We narrowed to 9,921 results for: pET

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pET-28b His6-TEV-SHP2-FL(R4A,R5A)

Plasmid

#245194

Purpose

Production of His-tagged full-length SHP2

Depositor

Neel Shah

Publication

van Vlimmeren et al bioRxiv [Preprint]. 2025 Jul 4:2025.07.0

Insert

SHP2 full-length

Tags

6xHis

Expression

Bacterial

Mutation

R4A,R5A

Available since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pET-NIC-His6-TEV-SHP2-PTP(Q510E)

Plasmid

#245218

Purpose

Production of His-tagged SHP2 PTP domain

Depositor

Neel Shah

Publication

van Vlimmeren et al bioRxiv [Preprint]. 2025 Jul 2:2025.06.3

Insert

SHP2 PTP domain

Tags

6xHis

Expression

Bacterial

Mutation

Q510E

Available since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pET-NIC-His6-TEV-SHP2-PTP(Q510K)

Plasmid

#245219

Purpose

Production of His-tagged SHP2 PTP domain

Depositor

Neel Shah

Publication

van Vlimmeren et al bioRxiv [Preprint]. 2025 Jul 2:2025.06.3

Insert

SHP2 PTP domain

Tags

6xHis

Expression

Bacterial

Mutation

Q510K

Available since

Sept. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pET-29_GSS-6xHis-SUMO-VidC-EGFP-FLAG

Plasmid

#241009

Purpose

VidC-EGFP construct that can be purified via His-tag and undergo N-terminal conjugation using the VEPL system.

Depositor

Cole DeForest

Publication

Gharios et al Bioconjug Chem. 2024 Jun 19;35(6):750-75

Insert

GSS-6xHis-SUMO-VidC-EGFP-FLAG

Tags

FLAG-tag, His-tag, and SUMO-tag

Expression

Bacterial

Promoter

T7

Available since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pET-29_GSS-6xHis-SUMO-VidC-mCherry-FLAG

Plasmid

#241010

Purpose

VidC-mCherry construct that can be purified via His-tag and undergo N-terminal conjugation using the VEPL system.

Depositor

Cole DeForest

Publication

Gharios et al Bioconjug Chem. 2024 Jun 19;35(6):750-75

Insert

GSS-6xHis-SUMO-VidC-mCherry-FLAG

Tags

FLAG-tag, His-tag, and SUMO-tag

Expression

Bacterial

Promoter

T7

Available since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pET-21_GSS-6xHis-SUMO-VidC-bla-FLAG

Plasmid

#241012

Purpose

VidC-bla construct that can be purified via His-tag and undergo N-terminal conjugation using the VEPL system.

Depositor

Cole DeForest

Publication

Gharios et al Bioconjug Chem. 2024 Jun 19;35(6):750-75

Insert

GSS-6xHis-SUMO-VidC-bla-FLAG

Tags

FLAG-tag, His-tag, and SUMO-tag

Expression

Bacterial

Promoter

T7

Available since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pET-21_GSS-6xHis-SUMO-VidC-EGF-FLAG

Plasmid

#241011

Purpose

VidC-EGF construct that can be purified via His-tag and undergo N-terminal conjugation using the VEPL system.

Depositor

Cole DeForest

Publication

Gharios et al Bioconjug Chem. 2024 Jun 19;35(6):750-75

Insert

GSS-6xHis-SUMO-VidC-EGF-FLAG

Tags

FLAG-tag, His-tag, and SUMO-tag

Expression

Bacterial

Promoter

T7

Available since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pSR82: pET-SUMO-USP21 (S93A/S113A/S115A)

Plasmid

#240190

Purpose

Bacterial expression for USP21 (S93A/S113A/S115A)

Depositor

Cynthia Wolberger

Insert

USP21 (USP21 Human)

Tags

6x-His-SUMO

Expression

Bacterial

Mutation

Changed Serine 93, 113, and 115 to Alanine

Available since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET-GST-TNRC6A-(935-984)-NES-mut

Plasmid

#42051

Depositor

Kumiko Ui-Tei

Publication

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

TNRC6A (TNRC6A Human)

Tags

GST and His

Expression

Bacterial

Mutation

contain aa935-984, F951A, F955A, I958A, F960A

Available since

April 10, 2013

Availability

Academic Institutions and Nonprofits only

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pMW76: pET-His-MBP-SUMO-R2Bm-33XTEN- SpCas9(H840A)-SII

Plasmid

#209292

Purpose

Expresses R2Bm-Cas9(H840A) fusion in E. coli

Depositor

Feng Zhang

Publication

Wilkinson et al Science. 2023 Apr 21;380(6642):301-308.

Insert

R2

Tags

His, MBP, SpCas9(H840A), StrepII, and bdSUMO

Expression

Bacterial

Promoter

T7

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pET28(a)-p2c-Cas9

Plasmid

#242759

Purpose

Expresses Cas9 nuclease with fused p2c targeting ligand for expression in E. coli

Depositor

Jason Rasgon

Publication

Chaverra-Rodriguez et al Nat Commun. 2018 Aug 1;9(1):3008. doi: 1

Insert

P2C-Cas9

Use

CRISPR

Expression

Bacterial

Promoter

lac

Available since

Sept. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a-HaloTag-CNA35

Plasmid

#215457

Purpose

Encodes the bacterial collagen-binding protein CNA35 with N-terminal HaloTag.

Depositor

Johanna Ivaska

Publication

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

CNA35

Tags

HaloTag

Expression

Bacterial

Mutation

Includes stop codon

Available since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pET28b(+)_rPD-L1nb

Plasmid

#226715

Purpose

Heterologous expression and purification of recombinant PD-L1 nanobody (rPD-L1nb)

Depositor

Gautam Dantas

Publication

Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

Insert

Recombinant PD-L1 nanobody

Tags

HA tag and Histidine tag

Expression

Bacterial

Promoter

T7

Available since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-HIV p24

Plasmid

#212195

Purpose

Bacterial expression plasmid for production of HIV p24 protein with a polyhistidine tag at the N-terminus

Depositor

Richard C. Willson

Insert

Chain A, Capsid protein p24, HIV type 1

Tags

Polyhistidine

Expression

Bacterial

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET23a-H6-TEV-cSrc

Plasmid

#214233

Purpose

Bacterial expression of the kinase domain of cSrc with a TEV-cleavable N-terminal 6xHis affinity tag; For in vitro tyrosine phosphorylation in peptides/proteins and for kinase specificity screenings

Depositor

Neel Shah

Publication

Shah et al Elife. 2018 Mar 16;7. pii: 35190. doi: 1

Insert

cSrc Kinase domain (SRC Human)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET28sumo-hRig-I DCARDS

Plasmid

#210828

Purpose

protein expression in ecoli

Depositor

Anna Pyle

Publication

Luo et al Cell. 2011 Oct 14;147(2):409-22. doi: 10

Insert

hRIG-I DCARDS (DDX58) with Caspase Recruitment domains deleted (RIGI Human)

Expression

Bacterial

Mutation

NA

Available since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pET28C-mCherry-DDX4LCD

Plasmid

#204408

Purpose

E. coli expression of DDX4 low-complexity domain (1-236) with an N-terminal mCherry tag

Depositor

Samie Jaffrey

Publication

Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

Insert

DDX4(1-236) (DDX4 Human)

Tags

6x-His; mCherry

Expression

Bacterial

Promoter

T7

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pET28_mouse-pro-IL1B

Plasmid

#183386

Purpose

Overexpression of catalytic domain of inflammatory caspase in bacteria

Depositor

Jonathan Kagan

Publication

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

pro-IL1B (Il1b Mouse)

Tags

His-TEV

Expression

Bacterial

Promoter

T7

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pET28b-miniR-DBCO

Plasmid

#181984

Purpose

Used to label proteins with the MiniE genetically encoded tag

Depositor

Kimberly Beatty

Publication

Doh et al Biochemistry. 2020 Aug 25;59(33):3051-30

Insert

MiniR-DBCO

Tags

DBCO-Tag, His-Tag

Expression

Bacterial

Available since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-E988K

Plasmid

#173945

Purpose

Bacterial expression of inactive PARP1 mutant (E988K attenuates catalytic and PAR-branching activity of PARP1)

Depositor

Joan Conaway

Publication

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-E988K (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

V762A, E988K

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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