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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 6,501 results for: nls

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Showing: 2681 - 2700 of 6501 results

  1. pAAV-hSyn-DIO-FLAG-hM4D(Gi)-P2A-ArgiNLS-AausFP1

    Plasmid
    #220609
    Purpose
    Neuron-specific, Cre-dependent co-expression of FLAG-tagged inhibitory hM4D(Gi) DREADD receptor, and a single-cell discriminating version of AausFP1 as a reporter.
    Depositor
    Sam Golden
    Publication
    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121
    Insert
    FLAG-hM4D(Gi)-P2A-ArgiNLS-AausFP1
    Use
    AAV and Cre/Lox
    Tags
    FLAG; ArgiNLS
    Expression
    Mammalian
    Promoter
    hSyn
    Available since
    Dec. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    220609-plasmid-map-sequence-id-441116

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  2. mTagRFP-PCNA-19-SV40NLS-4

    Plasmid
    #57994
    Purpose
    Localization: Nucleus, Excitation: 555, Emission: 584
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PCNA-19 SV40NLS (PCNA Human)
    Tags
    mTagRFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 4, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  3. EBFP2-PCNA-19-SV40NLS-4

    Plasmid
    #55250
    Purpose
    Localization: Nucleus, Excitation: 383, Emission: 448
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PCNA-19 SV40NLS (PCNA Human)
    Tags
    3xSV40 NLS and EBFP2
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    55250-plasmid-map-sequence-id-230171

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  5. BB3cH_pGAP_23*_pPFK300_Cas9

    Plasmid
    #104912
    Purpose
    hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on Hyg
    Depositor
    Brigitte Gasser
    Publication
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Expression
    Yeast
    Available since
    Jan. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    104912-plasmid-map-sequence-id-206066

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  12. pDR111_Bgal NLS

    Plasmid
    #188396
    Purpose
    Expresses beta-galactosidase via Phyper-spank which is optimized for Bacillus subtilis. There is a secretion peptide (PhoD) and nuclear localization signal (SV40) that is fused to beta-galactosidase.
    Depositor
    Christopher Contag
    Publication
    Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:
    Insert
    lacZ
    Tags
    PhoD secretion peptide and SV40 NLS
    Expression
    Bacterial
    Promoter
    Phyper spank
    Available since
    Sept. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    188396-plasmid-map-sequence-id-373478

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  13. pcDNA3-P4:gs:nls:CIBN

    Plasmid
    #135986
    Purpose
    Expresses the P4 coiled coil fused to the CIBN blue light-inducible domain in mammalian cells
    Depositor
    Roman Jerala
    Publication
    Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    P4 coiled-coil fused to the CIBN domain
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    135986-plasmid-map-sequence-id-269554

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  17. mCerulean-PCNA-19-SV40NLS-4

    Plasmid
    #55386
    Purpose
    Localization: Nucleus, Excitation: 433, Emission: 475
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PCNA-19 SV40NLS (PCNA Human)
    Tags
    3xSV40 NLS and mCerulean
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 10, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
Showing: 2681 - 2700 of 6501 results