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lentiGuide-tagBFP

Plasmid

#217007

Purpose

lentiviral plasmid for gRNA cloning with selectable tagBFP expression

Depositor

Deepta Bhattacharya

Article

D'Souza et al PLoS One. 2022 Aug 24;17(8):e0261801. do

Type

Empty backbone

Use

Lentiviral

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Tornado-F30-Broccoli-aptNFkB#5

Plasmid

#124362

Purpose

Overexpression of bifunctional circular RNA (Broccoli fluorescence and NFkB binding)

Depositor

Samie Jaffrey

Article

Litke et al Nat Biotechnol. 2019 Apr 8. pii: 10.1038

Insert

Tornado-F30-Broccoli-aptNFkB#5

Expression

Mammalian

Promoter

U6+27

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-rtTA-IN

Plasmid

#60612

Purpose

Backbone for tet-inducible genes

Depositor

Austin Smith

Article

Takashima et al Cell. 2014 Sep 11;158(6):1254-69. doi: 1

Type

Empty backbone

Available Since

May 5, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-mCherry-CW3SL

Plasmid

#223662

Purpose

Expresses mCherry in a Cre-dependent manner

Depositor

Oscar Marin

Article

Selten et al Nature. 2025 Apr 30. doi: 10.1038/s41586

Insert

mCherry

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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CIp10 - γmGFP (γmGFP based)

Plasmid

#163119

Purpose

Expresses in Candida albicans

Depositor

Patrick Van Dijck

Article

Van Genechten et al Sci Rep. 2020 Apr 9;10(1):6158. doi: 10.

Insert

gamma mGFP

Use

Candida albicans expression

Available Since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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mRNA-CleanCap-mNeonGreen

Plasmid

#188248

Purpose

Template plasmid to transcribe mRNA encoding mNeonGreen

Depositor

Howard Chang

Article

Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

Insert

mNeonGreen

Expression

Mammalian

Promoter

T7

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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PlayBack-Ef1aKpn1

Plasmid

#203307

Purpose

For expression of eukaryotic genes of interest with Ef1a promoter with Kpn1 as a PlayBack Restriction Enzyme

Depositor

Aleksandar Necakov

Article

Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

Type

Empty backbone

Expression

Mammalian

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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MSCB-hDot1Lwt

Plasmid

#74173

Purpose

For packaging hDot1Lwt to retrovirus.

Depositor

Yi Zhang

Article

Okada et al Cell. 2005 Apr 22;121(2):167-78.

Insert

hDot1L (DOT1L Human)

Use

Retroviral

Tags

HA

Available Since

May 23, 2016

Availability

Academic Institutions and Nonprofits only

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pLV-CMV-EGFP-Hygro

Plasmid

#184593

Purpose

EGFP

Depositor

Robert Sobol

Article

Koczor et al Methods Mol Biol. 2023;2609:43-59. doi:

Insert

EGFP

Use

Lentiviral

Promoter

CMV

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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GalToxBFP

Plasmid

#68073

Purpose

mammalian expression of GC localized oxBFP

Depositor

Erik Snapp

Article

Costantini et al Nat Commun. 2015 Jul 9;6:7670. doi: 10.1

Insert

GalT signal anchor transmembrane domain

Tags

moxBFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only

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pReg-RamR

Plasmid

#190610

Purpose

Expresses the RamR transcription factor in E. coli

Depositor

Andrew Ellington

Article

d'Oelsnitz et al Nat Chem Biol. 2022 Jul 7. pii: 10.1038/

Insert

RamR

Expression

Bacterial

Available Since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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MCP-M-MLV-RT

Plasmid

#181799

Purpose

MCP-M-MLV-RT (MS2-N55K-MMLVRT)

Depositor

Erik Sontheimer

Article

Liu et al Nat Biotechnol. 2022 Apr 4. pii: 10.1038

Insert

MCP-M-MLV-RT

Expression

Mammalian

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pOsU3

Plasmid

#170132

Purpose

For sgRNA expression in monocotyledons protoplasts

Depositor

Caixia Gao

Article

Lin et al Nat Biotechnol. 2021 Mar 25. pii: 10.103

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pT-CA-GFP-IRES-mLumin

Plasmid

#32749

Depositor

Jeanne Hardy

Article

Nicholls et al J Biol Chem. 2011 Jul 15;286(28):24977-8

Inserts

CA-GFP
mLumin

Expression

Mammalian

Mutation

S158A and S65T

Promoter

CMV

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pJH23

Plasmid

#159314

Purpose

Punc-25 GFP::SYD-2

Depositor

Mei Zhen

Article

Yeh et al J Neurosci. 2005 Apr 13;25(15):3833-41.

Insert

syd-2 (syd-2 Nematode)

Tags

GFP

Expression

Worm

Promoter

Punc-25

Available Since

Sept. 22, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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3xFLAG-NanoLuc

Plasmid

#189975

Purpose

Part 3-4-5 plasmid to introduce 3xFLAG-NanoLuc into circRNAs

Depositor

Howard Chang

Article

Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

Insert

NanoLuc

Use

Cloning

Tags

3xFLAG

Promoter

N/A

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pDAI-SceI

Plasmid

#32061

Purpose

Construction of unmarked gene deletions. Broad host range replicative plasmid expressing the I-SceI homing endonuclease

Depositor

Miguel Valvano

Article

Flannagan et al Environ Microbiol. 2008 Jun;10(6):1652-6

Insert

I-SceI nuclease

Expression

Bacterial

Promoter

Pdhfr

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pMKV059

Plasmid

#133314

Purpose

BeYDV viral replicon on T-DNA backbone expressing Firefly Luc+ and IPT

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Luc+, IPT

Expression

Plant

Promoter

AtUbi10, 35S

Available Since

Jan. 29, 2020

Availability

Academic Institutions and Nonprofits only

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CIp10 - mScarlet-I IDT

Plasmid

#163120

Purpose

Expresses in Candida albicans

Depositor

Patrick Van Dijck

Article

Van Genechten et al Sci Rep. 2020 Apr 9;10(1):6158. doi: 10.

Insert

mScarlet-I IDT

Use

Candida albicans expression

Available Since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pSaI14

Plasmid

#8614

Depositor

Robert Hartley

Article

Krajcikova et al J Bacteriol 1998 Mar;180(6):1582-5.

Insert

Ribonuclease inhibitor SaI14

Expression

Bacterial

Mutation

N terminal threonine changed to alanine

Available Since

Jan. 17, 2007

Availability

Academic Institutions and Nonprofits only