We narrowed to 7,125 results for: trac

1. pMSCV mKeima-LC3

   Plasmid

   #189006

   Purpose

   Fluorescent reporter for autophagy

   Depositor

   Atsushi Hoshino

   Article

   Minami et al Nat Commun. 2023 Jul 13;14(1):4084. doi:

   Insert

   mKeima, LC3 (Map1lc3b Rat)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. FUW-C-prM-E-NS1

   Plasmid

   #175281

   Purpose

   lentiviral vector mediating bicistronic expression of the 4 genes of YFV-17D (C-prM-E-NS1) and EGFP.

   Depositor

   Wei Xu

   Article

   Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

   Insert

   C-prM-E-NS1 and EGFP (POLY Yellow fever virus strain 17D)
   

   Use

   Lentiviral

   Tags

   IRES EGFP

   Expression

   Mutation

   Promoter

   human ubiquitin C

   Available since

   Dec. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET30a-His6-LOXCATmut

   Plasmid

   #140085

   Purpose

   A control for His6-LOXCAT where LOX is catalytically dead (mutations H265A and R268A)

   Depositor

   Vamsi Mootha

   Article

   Patgiri et al Nat Biotechnol. 2020 Jan 13. pii: 10.103

   Insert

   A fusion of lactate oxidase from A. viridans and catalase from E. coli
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Mutation in H265A and R268A in LOX (used original…

   Promoter

   T7

   Available since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. SaCas9v2-Puro

   Plasmid

   #178802

   Purpose

   SaCas9 with 2A-Puro, and a cloning backbone for sgRNA.

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh (Cas9-2A Puro) and U6 (gRNA)

   Available since

   Aug. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-mPpp3cc-FLAG-6XHIS

   Plasmid

   #179160

   Purpose

   Expression vector of mouse protein phosphatase 3, catalytic subunit, gamma isoform (Ppp3cc) tagged with FLAG and 6X HIS at C-terminus, CAG promoter, rabbit globin poly(A) signal.

   Depositor

   Masahito Ikawa

   Article

   Miyata et al Science. 2015 Oct 23;350(6259):442-5. do

   Insert

   protein phosphatase 3, catalytic subunit, gamma isoform (Ppp3cc Mouse)
   

   Use

   Tags

   6XHIS and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFPC1-ORAI1-EC-HA

   Plasmid

   #174335

   Purpose

   encodes hORAI1 with HA-tag in its extracellular loop 2 and an N-terminal GFP fusion (GFP-ORAI1-EC-HA).

   Depositor

   Sonal Srikanth

   Article

   Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

   Insert

   human Orai1 (ORAI1 Human)
   

   Use

   Tags

   GFP in the N-terminus and HA tag in the extracell…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHBS1449 SED1-Lenti

   Plasmid

   #180824

   Purpose

   SED1 biosensor for stable integration and expression in mammalian cells

   Depositor

   Rajat Rohatgi

   Article

   Cuevas-Velazquez et al Nat Commun. 2021 Sep 14;12(1):5438. doi:

   Insert

   SED1 osmosensor
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   April 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV-hSyn-Axon-jGCaMP8m

   Plasmid

   #172719

   Purpose

   AAV transfer plasmid for neuronal expression of axon-targeted jGCaMP8m.

   Depositor

   Marianne Fyhn

   Article

   Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

   Insert

   Axon-jGCaMP8m
   

   Use

   AAV

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. aMHC-mCherry-Rex-Blasticidin

   Plasmid

   #21228

   Depositor

   Mark Mercola

   Article

   Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

   Insert

   alpha myosin heavy chain promoter
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   Available since

   June 30, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. SuExp His-Myc-mPLD3

    Plasmid

    #173853

    Purpose

    Protein expression plasmid for recombinant His-Myc tag mouse PLD3

    Depositor

    David Nemazee

    Article

    Gavin et al Nat Commun. 2021 Oct 7;12(1):5874. doi:

    Insert

    PLD3 (Pld3 Mouse)
    

    Use

    Tags

    6xHis, Myc, Factor X cleavable

    Expression

    Mammalian

    Mutation

    intracellular and transmembrane domain truncated,…

    Promoter

    CMV

    Available since

    Jan. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTT3 Unc5A-FLAG

    Plasmid

    #72194

    Purpose

    Expresses full-length Unc5a with a C-terminal FLAG tag

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Unc5a (Unc5a Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGPTVII-nlsSED1

    Plasmid

    #178190

    Purpose

    SED1 biosensor for expression in Arabidopsis thaliana

    Depositor

    Jose Dinneny, Cesar Cuevas-Velazquez

    Article

    Cuevas-Velazquez et al Nat Commun. 2021 Sep 14;12(1):5438. doi:

    Insert

    SED1 biosensor
    

    Use

    Tags

    SV40 NLS

    Expression

    Plant

    Mutation

    Promoter

    pUBQ10

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTT3 Unc5B-FLAG

    Plasmid

    #72195

    Purpose

    Expresses full-length Unc5b with a C-terminal FLAG tag

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Unc5b (Unc5b Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. cyt-GafD-mTurboID-V5

    Plasmid

    #184640

    Purpose

    Expresses V5-tagged GlycoID construct: GafD_short-miniTurboID in mammalian cytosol for O-GlcNAc interactome tagging

    Depositor

    Charlie Fehl

    Article

    Liu et al ACS Chem Biol. 2022 Jul 12. doi: 10.1021

    Insert

    cyt-GlycoID-V5
    

    Use

    Tags

    Nuclear export sequence and V5 tag

    Expression

    Mammalian

    Mutation

    GafD -> expressed aa22-178 ; BirA* -> expre…

    Promoter

    CMV

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEMS2155

    Plasmid

    #141065

    Purpose

    AAV plasmid with SYN1 promoter driving expression of EmGFP. Contains WPRE.

    Depositor

    Elizabeth Simpson

    Article

    Galvan et al Hum Gene Ther. 2021 Apr 16. doi: 10.1089

    Insert

    SYN1-EmGFP-WPRE
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    human Synapsin

    Available since

    April 28, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. eGFP-KIF1A-Rigor

    Plasmid

    #172207

    Purpose

    Expresses KIF1A G251A rigor mutant tagged with eGFP in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Guardia et al Cell Rep. 2016 Nov 15;17(8):1950-1961. d

    Insert

    KIF1A (Kif1a Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Changed Glycine 251 to Alanine

    Promoter

    CMV

    Available since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pB80-KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)_P2A_iLID-mCherry-RAB11

    Plasmid

    #174644

    Purpose

    Optogenetic coupling of RAB11 to opto-kinesin to induce anterograde transport of recycling endosomes

    Depositor

    Lukas Kapitein

    Article

    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)-P2A-iLID-mCherry-RAB11
    

    Use

    Tags

    Venus-SSPB and iLID-mCherry

    Expression

    Mammalian

    Mutation

    mmKIF1A(aa1-365): Pro202Ala; mVenus: Met1Del, Thr…

    Promoter

    Chicken beta-actin

    Available since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. nuc-GafD-mTurboID-HA

    Plasmid

    #184641

    Purpose

    Expresses 3xHA-tagged GlycoID construct: GafD_short-miniTurboID in mammalian nucleus for O-GlcNAc interactome tagging

    Depositor

    Charlie Fehl

    Article

    Liu et al ACS Chem Biol. 2022 Jul 12. doi: 10.1021

    Insert

    nuc-GlycoID-HA
    

    Use

    Tags

    3xHA tag and Nuclear localization sequence

    Expression

    Mammalian

    Mutation

    GafD -> expressed 22-178 ; BirA* -> express…

    Promoter

    CMV

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDEST-HisMBP-SED1

    Plasmid

    #178191

    Purpose

    SED1 biosensor for expression in Escherichia coli

    Depositor

    Jose Dinneny, Cesar Cuevas-Velazquez

    Article

    Cuevas-Velazquez et al Nat Commun. 2021 Sep 14;12(1):5438. doi:

    Insert

    SED1 biosensor
    

    Use

    Tags

    6xHis-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. p51.SRE.mCMV.CD63-GFP.wpre

    Plasmid

    #226773

    Purpose

    CD63-GFP in p52 (SRE backbone)

    Depositor

    Ferdinando Pucci

    Article

    Gonzalez-Callejo et al PLoS One. 2023 Feb 3;18(2):e0279400. doi

    Insert

    CD63 (CD63 )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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