We narrowed to 11,800 results for: KIN

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GFP-PELP1

Plasmid

#65208

Purpose

Full length mammalian expression vector for hPELP1

Depositor

Ratna Vadlamudi

Article

Nagpal et al Mol Cancer Res. 2008 May;6(5):851-61. do

Insert

PELP1 (PELP1 Human)

Tags

AcGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 29, 2015

Availability

Academic Institutions and Nonprofits only
pcdna3 Flag Jip1b

Plasmid

#52123

Purpose

a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.

Depositor

Roger Davis

Article

Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.

Insert

JNK interacting protein-1b (JIP-1b) (Mapk8ip1 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

April 28, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-IP3KA

Plasmid

#32529

Depositor

Christophe Erneux

Article

Dewaste et al Biochem J. 2003 Aug 15;374(Pt 1):41-9.

Insert

IP3KA (ITPKA Human)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 4, 2012

Availability

Academic Institutions and Nonprofits only
plk1 T210A

Plasmid

#132965

Purpose

To study the processes involved in early mitotic events, mitotic progression, and control of re-entry after DNA damage

Depositor

Michael Yaffe

Article

Kishi et al Mol Cell Biol. 2009 Jun;29(11):3134-50.

Insert

polo-like kinase 1 T210A (PLK1 Human)

Expression

Mammalian

Mutation

T210A

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223-RIOK3

Plasmid

#23537

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

RIOK3 (RIOK3 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-STK38L

Plasmid

#23810

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

STK38L (STK38L Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
TRUPATH Triple Ga15

Plasmid

#196060

Purpose

Encodes a G alpha subunit (GNA15) with RLuc8, a G gamma subunit (GNG13) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

Depositor

Justin English

Article

Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

Inserts

GAlpha15-RLuc8 (GNA15 Human)
GGamma13-GFP2 (GNG13 Human)
GBeta3 (GNB3 Human)

Use

Luciferase

Tags

GFP2 and GSAG linker

Expression

Mammalian

Mutation

RLuc8 and flanking SGGGGS linkers have been inser…

Promoter

CMV

Available Since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223-CHKA

Plasmid

#23428

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CHKA (CHKA Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-CLK3

Plasmid

#23753

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CLK3 (CLK3 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pCDM4-GLY

Plasmid

#49808

Purpose

Encodes pgk, gapA, aceE, aceF, lpdA in pseudo-operon configuration.

Depositor

Mattheos Koffas

Article

Xu et al Nat Commun. 2013;4:1409. doi: 10.1038/nc

Inserts

phosphoglycerate kinase
glyceraldehyde-3-phosphate dehydrogenase
pyruvate dehydrogenase subunit E1
dihydrolipoamide acetyltransferase
dihydrolipoamide dehydrogenase

Expression

Bacterial

Promoter

T7

Available Since

Feb. 14, 2014

Availability

Academic Institutions and Nonprofits only

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1099-N-cadherin-AAA-YFP

Plasmid

#32237

Depositor

Kathleen Green

Article

Chen et al J Cell Biol. 2003 Nov 10;163(3):547-57.

Insert

N-cadherin (Cdh2 Mouse)

Tags

EYFP

Expression

Mammalian

Mutation

Glu–Glu–Asp (aa 780–782) ---> Ala–Ala–Ala

Promoter

CMV

Available Since

Oct. 26, 2011

Availability

Academic Institutions and Nonprofits only
pRK5 Fyn SH2:R176E

Plasmid

#16035

Depositor

Filippo Giancotti

Article

Mariotti et al J Cell Biol. 2001 Oct 29. 155(3):447-58.

Insert

c-Fyn (FYN Human)

Expression

Mammalian

Mutation

SH2 point mutant: R176E

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pFastBac1-His10FLAG

Plasmid

#157736

Purpose

empty pFastBac1 vector (polyhedrin promoter) adds enterokinase-removable N-terminal His10-tag + 3xFLAG tag; avoids unwanted residues in the final protein sequence by using the BseRI restriction sites

Depositor

Debra Hansen

Article

Olson et al Protein Expr Purif. 2021 May 7:105890. d

Type

Empty backbone

Tags

His10 + 3xFLAG, enterokinase-cleavable

Expression

Insect

Promoter

polyhedrin

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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Hec1 mCherry Plk1 T210D

Plasmid

#45205

Depositor

Michael Lampson

Article

Liu et al J Cell Biol. 2012 Aug 20;198(4):491-9. d

Insert

Hec1-targeted mCherry Plk1 T210D

Tags

mCherry

Expression

Mammalian

Mutation

Plk1 T210D mutation

Promoter

T7

Available Since

May 30, 2013

Availability

Academic Institutions and Nonprofits only

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pDONR223-CCRK

Plasmid

#23717

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CCRK (CDK20 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-TXK

Plasmid

#23886

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

TXK (TXK Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pET15b-His-3C-irisin

Plasmid

#122612

Purpose

Expresses in E. coli: human irisin with Nt histag and 3C protease site for cleavage

Depositor

Harold Erickson

Article

Albrecht et al Sci Rep. 2015 Mar 9;5:8889. doi: 10.1038

Insert

irisin (ectodomain of FNDC5) (Fndc5 Human, Bovine, Mouse, Rat)

Tags

Nt: histag-3C protease site

Expression

Bacterial

Promoter

T7 polymerase

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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HC078 (3xHA-Atg13)

Plasmid

#59544

Purpose

Expresses yeast Atg13 under the endogenous promoter with a 3xHA N-terminal tag

Depositor

Daniel Klionsky

Article

Cheong et al Mol Biol Cell. 2008 Feb;19(2):668-81. Ep

Insert

ATG13 (ATG13 Budding Yeast)

Tags

3xHA

Expression

Bacterial and Yeast

Mutation

AvrII site created after start codon

Promoter

ATG13

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only
PKC beta II dNPS

Plasmid

#16383

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC beta II (Prkcb Mouse)

Tags

HA

Expression

Mammalian

Mutation

N-terminal pseudosubstrate domain deletion: aa 30…

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
pMCB306-GFP-Rab12

Plasmid

#198471

Purpose

lentiviral expression of GFP-Rab12

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

GFP-Rab12 (RAB12 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

EF1A

Available Since

April 18, 2023

Availability

Academic Institutions and Nonprofits only

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