We narrowed to 7,412 results for: CIT

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pHH0103_SKAP2-1/1

Plasmid

#91447

Purpose

Protein expression and purification of human SH3 domain construct SKAP2-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SKAP2-1/1 (SKAP2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pOPINK-OTUD3 (OTU+UBA, aa 52-275)

Plasmid

#61411

Purpose

Expresses human OTUD3 (OTU+UBA domains) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUD3 (OTUD3 Human)

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-51 and aa 276-398.

Promoter

T7

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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plenti hSyn AldoA

Plasmid

#220916

Purpose

Neuronal specific expression of AldoA

Depositor

Timothy Ryan

Article

Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

Insert

AldoA (ALDOA Human)

Use

Lentiviral

Expression

Mammalian

Promoter

hSyn

Available Since

June 28, 2024

Availability

Academic Institutions and Nonprofits only

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pBSFI-myr-akt-K179M

Plasmid

#49188

Purpose

cDNA of Akt1 with myristyolation signal and point mutation from K to M at position 179 in cloning vector

Depositor

Peter Vogt

Article

Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(

Insert

Akt1 (Akt1 Mouse)

Use

Adaptor plasmid

Tags

HA tag and myristolation signal

Mutation

K179M

Promoter

T7

Available Since

Dec. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pOPINK-OTUD5 (OTU, aa 171-358)

Plasmid

#61415

Purpose

Expresses human OTUD5 (OTU domain) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUD5 (OTUD5 Human)

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-170 and aa 359-571.

Promoter

T7

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pRS416 Gal M337V YFP

Plasmid

#27449

Depositor

Aaron Gitler

Article

Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

Insert

TDP-43 (TARDBP Human)

Tags

YFP

Expression

Yeast

Mutation

Methionine 337 mutated to Valine (M337V)

Available Since

Feb. 10, 2011

Availability

Academic Institutions and Nonprofits only
pAAV-cTNT-Cre-miR122 TS

Plasmid

#209784

Purpose

Expresses Cre by the specific cTNT promoter and incorporation of the miR122 target sequences (miR122TS) into the 3’ untranslated region (3’ UTR)

Depositor

Yuxuan Guo

Article

Yang et al Circulation. 2024 May 28;149(22):1778-17

Insert

miR122 ts

Use

AAV

Promoter

cTNT

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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MoFGFR2b-ires neo

Plasmid

#86491

Purpose

mamalian cell expression of FGFR2b

Depositor

David Ornitz

Article

Ornitz et al J Biol Chem. 1996 Jun 21;271(25):15292-7

Insert

FgfrR2 IIIb 13A4 (FGFR2 Human)

Expression

Mammalian

Promoter

Moloney murine leukemia virus LTR

Available Since

Feb. 2, 2017

Availability

Academic Institutions and Nonprofits only

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ELF4_pet28a

Plasmid

#131661

Purpose

Bacterial expression of ETS gene ELF4

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ELF4 (ELF4 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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PARD3_PDZ_3

Plasmid

#103959

Purpose

Protein expression and purification of PAR3 PDZ3 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

PARD3 (PARD3 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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U6-Slc1a3 sgRNA; EF1a-dCas9-KRAB-GFP

Plasmid

#194283

Purpose

EF1a-dCas9-KRAB-GFP with Slc1a3 sgRNA

Depositor

Elizabeth Kirby

Article

Rieskamp et al iScience. 2023 Jun 8;26(7):107068. doi:

Insert

dCas9-KRAB-GFP

Use

CRISPR and Lentiviral

Tags

GFP

Promoter

EF1a

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-DIO-CFL-SN-P2A-GCaMP6f

Plasmid

#181742

Purpose

AAV vector for expressing CFL-SN and GCaMP6f

Depositor

Yasunori Hayashi

Article

Goto et al Science. 2021 Nov 12;374(6569):857-863.

Insert

CFL-SN-P2A-GCaMP6f

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

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All-in-one split-ADAR2(E488Q, N496F)

Plasmid

#170148

Purpose

AAV vector carrying ADAR2-DDC(E488Q, N496F)-λN-P2A-MCP-ADAR2-DDN with a nuclear export signal

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

ADAR2-DDC(E488Q, N496F)-λN-P2A-MCP-ADAR2-DDN

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-dLbCpf1-BE-YE1

Plasmid

#154145

Purpose

Expresses dLbCpf1-BE-YE1(dLbCas12a-BE-YE1) in mammalian cells.

Depositor

Jin-Soo Kim

Article

Kim et al Nat Commun. 2020 Aug 13;11(1):4072. doi:

Insert

dLbCpf1-BE-YE1 (dCas12a-BE-YE1)

Tags

SV40 NLS

Expression

Mammalian

Mutation

APOBEC1(W90Y+R126E), dLbCpf1(D832A+E925A+D1148A)

Promoter

CMV

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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PTPN13_PDZ_4

Plasmid

#103922

Purpose

Protein expression and purification of PTN13 PDZ4 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

PTPN13 (PTPN13 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pLV-RhoB Sensor (PKN-L59Q)-IRES-Blast

Plasmid

#222634

Purpose

Rho reporter, sensor dead

Depositor

Arnold Hayer

Article

Marshall-Burghardt et al Sci Adv. 2024 Sep 6;10(36):eadn6858. doi

Insert

RhoB Sensor (PKN-L59Q)

Use

Lentiviral

Mutation

PKN L59Q

Promoter

EF1a

Available Since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pMx/ATP11C-HA

Plasmid

#209237

Purpose

Mammalian expression of ATP11C

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

Insert

ATP11C (ATP11C Human)

Use

Retroviral

Tags

HA

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pETDuet-1 TIM8,13

Plasmid

#170282

Purpose

Expresses both yeast Tim8 and yeast Tim13 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli production

Depositor

Paul Schanda

Article

Sucec et al Sci Adv. 2020 Dec 18;6(51). pii: 6/51/ea

Insert

yeast Tim8 and Tim13 (TIM8 Budding Yeast)

Tags

TEV-cleavable N-terminal His tag on Tim13 (on bac…

Expression

Bacterial

Available Since

June 15, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ266

Plasmid

#164713

Purpose

Gateway entry clone (attL1 & attR5) PmCDA1-CBE_V01 for C-T base editing

Depositor

Yiping Qi

Article

Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

Insert

zCas9(D10A)-PmCDA1-UGI

Use

CRISPR; Gateway compatible pmcda1-cbe_v01 entry c…

Expression

Plant

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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VVT1

Plasmid

#65779

Purpose

The Joung Lab recommends using BPK2660 (Addgene plasmid 70709) instead of VVT1 as guide RNAs cloned into BPK2660 are more effective than this plasmid. Human expression plasmid for S. aureus Cas9 sgRNA

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

SaCas9 gRNA backbone, without spacer sequence

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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