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FLAG-Matr3 delZnF2

Plasmid

#32884

Depositor

Yossi Shiloh

Publication

Salton et al PLoS One. 2011;6(8):e23882. Epub 2011 Au

Insert

MATR3 Matrin3 (MATR3 Human)

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

deleted amino acids 793-833

Promoter

CMV

Available since

Jan. 6, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pEGFP-C1-6xHis

Plasmid

#87343

Purpose

To express EGFP-6xHis in mammalian cell

Depositor

Lei Lu

Publication

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

6xHis

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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His6-PRC1

Plasmid

#69111

Purpose

For bacterial expression of recombinant human PRC1 tagged with His.

Depositor

Stephen Royle

Publication

Nixon et al Elife. 2015 Jun 19;4. doi: 10.7554/eLife

Insert

Protein Regulator of Cytokinesis 1 (PRC1 Human)

Tags

His6

Expression

Bacterial

Available since

Sept. 24, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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CTV

Plasmid

#27803

Depositor

Steven Vogel

Publication

Koushik et al J Biomed Opt. 2008 May-Jun;13(3):031204.

Insert

Cerulean Traf Venus

Use

Fret standard

Expression

Mammalian

Available since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
pGM1202

Plasmid

#69615

Purpose

inducible gene expression in Streptomyces, C-terminal His-tag encoding sequence

Depositor

Günther Muth

Publication

Muth et al Appl Microbiol Biotechnol. 2018 Nov;102(

Inserts

aac
tsr
rep
sso
TipA promoter
dso

Available since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLV-tetO-Tfap2a

Plasmid

#70274

Purpose

Lentiviral plasmid for tetracycline/doxycycline dependent expression of murine Tfap2a

Depositor

Hubert Schorle

Publication

Kubaczka et al Cell Stem Cell. 2015 Sep 22. pii: S1934-

Insert

Tfap2a (Tfap2a Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

tetO

Available since

Nov. 20, 2015

Availability

Academic Institutions and Nonprofits only
plentiCMV-sp-HPV-TM(ICAM)

Plasmid

#196510

Purpose

Express HPV antigen epitope with ICAM transmembrane domain

Depositor

Howard Chang

Publication

Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

Insert

a HPV epitope with CD8a stalk linker and ICAM TM domain

Use

Lentiviral

Tags

3xflag tag

Expression

Mammalian

Promoter

CMV

Available since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-MCP-huADARE488QT490A

Plasmid

#159922

Purpose

Fusion of MS2 coat protein to Human ADAR2 E488QT490A, under Ef1a promoter, with WPRE. AKA 116v5

Depositor

Fei Chen

Publication

Rodriques et al Nat Biotechnol. 2020 Oct 19. pii: 10.103

Insert

Human ADAR2 E488QT490A (ADARB1 Human)

Use

AAV

Promoter

EF1a

Available since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pAd GFP HDAC5 WT

Plasmid

#32217

Depositor

Reuben Shaw

Publication

Mihaylova et al Cell. 2011 May 13;145(4):607-21.

Insert

HDAC5 (HDAC5 Human)

Use

Adenoviral

Tags

GFP

Expression

Mammalian

Available since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pDONR223-BRD4

Plasmid

#23455

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

BRD4 (BRD4 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
MyoD-pCLBabe

Plasmid

#20917

Depositor

Stephen Tapscott

Publication

Yang et al Genes Dev. 2009 Mar 15. 23(6):694-707.

Insert

MyoD (Myod1 Mouse)

Use

Retroviral

Expression

Mammalian

Available since

April 28, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles
pKE4-I-SceI-fabp10a:Xpt-β-cat, cryaa:Venus

Plasmid

#105127

Purpose

Used along with I-SceI enzyme to generate transgenic zebrafish expressing hepatocyte-specific Xenopus activated beta-catenin and lens-specific Venus fluorescent protein

Depositor

Kimberley Evason, Didier Stainier

Publication

Evason et al PLoS Genet. 2015 Jul 2;11(7):e1005305. d

Inserts

Zebrafish fabp10a promoter:Xenopus pt-beta-catenin coding sequence, polyA (fabp10a Frog)
Zebrafish cryaa promoter:Venus fluorescent protein coding sequence (cryaa Zebrafish)

Use

Zebrafish transgenesis

Promoter

cryaa (crystallin) and fabp10a

Available since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCyPet-FHA2-AuroraB Substrate-YPet-IRES-puro2b

Plasmid

#40731

Depositor

Daniel Gerlich

Publication

Wurzenberger et al J Cell Biol. 2012 Jul 23;198(2):173-83.

Insert

Aurora B FRET-sensor

Tags

CyPet and YPet

Expression

Bacterial and Mammalian

Promoter

CMV

Available since

Oct. 29, 2012

Availability

Academic Institutions and Nonprofits only

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pME-puro-SNAP-FLAG-CD59

Plasmid

#50374

Purpose

Express SNAP and FLAG-tagged human CD59, a GPI-AP in mammmalian cells

Depositor

Reika Watanabe

Publication

Castillon et al Mol Biol Cell. 2013 Jun;24(12):2021-33.

Insert

SNAP

Expression

Mammalian

Promoter

SR alpha

Available since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pDONR223-STK24

Plasmid

#23595

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

STK24 (STK24 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pBabe-puro-miR-10b sponge

Plasmid

#25043

Depositor

Bob Weinberg

Publication

Ma et al Nat Biotechnol. 2010 Apr . 28(4):341-7.

Insert

miR-10b sponge

Use

Retroviral

Expression

Mammalian

Available since

June 11, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pBS_nanorod_105b

Plasmid

#120347

Purpose

phagemid containing all components for assembly of a DNA origami nanorod (10-helix bundle)

Depositor

Hendrik Dietz

Publication

Praetorius et al Nature. 2017 Dec 6;552(7683):84-87. doi:

Insert

Staple sequences for a DNA origami nanorod

Expression

Bacterial

Promoter

none

Available since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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Traptavidin-E6

Plasmid

#59549

Purpose

pET21-Tre for expression of traptavidin (high stability mutant of streptavidin) bearing a glutamic acid tag for ion-exchange chromatography

Depositor

Mark Howarth

Publication

Fairhead et al J Am Chem Soc. 2014 Sep 3;136(35):12355-

Insert

Core Traptavidin-E6

Tags

E6 tag

Expression

Bacterial

Promoter

T7

Available since

Oct. 6, 2014

Availability

Academic Institutions and Nonprofits only
Epac-S-H188

Plasmid

#170349

Purpose

mT2Del_EPACdDEPCD_tdcp173Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

Depositor

Kees Jalink

Publication

Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do

Insert

mT2Del_EPACdDEPCD_tdcp173Ven(ST) (RAPGEF3 Human)

Expression

Mammalian

Mutation

Deletion of DEP domain, Catalytically dead T781A …

Promoter

CMV

Available since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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TRE-Lepr_FL-IRES-eGFP

Plasmid

#196635

Purpose

Doxycycline inducible expression of full length mouse Lepr with eGFP marker

Depositor

Elaine Fuchs

Publication

Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

Insert

Lepr (Lepr Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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