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pLenti-lxlplap

Plasmid

#45581

Depositor

Nirao Shah

Article

Yang et al Cell. 2013 May 9;153(4):896-909. doi: 10

Inserts

Green fluorescent protein
Placental alkaline phosphatase (ALPP Human)

Use

Lentiviral

Tags

Histone 2B

Expression

Mammalian

Promoter

Ubiquitin

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pCAGGS-Flag-hsDicer (E1564A)

Plasmid

#41587

Depositor

Phil Sharp

Article

Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

Insert

hsDicer (DICER1 Human)

Tags

Flag

Expression

Mammalian

Mutation

E1564A

Promoter

CMV

Available Since

Feb. 7, 2013

Availability

Academic Institutions and Nonprofits only
piGECI-NES-N1

Plasmid

#160422

Purpose

Expression of a near-infrared genetically encoded calcium indicator iGECI with a nuclear export signal sequence

Depositor

Vladislav Verkhusha

Article

Shemetov et al Nat Biotechnol. 2021 Mar;39(3):368-377.

Insert

iGECI-NES

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pCAGGS-Flag-hsDicer (D1320A)

Plasmid

#41586

Depositor

Phil Sharp

Article

Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

Insert

hsDicer (DICER1 Human)

Tags

Flag

Expression

Mammalian

Mutation

D1320A

Promoter

CMV

Available Since

Feb. 7, 2013

Availability

Academic Institutions and Nonprofits only
pAd FLAG HDAC5 WT

Plasmid

#32209

Depositor

Reuben Shaw

Article

Mihaylova et al Cell. 2011 May 13;145(4):607-21.

Insert

HDAC5 (HDAC5 Human)

Use

Adenoviral

Tags

Flag

Expression

Mammalian

Available Since

Dec. 21, 2011

Availability

Academic Institutions and Nonprofits only
mOrange2-Tubulin-6

Plasmid

#57974

Purpose

Localization: Microtubules, Excitation: 549, Emission: 565

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Tubulin (TUBA1B Human)

Tags

mOrange2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
pEF1a-2xSV40_NLS-CeNLuc

Plasmid

#135952

Purpose

Expresses the CeNLuc LumiFluor targeted to the nucleus in mammalian cells.

Depositor

Antonio Amelio

Article

Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

Insert

CeNLuc LumiFluor

Tags

2xSV40-NLS

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pMC_mNG2(11)_BSDminus_F0

Plasmid

#184162

Purpose

Donor cassette plasmid for high-throughput tagging, encoding an mNG2(11) synthetic exon in frame 0, as well as a blasticidin resistance gene for selection of genomic integrants.

Depositor

Ophir Shalem

Article

Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

Inserts

mNG2(11)
bsd

Use

CRISPR

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pEF1a-T2-CeNLuc

Plasmid

#135948

Purpose

Expresses the CeNLuc LumiFluor targeted to the mitochondira in mammalian cells.

Depositor

Antonio Amelio

Article

Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

Insert

CeNLuc LumiFluor

Tags

T2 mitochondrial localization sequence

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pCRII-TOPO CMV-cGFP-SV40 Poly(A) Sense

Plasmid

#46836

Purpose

Express cGFP transcript ending in a poly(A) tail [using the SV40 poly(A) signal]

Depositor

Phil Sharp

Article

Wilusz et al Genes Dev. 2012 Nov 1;26(21):2392-407. d

Insert

Coral Green Fluorescent Protein (cGFP)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only

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pLenti-NIrD-Mefv iso1

Plasmid

#92108

Purpose

inducible expression in eukaryotic cells

Depositor

Feng Shao

Article

Gao et al Proc Natl Acad Sci U S A. 2016 Aug 16;11

Insert

Mefv isoform1 (Mefv Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pHJ424

Plasmid

#20682

Depositor

Stephen Shaw

Article

Hao et al J Cell Biol. 2009 Feb 9. 184(3):451-62.

Insert

Ezrin (EZR Human)

Tags

GFP

Expression

Mammalian

Mutation

Changed Lys 253, 254, 262, 263 to Asn

Available Since

April 14, 2009

Availability

Academic Institutions and Nonprofits only
∆C' terminal-DPYSL2-pLJC2

Plasmid

#189867

Purpose

Expresses human DPYSL2 (CRMP2) lacking the C' terminus (491-572 aa), in mammalian cells

Depositor

Yoav Shaul

Article

Abu Rmaileh et al J Cell Biol. 2022 Jul 4;221(7). pii: 213

Insert

ΔC-DPYSL2

Use

Lentiviral

Tags

3X FLAG

Expression

Mammalian

Mutation

deleted the C'terminus of DPYSL2 (491-572 aa)

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pRG/III-hs-MBP-A2aTr316-H10

Plasmid

#121683

Purpose

Bacterial expression of adenosine A2a receptor fusion protein

Depositor

Reinhard Grisshammer, Richard Henderson

Article

Weiss et al Eur J Biochem. 2002 Jan;269(1):82-92.

Insert

adenosine A2a receptor (ADORA2A Human)

Tags

His10

Expression

Bacterial

Promoter

lac

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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eDrTrkA

Plasmid

#183996

Purpose

Optically controlled eDrTrkA expression in mammalian cells

Depositor

Dmitry Gerashchenko, Vladislav Verkhusha

Article

Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

Insert

eDrTrkA (NTRK1 Human)

Tags

Ig kappa secretory signal, mCherry, Deinococcus r…

Expression

Mammalian

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-H2-Ld

Plasmid

#135519

Purpose

Mammalian expression of myc-tagged H2-Ld

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

H2-Ld

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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FLAG HDAC5 S259A

Plasmid

#32214

Depositor

Reuben Shaw

Article

Mihaylova et al Cell. 2011 May 13;145(4):607-21.

Insert

HDAC5 (HDAC5 Human)

Tags

Flag

Expression

Mammalian

Mutation

Ser259Ala

Promoter

CMV

Available Since

Nov. 2, 2011

Availability

Academic Institutions and Nonprofits only
pLaNGo11-1

Plasmid

#126053

Purpose

A self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cell

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Inserts

chloroplastic FNR transit peptide
ccdB cassette (BsaI)
GFP11

Use

Binary

Tags

GFP1-10

Expression

Plant

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pBAD-BpBBS

Plasmid

#199467

Purpose

Arabinose-induced expression of His-tagged biliverdin-binding serpin of Boana punctata treefrog (BpBBS) in E. coli.

Depositor

Vladislav Verkhusha

Article

Manoilov et al J Mol Biol. 2022 Jan 30;434(2):167359. d

Insert

BpBBS

Tags

6xHis

Expression

Bacterial

Available Since

June 23, 2023

Availability

Academic Institutions and Nonprofits only

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EF1a_SPIC_P2A_Hygro_Barcode

Plasmid

#120484

Purpose

Barcoded lentiviral vector to express SPIC in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

SPIC (SPIC Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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