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pEGFP-N1-PARP1-E988K

Plasmid

#211580

Purpose

CMV driven PARP1-E988K -eGFP expressing construct with IRES-Neomycin selection

Depositor

Chris Lord

Article

Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

Insert

PARP1 (PARP1 Human)

Tags

eGFP

Expression

Mammalian

Mutation

Contains E988K mutation

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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SIRT1.1

Plasmid

#13735

Depositor

John Denu

Article

Hallows et al Proc Natl Acad Sci U S A. 2006 Jul 5. 10

Insert

SIRT1 (SIRT1 Human)

Tags

His

Expression

Bacterial

Available Since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only

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Z-lock cofilin

Plasmid

#141137

Purpose

controlling the actin-severing ability of cofilin with light

Depositor

Klaus Hahn

Article

Stone et al Nat Chem Biol. 2019 Dec;15(12):1183-1190

Insert

Zdk2, Cofilin, LOV2

Expression

Mammalian

Mutation

mCherry, Zdk2(132F), Cofilin S3A, LOV WT

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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VSV.G

Plasmid

#14888

Depositor

Tannishtha Reya

Article

Reya et al Nature. 2003 May 22. 423(6938):409-14.

Insert

VSV-G Envelope

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 28, 2009

Availability

Academic Institutions and Nonprofits only

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AAVS1-TALEN-R

Plasmid

#59026

Purpose

TALEN designed to target the human AAVS1/PPP1R12C locus

Depositor

Danwei Huangfu

Article

Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

Insert

AAVS1-TALEN-R

Use

AAV and TALEN

Tags

3xFlag and NLS

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 12, 2014

Availability

Academic Institutions and Nonprofits only

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pHAGE-EZH2

Plasmid

#116738

Purpose

Lentiviral expression of EZH2

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

EZH2 (EZH2 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pATT-Dest

Plasmid

#79770

Purpose

Staging plasmid for use in DIP transposition

Depositor

David Savage

Article

Nadler et al Nat Commun. 2016 Jul 29;7:12266. doi: 10

Type

Empty backbone

Use

Synthetic Biology; Staging plasmid for transposit…

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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SlugMyc_pcDNA3

Plasmid

#31698

Depositor

Paul Wade

Article

Kajita et al Mol Cell Biol. 2004 Sep . 24(17):7559-66

Insert

Slug (SNAI2 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2011

Availability

Academic Institutions and Nonprofits only

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pNJP

Plasmid

#115494

Purpose

Expression vector for NLS-iRFP-NLS, JNK KTR-mCherry, and mKO-MK2 in mammalian cells

Depositor

Kazuhiro Aoki

Article

Miura et al Cell Rep. 2018 Sep 4;24(10):2658-2668. d

Insert

NLS-iRFP-NLS-P2A-JNK KTR-mCherry-P2A-mKO-MK2 (MAPK14, MAPK8 Mouse, Synthetic, Human)

Tags

iRFP, mCherry, and mKO

Expression

Mammalian

Mutation

mCherry S227F, mCherry G229R, mKO V211G

Promoter

CAG

Available Since

Dec. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1 (-) + FLAG-NLS-MS2-eGFP

Plasmid

#86827

Purpose

Expresses a nuclear localized, FLAG-tagged MS2-GFP fusion protein in mammalian cells, used for purification or localization of tagged RNAs

Depositor

Carl Novina

Article

Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

Insert

FLAG-NLS-MS2-eGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2017

Availability

Academic Institutions and Nonprofits only