We narrowed to 6,089 results for: nls

1. pC014 - LwCas13a-msfGFP

   Plasmid

   #91902

   Purpose

   Expresses active LwCas13a for mammalian RNA targeting

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

   Insert

   LwCas13a
   

   Use

   CRISPR

   Tags

   NLS and msfGFP-NLS-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Dec. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-LSSmOrange-CNA35

   Plasmid

   #61605

   Purpose

   bacterial expression of N-terminal fusion protein of CNA35 with LSSmOrange

   Depositor

   Maarten Merkx

   Article

   Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

   Insert

   LSSmOrange and CNA35
   

   Use

   Tags

   His tag

   Expression

   Mutation

   Promoter

   T7

   Available since

   March 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pH-nCas9-PBE

   Plasmid

   #98163

   Purpose

   Expresses CRISPR base editor in rice

   Depositor

   Caixia Gao

   Article

   Zong et al Nat Biotechnol. 2017 May;35(5):438-440.

   Insert

   NLS-APOBEC1-XTEN-nCas9-UGI-NLS
   

   Use

   Rice

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Ubi

   Available since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a-mTurquoise2-CNA35

   Plasmid

   #61602

   Purpose

   bacterial expression of N-terminal fusion protein of CNA35 with m-Turquoise-2

   Depositor

   Maarten Merkx

   Article

   Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

   Insert

   mTurqouise and CNA35
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-mAmetrine-CNA35

   Plasmid

   #61604

   Purpose

   bacterial expression of N-terminal fusion protein of CNA35 with mAmetrine

   Depositor

   Maarten Merkx

   Article

   Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

   Insert

   mAmetrine and CNA35
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSB-TRE-CRISPRoff-EF1A-TetOn

   Plasmid

   #203355

   Purpose

   Drug-inducible expression of CRISPRoff components needed for DNA methylation and gene repression with Sleeping Beauty backbone

   Depositor

   Jonathan Brunger

   Article

   Gil et al Bio Protoc. 2024 Jan 5;14(1):e4913. doi:

   Insert

   CRISPRoff-TetOn
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pnCas9-PBE

   Plasmid

   #98164

   Purpose

   Expresses CRISPR base editor in protoplasts

   Depositor

   Caixia Gao

   Article

   Zong et al Nat Biotechnol. 2017 May;35(5):438-440.

   Insert

   NLS-APOBEC1-XTEN-nCas9-UGI-NLS
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Ubi

   Available since

   July 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCR1278

   Plasmid

   #111121

   Purpose

   Expresses XRCC2_-_152373174.23-P1P2 sgRNA in pCR1068

   Depositor

   Jacob Corn

   Article

   Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

   Insert

   XRCC2 gRNA (XRCC2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCR1279

   Plasmid

   #111122

   Purpose

   Expresses XRCC2_+_152373143.23-P1P2 sgRNA in pCR1068

   Depositor

   Jacob Corn

   Article

   Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

   Insert

   XRCC2 gRNA (XRCC2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCR1280

    Plasmid

    #111123

    Purpose

    Expresses XRCC3_+_104181759.23-P1P2 sgRNA in pCR1068

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    XRCC3 gRNA (XRCC3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCR1281

    Plasmid

    #111124

    Purpose

    Expresses XRCC3_+_104181786.23-P1P2 sgRNA in pCR1068

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    XRCC3 gRNA (XRCC3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. CAG-SaCas9-WPRE

    Plasmid

    #89996

    Purpose

    Expresses high levels of WT SaCas9 for genome editing

    Depositor

    Timo Otonkoski

    Article

    Balboa et al Stem Cell Res. 2017 Jul;22:16-19. doi: 1

    Insert

    SaCas9 (NEWENTRY Staphylococcus aureus)
    

    Use

    Tags

    3xHA, Nucleoplasmin NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMIA22

    Plasmid

    #214448

    Purpose

    Expresses BxbI-BP-NLS

    Depositor

    Roger Foo

    Article

    Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

    Insert

    BxbI
    

    Use

    Tags

    BP-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. FUS_anion

    Plasmid

    #98656

    Purpose

    Expresses untagged FUS AA 1-163

    Depositor

    Nicolas Fawzi

    Article

    Burke et al Mol Cell. 2015 Oct 15;60(2):231-41. doi:

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCR1319

    Plasmid

    #111116

    Purpose

    Expresses UBE2T_+_202311076.23-P1P2 sgRNA in pCR1068

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    UBE2T gRNA (UBE2T Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3-nuc-Nb37

    Plasmid

    #205515

    Purpose

    Nuclear-targeted Nb37 nanobody that binds and stabilizes the active form of Gαs G-protein subunit.

    Depositor

    Jin Zhang

    Article

    Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

    Insert

    nuc-Nb37
    

    Use

    Tags

    Nuclear localization signal (NLS) and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pB-nCas9-PBE

    Plasmid

    #98160

    Purpose

    Expresses CRISPR base editor in maize

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2017 May;35(5):438-440.

    Insert

    NLS-APOBEC1-XTEN-nCas9-UGI-NLS
    

    Use

    Maize

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Ubi

    Available since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. DCIP

    Plasmid

    #193860

    Purpose

    Encodes SV40-mCherry-GFP1-10 in pDGB1-aR backbone for plant expression. Requires pSOUP for replication in agrobacterium.

    Depositor

    Markita Landry

    Article

    Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

    Insert

    NLS-mCherry-GFP1-10
    

    Use

    Tags

    SV40 NLS

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    Aug. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3 MEKK1 KD

    Plasmid

    #12180

    Depositor

    Gary Johnson

    Article

    Widmann et al Mol Cell Biol. 1998 Apr . 18(4):2416-29.

    Insert

    MEKK1 (Map3k1 Mouse)
    

    Use

    Tags

    His and Xpress

    Expression

    Mammalian

    Mutation

    Kinase dead: K1253M; V1467L

    Promoter

    Available since

    Sept. 22, 2006

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA3-nuc-Nb80

    Plasmid

    #205519

    Purpose

    Nuclear-targeted Nb80 nanobody that binds and stabilizes β2AR active conformation, blocking Gαs coupling.

    Depositor

    Jin Zhang

    Article

    Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

    Insert

    nuc-Nb80
    

    Use

    Tags

    Nuclear localization signal (NLS) and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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