We narrowed to 3,002 results for: ALC

Showing: 2721 - 2740 of 3002 results

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pSG5-FLAG-CaMKKbeta rat FL

Plasmid

#32449

Depositor

Anthony Means

Article

Anderson et al Cell Metab. 2008 May;7(5):377-88.

Insert

Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)

Tags

FLAG

Expression

Mammalian

Mutation

S3L, G96R, and P108L

Promoter

SV40

Available Since

Feb. 1, 2012

Availability

Academic Institutions and Nonprofits only
Ca-LiPPI-CLAPon1.0 (APEX)

Plasmid

#213408

Purpose

Light-gated peroxidase (APEX) reporter for detecting calcium-dependent CaM and MK2 interaction (component 1)

Depositor

Wenjing Wang

Article

Zhou et al J Am Chem Soc. 2022 Dec 21;144(50):22933

Insert

MK2-E5-truncated hlov-TEVcs-APEX(201-250)-K5-HA-HA-APEX(2-200)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-eGFP-TAX1BP1 SKICH (1-140D)

Plasmid

#175768

Purpose

Lentiviral vector expressing N-terminally tagged eGFP-TAX1BP1 SKICH (1-140D)

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

TAX1BP1 (TAX1BP1 Human)

Use

Lentiviral

Mutation

SKICH (1-140D)

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pMIEG3-NFAT2

Plasmid

#40351

Depositor

Alexander Dent

Article

Wang et al J Immunol. 2006 Jan 15;176(2):880-8.

Insert

NFAT2 (NFATC1 Mouse)

Use

Retroviral

Tags

IRES eGFP

Expression

Mammalian

Available Since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only
pSG5-HA-CaMKKbeta rat FL WT

Plasmid

#33321

Depositor

Anthony Means

Article

Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

Insert

Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)

Tags

HA

Expression

Mammalian

Mutation

S3V, G96R, and P108L

Promoter

SV40

Available Since

April 17, 2012

Availability

Academic Institutions and Nonprofits only
pCDNA-SCW

Plasmid

#190633

Purpose

Mammalian expression of genetically encoded calcium indicator SCW

Depositor

Robert Campbell

Article

Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

Insert

SCW

Expression

Mammalian

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pBAD-SCW

Plasmid

#190626

Purpose

Bacterial expression of genetically encoded calcium indicator SCW

Depositor

Robert Campbell

Article

Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

Insert

SCW

Expression

Bacterial

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCRII-Topo S100a10 in situ probe

Plasmid

#45603

Depositor

Jeffrey Macklis

Article

Arlotta et al Neuron. 2005 Jan 20;45(2):207-21.

Insert

s100a10 in situ probe (S100a10 Mouse)

Use

In situ

Mutation

fragment contains bp# 21-275 of BC025044

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
pET28a-His6-OAZ-GSY-SPM-Ctag

Plasmid

#163613

Purpose

Expresses truncated human Ornithine Decarboxylase Antizyme fused to SPM in bacterial cells for calcium-induced covalent coupling to Ornithine Decarboxylase.

Depositor

Mark Howarth

Article

Scheu et al Nat Commun. 2021 Jan 29;12(1):717. doi:

Insert

OAZ-GSY-SPM

Tags

C-tag and His6

Expression

Bacterial

Promoter

T7

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pSG5-FLAG-CaMKKbeta rat K193A

Plasmid

#32450

Depositor

Anthony Means

Article

Anderson et al Cell Metab. 2008 May;7(5):377-88.

Insert

Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)

Tags

FLAG

Expression

Mammalian

Mutation

K193A (S3V, G96R, and P108L also present)

Promoter

SV40

Available Since

April 17, 2012

Availability

Academic Institutions and Nonprofits only
pMSP1D1T1

Plasmid

#97327

Purpose

Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional T residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.

Depositor

Michael Marty

Article

Reid et al Anal Chem. 2017 Nov 7;89(21):11189-11192

Insert

MSP1D1T1

Tags

6xHis - TEV

Expression

Bacterial

Mutation

Addition of T residue after T25

Promoter

T7

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 CAMK1

Plasmid

#67075

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

CAMK1 (CAMK1 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pSG5-FLAG-CaMKKbeta rat D311A

Plasmid

#32451

Depositor

Anthony Means

Article

Anderson et al Cell Metab. 2008 May;7(5):377-88.

Insert

Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)

Tags

FLAG

Expression

Mammalian

Mutation

D311A (S3V, G96R, and P108L also present)

Promoter

SV40

Available Since

April 17, 2012

Availability

Academic Institutions and Nonprofits only
SBC015898

Plasmid

#226285

Purpose

Expresses PhCFAT, ObEGS1, and ObEOMT1 from trc promoter. Biosynthesis of methylated phenylpropenes from monolignol precursors.

Depositor

Rainer Breitling

Article

Chua et al Metab Eng Commun. 2024 May 15;18:e00237.

Inserts

Coniferyl alcohol acyltransferase
Eugenol synthase 1
Eugenol O-methyltransferase

Expression

Bacterial

Available Since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-GCaMP6f-WPRE

Plasmid

#216275

Purpose

AAV vector with hSynapsin promoter and DIO Lox sites for Cre-On expression of green fluorescent calcium indicator GCaMP6f

Depositor

Guohong Cui

Article

Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do

Insert

GCaMP6f

Use

AAV and Cre/Lox

Tags

6XHIS and Xpress

Promoter

human Synapsin 1 (hSyn)

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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(I) pADH2 (J) P3 (SBE114)

Plasmid

#187629

Purpose

promoter of the gene alcohol dehydrogenase 2 from R. toruloides for the P3 position (I/J)

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(I) pADH2 (J)

Expression

Bacterial

Available Since

Dec. 7, 2023

Availability

Academic Institutions and Nonprofits only

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(F) pADH2 (G)_P2 (SBE113)

Plasmid

#187628

Purpose

promoter of the gene alcohol dehydrogenase 2 from R. toruloides fro the P2 position (F/G)

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(F) pADH2 (G)

Expression

Bacterial

Available Since

Dec. 7, 2023

Availability

Academic Institutions and Nonprofits only

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(R)pADH2(D)_P1(SBE214)

Plasmid

#195015

Purpose

promoter of the gene alcohol dehydrogenase 2 from R. toruloides for the position P1

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(B) pADH2 (R)

Expression

Bacterial

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pNMSB88

Plasmid

#199321

Purpose

Combine with Gal4 to direct TeNL expression

Depositor

Michael Krieg

Article

Porta-de-la-Riva et al Nat Methods. 2023 Apr 6. doi: 10.1038/s4

Insert

15xUAS::delta pes-10::::TeNL::let-858 3'UTR

Expression

Worm

Mutation

KD for calcium is 250 nM, 1 synthetic intron

Promoter

15xUAS::delta pes-10

Available Since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pNMSB109

Plasmid

#199322

Purpose

Combine with Gal4 to direct CaMBI 300 expression

Depositor

Michael Krieg

Article

Porta-de-la-Riva et al Nat Methods. 2023 Apr 6. doi: 10.1038/s4

Insert

15xUAS::delta pes-10::::CaMBI::let-858 3'UTR

Expression

Worm

Mutation

Orange CaMBI with 300 nM KD for calcium

Promoter

15xUAS:::delta pes-10

Available Since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

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