We narrowed to 15,859 results for: cas9
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Plasmid#207878PurposeAAV vector encoding EF1a core promoter-driven SaCas9 and two sgRNA cloning sites w/ the engineered Sa Guide scaffold variant (BbsI and PaqCI for sgRNA cloning).DepositorTypeEmpty backboneUseAAV and CRISPRExpressionMammalianAvailable sinceOct. 13, 2023AvailabilityAcademic Institutions and Nonprofits only
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pspCas9-3exo-Tetra-com-CLCN5-sp-g1
Plasmid#176236PurposePlasmid for expressing a fusion protein of spCas9 and the 3’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.DepositorInsertspCas9 and polymerase exo domain fusion (CLCN5 Human)
ExpressionBacterialAvailable sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pspCas9-5Exo-tetra-com-hCLCN5-sp-g1
Plasmid#176237PurposePlasmid for expressing a fusion protein of spCas9 and the 5’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.DepositorInsertspCas9 and polymerase exo domain fusion (CLCN5 Human)
ExpressionBacterialAvailable sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-pMecp2-dSaCas9-VP64-pU6-sgRNA
Plasmid#158972PurposeVector C encodes pAAV-pMecp2-dSaCas9-VP64-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR activator in neuronsDepositorInsertdSaCas9-VP64
UseAAV and CRISPRExpressionMammalianPromoterpMecp2Available sinceSept. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pspCas9-Exo-tetra-com-hCLCN5-sp-g1
Plasmid#176238PurposePlasmid for expressing a fusion protein of spCas9 and the 5’ and 3’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.DepositorInsertspCas9 and polymerase exo domain fusion (CLCN5 Human)
ExpressionBacterialAvailable sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJEP317-pAAV-U6SaCas9gRNA(SapI)-EFS-GFP- KASH-pA
Plasmid#113694PurposeU6 driven SaCas9 gRNA expression cassette without a gRNA. Followed by an EFS driven GFP-KASH in a separate reading frame. SapI can be used to clone in gRNAs.DepositorInsertsSaCas9 gRNA Cassete
GFP
UseAAV and CRISPRTagsKASHAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
HiFi dFE
Plasmid#190142PurposeExpresses HiFiCas9-T4pol (HiFi dFE)DepositorPublicationInsertHiFi Cas9-T4pol (HiFi dFE)
UseCRISPRTags3xFLAGExpressionMammalianMutationSpCas9 R691A mutant, Codon Optimized for mammalia…PromoterCBhAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2_PROX1_1+4
Plasmid#121177PurposeLentiviral construct for the expression of Cas9 protein and puromycin resistance from EFS promoter and two guide RNAs targeting for deletion PROX1 start codon sequence.DepositorInsertCas9-P2A-puro and U6 promoters driven expression of gRNAs
UseCRISPR and LentiviralTagsP2A-puroExpressionMammalianAvailable sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCE38
Plasmid#174432PurposeC. auris LEUpOUT HYG marker CAS9 expression construct. Use with pCE41 gRNA expression construct.DepositorInsertC. auris LEU2 1 of 2, pENO1, Cas9, HYG 1 of 2
UseCRISPRExpressionBacterialAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SMVP-Cas9N-U6-gRNA TdR
Plasmid#80940PurposeExpresses Cas9N in mammalian cells; expresses gRNA TdR for Ai9 cleavage.DepositorInsertCas9N
UseAAV, CRISPR, and Synthetic BiologyExpressionMammalianAvailable sinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSLPB2B-FKBP12_F36V-SpdCas9-KRAB-tagBFP-PGK-Blasticidin
Plasmid#187953PurposeFKBP12 (F36V mutant) degron-tagged dCas9-KRAB domain fused to tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFKBP12_F36V-SpdCas9-KRAB-tagBFP
UseCRISPR and Synthetic BiologyTagsGGSExpressionMammalianAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
CAPTURE1-1_pLVX-EF1a-BirA-P2A-FB-dCas9-IRES-zsGreen1
Plasmid#138417PurposeCAPTURE1.1 vector containing BirA-V5-His, P2A, FB-dCas9, IRES and zsGreen1DepositorInsertsBirA
dCas9
UseLentiviralTagsFLAG, Avi-tag and V5, HisPromoterEF1aAvailable sinceJuly 29, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PB-CAG-NLS-dCas9-HA-2XNLS-10xGCN4-NLS-P2A-scFv-NLS-P65-HSF1-Flag-WPRE-PolyA-PGK-EM7-NeoR-polyA
Plasmid#168290PurposeCRISPR-activator Suntag-P65-HSF1 (SPH)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdCas9, Suntag-P65-HSF1
ExpressionMammalianPromoterCAGAvailable sinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
TK4_Zim3-dCas9-2xMyc-NLS-T2A-mApple_EF1a-Hygro
Plasmid#254456PurposeTK4 PiggyBac plasmid for stable expression of Zim3-dCas9 during human pluripotent stem cell differentiationDepositorInsertsZim3-dCas9-2xMyc-NLS-T2A-mApple
hygroR
UsePiggybacTagsT2A-mAppleExpressionMammalianPromoterCAG and EF1aAvailable sinceApril 24, 2026AvailabilityAcademic Institutions and Nonprofits only -
pUC19-B2KB46
Plasmid#103086PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertB2KB46(Cas9 coding gene from Elusimicrobium minutum (strain Pei191))
UseCRISPR; Cloning vectorMutationhuman codon-optimizedAvailable sinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-K0XCK7
Plasmid#103118PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertK0XCK7(Cas9 coding gene from Barnesiella intestinihominis YIT 11860)
UseCRISPR; Cloning vectorMutationhuman codon-optimizedAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-H1DEI0
Plasmid#103113PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertH1DEI0(Cas9 coding gene from Odoribacter laneus YIT 12061)
UseCRISPR; Cloning vectorMutationhuman codon-optimizedAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
G1397 DddAtox-C–SaKKH-Cas9(D10A)
Plasmid#157840Purposeexpresses split DddAtox-Cas9 construct in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertG1397 DddAtox-C–SaKKH-Cas9(D10A)–UGI–SV40 NLS
UseCRISPRAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
px459-sgPNPLA3
Plasmid#255036PurposeUsed to generate PLNPLA3 knockout; targets Exon 3DepositorInsertCRISPR Cas9 guide for PNPLA3 exon 3
UseCRISPRExpressionMammalianPromoterU6Available sinceJune 18, 2026AvailabilityAcademic Institutions and Nonprofits only -
pgTS40
Plasmid#169630PurposepX330 derived vector for PCAG driven expression of SpCas9 and PU6 driven expression of guide RNA OGTS40 (5' GGGGCCACTAGGGACAGGAT 3') targeting position 55115755 of chromosome 19.DepositorInsertU6-driven gRNA expression and PCAG-driven SpCas9 expression
ExpressionMammalianPromoterU6 / PCAGAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only