We narrowed to 26,293 results for: Vit

1. psiCHECK2-miR-34 MT

   Plasmid

   #78259

   Purpose

   psiCHECK2 dual luciferase reporter harboring a mutated miR-34 target element, cloned into the XhoI/NotI restriction sites, the 3'UTR of the Renilla Luciferase gene

   Depositor

   Joanne Weidhaas

   Article

   Salzman et al Nat Commun. 2016 Mar 21;7:10954. doi: 10

   Insert

   mutated miR-34 target site
   

   Use

   Luciferase; Microrna activity

   Expression

   Mammalian

   Mutation

   nucleotides 2-5 and 10-11 changed from CCGT to GG…

   Available Since

   June 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. KHBD00222

   Plasmid

   #34370

   Depositor

   Bart Deplancke

   Article

   Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

   Insert

   CG1832 (CG1832 Fly)
   

   Use

   Gateway donor vector

   Available Since

   Jan. 23, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA3.1(+)-ExRai-AKAR(T/A)

   Plasmid

   #118406

   Purpose

   Negative-control mutant for ExRai-AKAR biosensor.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   ExRai-AKAR(T/A)
   

   Tags

   6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…

   Expression

   Mammalian

   Mutation

   Contains Thr-to-Ala mutation in substrate sequenc…

   Promoter

   CMV

   Available Since

   April 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-LbCas12a-Plus-mCherry

   Plasmid

   #191230

   Purpose

   To express the LbCas12a nuclease variant "LbCas12a-Plus" that enhanced the editing activity and specificity

   Depositor

   Zhili Rong

   Article

   Huang et al BMC Biol. 2022 Apr 25;20(1):91. doi: 10.

   Insert

   LbCas12a-Plus-P2A-mCherry
   

   Use

   CRISPR

   Mutation

   D156R, R883K, R887A

   Available Since

   Aug. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-Lyn-sapphireAKAR

   Plasmid

   #118466

   Purpose

   cp-T-sapphire-based single-color PKA biosensor targeted to the plasma membrane.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   Lyn-sapphireAKAR
   

   Tags

   N-terminal targeting sequence from Lyn kinase

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA-TO-HA-SH3BP1 (RNAi-res)

   Plasmid

   #136645

   Purpose

   Expression of SH3BP1 in mammalian cells; resistant to siRNA targeting GAUGACAGCCACCCACUUC

   Depositor

   Karl Matter, Maria Balda

   Article

   Elbediwy et al J Cell Biol. 2012 Aug 20;198(4):677-93.

   Insert

   SH3BP1 (RNAi resistant) (SH3BP1 Human)
   

   Tags

   HA (MQDLPGNDNSTAG)

   Expression

   Mammalian

   Mutation

   GATGACAGCCACCCACTTC changed to AATGACAGCAACACACTTC

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1(+)-sapphireAKAR(T/A)

   Plasmid

   #118465

   Purpose

   Negative-control mutant for sapphireAKAR biosensor.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   sapphireAKAR(T/A)
   

   Tags

   6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…

   Expression

   Mammalian

   Mutation

   Contains Thr-to-Ala mutation in substrate sequenc…

   Promoter

   CMV

   Available Since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-Lyn-AktAR2

   Plasmid

   #175013

   Purpose

   Plasma membrane-targeted AktAR2.

   Depositor

   Jin Zhang

   Article

   Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

   Insert

   Lyn-mCherry-Akt-STOPS
   

   Tags

   N-terminal targeting sequence from Lyn kinase

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a-HsSENP3(307-574)

   Plasmid

   #71481

   Purpose

   Encodes the catalytic domain of human SENP3. Suitable for in vitro transcription/translation (T7).

   Depositor

   Jorge Eduardo Azevedo

   Article

   Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

   Insert

   SUMO1/sentrin specific peptidase 3 (SENP3 Human)
   

   Tags

   Hexahistidine

   Expression

   Bacterial

   Mutation

   Deleted amino acids 1-306 (catalytic domain only)

   Promoter

   T7

   Available Since

   Dec. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA-TO-HA-SH3BP1 R312A (RNAi-res)

    Plasmid

    #136646

    Purpose

    Expression of SH3BP1R12A in mammalian cells; resistant to siRNA targeting GAUGACAGCCACCCACUUC

    Depositor

    Karl Matter, Maria Balda

    Article

    Elbediwy et al J Cell Biol. 2012 Aug 20;198(4):677-93.

    Insert

    SH3BP1R312A (RNAi resistant) (SH3BP1 Human)
    

    Tags

    HA (MQDLPGNDNSTAG)

    Expression

    Mammalian

    Mutation

    GATGACAGCCACCCACTTC changed to AATGACAGCAACACACTT…

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-RAB-AKARev(T/A)

    Plasmid

    #118479

    Purpose

    Negative-control mutant for RAB-AKARev biosensor.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    RAB-AKARev(T/A)
    

    Tags

    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Contains Thr-to-Ala mutation in substrate sequenc…

    Promoter

    CMV

    Available Since

    Dec. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-ING5

    Plasmid

    #13290

    Depositor

    Curtis Harris

    Article

    Shiseki et al Cancer Res. 2003 May 15. 63(10):2373-8.

    Insert

    ING5 (ING5 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA3-blueCKAR(T/A)

    Plasmid

    #118473

    Purpose

    Negative-control mutant for blueCKAR biosensor.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    blueCKAR(T/A)
    

    Tags

    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Contains Thr-to-Ala mutation in substrate sequenc…

    Promoter

    CMV

    Available Since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. KHBD00233

    Plasmid

    #34377

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG7803 (z Fly)
    

    Use

    Gateway donor vector

    Available Since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pCDNA3 14 mut B-Myb

    Plasmid

    #25967

    Depositor

    Rob Lewis

    Article

    Johnson et al J Biol Chem. 2002 Feb 8. 277(6):4088-97.

    Insert

    B-Myb (Mybl2 Mouse)
    

    Tags

    Flag

    Expression

    Bacterial

    Mutation

    Changed threonines 267, 408, 443, 447 490, 497, 5…

    Available Since

    Sept. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

16. KHBD00322

    Plasmid

    #34447

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG8591 (CTCF Fly)
    

    Use

    Gateway donor vector

    Available Since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

17. KHBD00311

    Plasmid

    #34436

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG9786 (hb Fly)
    

    Use

    Gateway donor vector

    Available Since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

18. KHBD00308

    Plasmid

    #34433

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG5204 (CG5204 Fly)
    

    Use

    Gateway donor vector

    Available Since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pET28a-HsSENP5

    Plasmid

    #71468

    Purpose

    Encodes human full length SENP5 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).

    Depositor

    Jorge Eduardo Azevedo

    Article

    Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

    Insert

    SUMO1/sentrin specific peptidase 5 (SENP5 Human)
    

    Tags

    Hexahistidine

    Expression

    Bacterial

    Mutation

    Gene optimised for E. coli expression (Genscript)

    Promoter

    T7

    Available Since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pET-SOMA

    Plasmid

    #118935

    Purpose

    Used to express the SOMA FRET sensor in E. coli.

    Depositor

    Patrick Krysan

    Article

    Zaman et al Plant J. 2018 Nov 16. doi: 10.1111/tpj.1

    Insert

    SOMA FRET sensor for measuring MAPK activity in Arabidopsis thaliana
    

    Expression

    Bacterial

    Available Since

    Feb. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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