contains PIq driving expression TreR-L, and PIq driving expression of RbsR-L

   Depositor

   Matthew Bennett

   Article

   Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

   Inserts

   FruR-L
   RbsR-L
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   LacI/GalR repressor chimera. LacI DBD with FruR L…

   Promoter

   Available Since

   July 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pZS1[RbsR-L,TreR-L]

   Plasmid

   #60766

   Purpose

   Contains PIq driving expression RbsR-L, and PI driving expression of TreR-L.

   Depositor

   Matthew Bennett

   Article

   Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

   Inserts

   RbsR-L
   TreR-L
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   LacI/GalR repressor chimera. LacI DBD with RbsR L…

   Promoter

   Available Since

   April 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGP-CMV-jGCaMP7f

    Plasmid

    #104483

    Purpose

    Mammalian expression of ultrasensitive protein calcium sensor

    Depositor

    GENIE Project, Douglas Kim

    Article

    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

    Insert

    jGCaMP7f
    

    Use

    Tags

    T7 epitope, Xpress tag, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28b-zDjCas13d-His

    Plasmid

    #234481

    Purpose

    Plasmid for bacterial expression and purification of DjCas13d protein (zebrafish codon-optimized)

    Depositor

    Miguel Angel Moreno-Mateos

    Article

    Moreno-Sanchez et al Nat Commun. 2025 Mar 16;16(1):2591. doi:

    Insert

    DjCas13d
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pStreptagIIx2 Alfatag 3C InsR EABR

    Plasmid

    #234996

    Purpose

    For production of Extracellular Vesicles (EVs), with the Insulin Receptor Strep-tag II labeled on their surface

    Depositor

    Daniel Leahy, Monica Gonzalez-Magaldi

    Article

    Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

    Insert

    InsR-EABR (INSR Human)
    

    Use

    Tags

    2xStreptag-II, Alfatag, HRV 3C site

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV GFAP jGCaMP7b

    Plasmid

    #171118

    Purpose

    Astrocyte AAV-mediated expression of bright ultrasensitive protein calcium sensor under the GFAP promoter

    Depositor

    Thomas Oertner

    Article

    Lohr et al Front Cell Neurosci. 2021 Jun 11;15:6901

    Insert

    jGCaMP7b
    

    Use

    AAV

    Tags

    6 His Tag, T7 Tag, and Xpress Tag

    Expression

    Mammalian

    Mutation

    Promoter

    GFAP

    Available Since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-Beatrix

    Plasmid

    #167528

    Purpose

    Dual-color fluorescent reporter for the creation and detection of Cre-dependent tunable mosaics in floxed mouse lines and cells.

    Depositor

    Gian Michele Ratto

    Article

    Trovato et al Nat Commun. 2020 Dec 3;11(1):6194. doi:

    Insert

    Beatrix
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    April 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCKC764

    Plasmid

    #226625

    Purpose

    piggyBAC pCAG-hCas9-T2A-Unbiased3

    Depositor

    Chang Liu

    Article

    Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

    Inserts

    CAG promoter, SpCas9, T2A, Unbiased3 variant of TdT (DNTT S. pyogenes, Human, Chicken)
    EF1a promoter, blasticidin resistance
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and EF1a

    Available Since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCIG-FGFRdn

    Plasmid

    #80431

    Purpose

    Expression of a dominant-negative FGFR1 with a nuclear EGFP reporter in chick embryos

    Depositor

    Aixa Morales

    Article

    Martinez-Morales et al J Cell Biol. 2011 Aug 8;194(3):489-503.

    Insert

    Fibroblast growth factor receptor 1 (FGFR1 Chicken)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    contains aa 1–425

    Promoter

    beta-actin promoter

    Available Since

    Feb. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

17. NINJA TC

    Plasmid

    #159274

    Purpose

    Expresses the NINJA targeting construct (inducible neoantigen expression via cumulative Cre, rtTA, doxycycline and tamoxifen).

    Depositor

    Nikhil Joshi

    Article

    Damo et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

    Insert

    NINJA TC
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV-hSyn-Ribo-jGCaMP8s

    Plasmid

    #167572

    Purpose

    DEPRECATED: Please see Addgene item #169247 for an improved version of this plasmid. AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8s.

    Depositor

    Marianne Fyhn

    Article

    Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

    Has Service

    AAV1

    Insert

    Ribo-GCaMP8s
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available Since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcU6_3 MS2 sgRNA

    Plasmid

    #92394

    Purpose

    Chick-specific U6 sgRNA expression mini-vector, harbouring chick U6_3 pol III promoter, with tracrRNA scaffold containing stem loops allowing binding of bacteriophage MS2 coat protein MCP

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Development. 2018 Feb 23;145(4). pii: de

    Insert

    chick U6.3 promoter and gRNA cloning cassette including MS2 stem loops
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    chick U6.3

    Available Since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAV-hSyn-Ribo-jGCaMP8m

    Plasmid

    #167574

    Purpose

    DEPRECATED: Please see Addgene item #169248 for an improved version of this plasmid. AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8m.

    Depositor

    Marianne Fyhn

    Article

    Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

    Has Service

    AAV1

    Insert

    Ribo-GCaMP8m
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available Since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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