We narrowed to 10,458 results for: T7

1. pTXG

   Plasmid

   #52280

   Purpose

   Bacterial expression of TARGET proteins C-terminally fused to GyrA intein and GST

   Depositor

   Primo Schaer

   Article

   Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

   Type

   Empty backbone

   Use

   Tags

   GyrA-intein-GST

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. 6ASB (FBXL19)

   Plasmid

   #119954

   Purpose

   Bacterial expression for structure determination

   Depositor

   Cheryl Arrowsmith

   Insert

   FBXL19 (FBXL19 Human)
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   contains amino acids 31-153

   Promoter

   T7

   Available since

   Jan. 8, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pCARSF63

   Plasmid

   #64695

   Purpose

   H6-TRX-Smt3-GB1-NpuDnaEN102-CBD

   Depositor

   Hideo Iwai

   Article

   Guerrero et al Protein Expr Purif. 2015 Aug 20. pii: S1

   Type

   Empty backbone

   Use

   Tags

   CBD, HisTag, NpuDnaE-N102, Smt3, and TRX

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pACYCDUET-StrepSUMO-DaPFOR

   Plasmid

   #217819

   Purpose

   Expresses DaPFOR in E. coli

   Depositor

   Pamela Silver

   Article

   Ng et al ACS Chem Biol. 2024 Feb 16;19(2):380-391

   Insert

   pACYCDUET-StrepSUMO-DaPFOR
   

   Use

   Tags

   StrepSUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLTE EGFP

   Plasmid

   #67044

   Purpose

   Cell-free expression control

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   EGFP
   

   Use

   Cell-free expression

   Tags

   Myc

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pET29b-Ava3858-3856

   Plasmid

   #45745

   Depositor

   Christopher T Walsh, Emily Balskus

   Article

   Balskus et al Science. 2010 Sep 24;329(5999):1653-6. d

   Insert

   Ava3858-3856
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 8, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pET22b-EC12WTC9AAvi

   Plasmid

   #47925

   Purpose

   Expresses E-cadherin EC12 WT with C9A mutation and avi tag in E.Coli.

   Depositor

   Tanja Kortemme

   Article

   Ritterson et al J Am Chem Soc. 2013 Aug 7.

   Insert

   E-Cadherin EC12
   

   Use

   Tags

   6xHis, Avi Tag, and TEV Cleavage

   Expression

   Bacterial

   Mutation

   changed Cysteine 9 to Alanine

   Promoter

   T7

   Available since

   Sept. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28a-SUMO-SpyTag003

   Plasmid

   #216282

   Purpose

   Expresses SUMO with a C-terminal SpyTag003 in bacterial cells

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   SUMO-SpyTag003
   

   Use

   Tags

   His6 and SpyTag003

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. 6C1A (MBD2)

   Plasmid

   #119958

   Purpose

   Bacterial expression for structure determination

   Depositor

   Cheryl Arrowsmith

   Insert

   MBD2 (MBD2 Human)
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 8, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pET28-6His-SUMO-Nsp14

    Plasmid

    #154956

    Purpose

    Expresses Nsp14 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coli

    Depositor

    Justin Kollman

    Insert

    6His-SUMO-Nsp14 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    6His-Thrombin-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pET22b-EC12WTC9A

    Plasmid

    #47924

    Purpose

    Expresses E-cadherin EC12 WT with C9A mutation in E.Coli.

    Depositor

    Tanja Kortemme

    Article

    Ritterson et al J Am Chem Soc. 2013 Aug 7.

    Insert

    E-Cadherin EC12
    

    Use

    Tags

    6xHis and TEV Cleavage

    Expression

    Bacterial

    Mutation

    changed Cysteine 9 to Alanine

    Promoter

    T7

    Available since

    Sept. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCOM4

    Plasmid

    #49798

    Purpose

    ePathBrick vector for pathway engineering in E. coli.

    Depositor

    Mattheos Koffas

    Article

    Xu et al ACS Synth Biol. 2012 Jul 20;1(7):256-66.

    Type

    Empty backbone

    Use

    Tags

    S-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. CBX4

    Plasmid

    #25237

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    CBX4_02 (CBX4 Human)
    

    Use

    Tags

    6x His and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 13, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

14. 4LEC

    Plasmid

    #60091

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    METTL21A:JMC072-B07:C227766 (METTL21A Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    See comments

    Promoter

    T7

    Available since

    Oct. 22, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

15. SIRT5H158Y-pET15b

    Plasmid

    #81032

    Purpose

    Bacterial expression of Human catalytic mutant SIRT5 protein

    Depositor

    David Lombard

    Article

    Kumar et al Methods Mol Biol. 2016;1436:241-57. doi:

    Insert

    Sirt5 (SIRT5 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    H158Y catalytic mutant

    Promoter

    T7

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET29b-Ava3858-3855

    Plasmid

    #45746

    Depositor

    Christopher T Walsh, Emily Balskus

    Article

    Balskus et al Science. 2010 Sep 24;329(5999):1653-6. d

    Insert

    Ava3858-3855
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

17. HisMS2_VLP_FluA

    Plasmid

    #175955

    Purpose

    Generation of MS2 Virus-Like Particles packaged with the sequence for the Influenza A Matrix Protein [A/Illinois/20/2018(H1N1)]

    Depositor

    Paul Freemont

    Article

    Unruh et al medRxiv 2024.04.17.24305965

    Insert

    M gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. puC19 preACT1(WT)

    Plasmid

    #111323

    Purpose

    for T7 transcription of wt actin pre-mRNA

    Depositor

    Christine Guthrie

    Article

    Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

    Insert

    ACT1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28b-Ava3855

    Plasmid

    #45743

    Depositor

    Christopher T Walsh, Emily Balskus

    Article

    Balskus et al Science. 2010 Sep 24;329(5999):1653-6. d

    Insert

    Ava3855 (Ava_3855 Anabaena variabilis)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 3, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pET28b-PaODH

    Plasmid

    #110802

    Purpose

    Expresses PaODH and opine dehydrogenase from Pseudomonas aeruginosa

    Depositor

    Audrey Lamb

    Article

    McFarlane et al Biochemistry. 2017 Nov 14;56(45):5967-59

    Insert

    Pa4835
    

    Use

    Tags

    Hexahistidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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