We narrowed to 8,589 results for: Set

1. pAcrVIB1-cloDF-amp

   Plasmid

   #184843

   Purpose

   Expression of anti-CRISPR protein AcrVIB1 in bacteria.

   Depositor

   Chase Beisel

   Article

   Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

   Insert

   anti-CRISPR protein inhibiting Cas13b nucleases
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   J23119

   Available Since

   Jan. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMSCV puro PRDM14

   Plasmid

   #193677

   Purpose

   Constitutive retroviral expression of PRDM14

   Depositor

   William Hahn

   Article

   Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

   Insert

   PRDM14 (PRDM14 Human)
   

   Use

   Retroviral

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLX304 PRDM14

   Plasmid

   #193688

   Purpose

   Constitutive lentiviral expression of PRDM14

   Depositor

   William Hahn

   Article

   Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

   Insert

   PRDM14 (PRDM14 Human)
   

   Use

   Lentiviral

   Available Since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC19-sg-1

   Plasmid

   #190607

   Purpose

   Expresses a gRNA for base editing the EGFP Kozak sequence of pWPT-/mEGFP-1T-IRES-mCherry

   Depositor

   Alessandro Quattrone

   Article

   Ambrosini et al Nucleic Acids Res. 2022 Oct 14;50(18):10

   Insert

   gRNA sequence
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC19-sgNCF1

   Plasmid

   #190193

   Purpose

   Expresses a gRNA for base editing the Kozak sequence of the human NCF1 gene

   Depositor

   Alessandro Quattrone

   Article

   Ambrosini et al Nucleic Acids Res. 2022 Oct 14;50(18):10

   Insert

   gRNA sequence
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDGO186N-KS3

   Plasmid

   #174303

   Purpose

   Replicating plasmid with sgRNA cassette containing a killing spacer #3 targeting the wild type polC gene.

   Depositor

   Lee Lynd

   Article

   Lanahan et al Appl Environ Microbiol. 2022 Jan 11;88(1

   Insert

   spacer expression cassette
   

   Expression

   Bacterial

   Available Since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAcrVIB1-cloDF-kan

   Plasmid

   #184836

   Purpose

   Expression of anti-CRISPR protein AcrVIB1 in bacteria.

   Depositor

   Chase Beisel

   Article

   Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

   Insert

   anti-CRISPR protein inhibiting Cas13b nucleases
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   J23119

   Available Since

   Oct. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pVH616

   Plasmid

   #169605

   Purpose

   Tier-2 vector encoding PmPGK1-driven Fluc and PCREm4-driven Nluc expression (PmPGK1-Fluc-pA::A2-pA::PCREm4-Nluc-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PPGK-driven FLuc Luciferase expression cassette and cAMP response element-controlled NanoLuc Luciferase expression cassette
   

   Expression

   Mammalian

   Promoter

   PCREm4 / PPGK

   Available Since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pVH614

   Plasmid

   #169603

   Purpose

   Tier-2 vector encoding PmPGK1-driven Fluc and PCREm4-driven Nluc expression (PmPGK1-Fluc-pA::PCREm4-Nluc-pA::A3-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PPGK-driven FLuc Luciferase expression cassette and cAMP response element-controlled NanoLuc Luciferase expression cassette
   

   Expression

   Mammalian

   Promoter

   PCREm4 / PPGK

   Available Since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. ATP1A1_G3_EBFP_Nick_Dual_sgRNA

    Plasmid

    #178094

    Purpose

    Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and EBFP Nick sgRNAs from two independent U6 promoters.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G3 + EBFP nick sgRNAs
    

    Use

    CRISPR; Prime editing

    Expression

    Mammalian

    Promoter

    Tandem U6 promoters

    Available Since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. ATP1A1_G3_EGFP_Nick_Dual_sgRNA

    Plasmid

    #178095

    Purpose

    Control vector for coselection for PE3b in human cells. Tandem expression of ATP1A1 G3 and EGFP Nick sgRNAs from two independent U6 promoters.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G3 + EGFP nick sgRNAs
    

    Use

    CRISPR; Prime editing

    Expression

    Mammalian

    Promoter

    Tandem U6 promoters

    Available Since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFB_ROSA26_SpCas9_gRNAs

    Plasmid

    #174703

    Purpose

    Plasmid containing separate SpCas9 gRNAs for targeted excision of inserted STOP Cassette upstream of reporter alleles found in Ai14 and Ai6 mice. ITRs flank the cassette for easy vector creation.

    Depositor

    Beverly L. Davidson

    Article

    Lang et al Nat Commun. 2019 Jul 30;10(1):3415. doi:

    Insert

    SpCas9 dual gRNAs
    

    Use

    AAV

    Available Since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRMCE{PuroR-5FCS-GMRWhite}-Vasa-FC31

    Plasmid

    #165888

    Purpose

    Puromycin resistant FC31 integrase mediated RMCE cassette for upgrading MiMic insertions

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    All-in-one FC31 RMCE Cassette
    

    Use

    Synthetic Biology

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRMCE{BlastR-5FCS-GMRWhite}-Vasa-FC31

    Plasmid

    #165889

    Purpose

    Blasticidin resistant FC31 integrase mediated RMCE cassette for upgrading MiMic insertions

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    All-in-one FC31 RMCE Cassette
    

    Use

    Synthetic Biology

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMRBad-Z-C-DiB-RM

    Plasmid

    #168477

    Purpose

    C-fragment of the DiB-RM‐split‐Zip protein. This plasmid needs to be co-transformed with Plasmid 168475: pET11a-Z-N-DiB-RM to obtain DiB-RM‐split-Zip protein

    Depositor

    Jens Meiler

    Article

    Bozhanova et al PLoS Comput Biol. 2021 Nov 8;17(11):e100

    Insert

    Leucine Zipper + C-fragment of DiB-RM
    

    Expression

    Bacterial

    Promoter

    araBAD

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET11a-Z-N-DiB-RM

    Plasmid

    #168475

    Purpose

    N-fragment of the DiB-RM‐split‐Zip protein. This plasmid needs to be co-transformed with Plasmid 168477: pMRBad-Z-C-DiB-RM to obtain DiB-RM‐split-Zip protein

    Depositor

    Jens Meiler

    Article

    Bozhanova et al PLoS Comput Biol. 2021 Nov 8;17(11):e100

    Insert

    N-fragment of DiB-RM + Leucine Zipper
    

    Tags

    His-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. KG#920

    Plasmid

    #110937

    Purpose

    Expresses INS-22-Emerald in the cholinergic motor neuron DA9 for imaging Dense Core Vesicles in a single neuron in a living animal

    Depositor

    Kenneth Miller

    Article

    Edwards et al Genetics. 2018 Nov;210(3):947-968. doi:

    Inserts

    mig-13 promoter
    INS-22-Emerald
    unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
    

    Expression

    Bacterial

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUASTLOTattB_vhhGFP4::Nrv1::tagBFP

    Plasmid

    #163925

    Purpose

    Expression of GrabFP-B-extracellular under control of UAS or LOP enhancer

    Depositor

    Markus Affolter

    Article

    Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife

    Insert

    Fusion of vhhGPF4 (extracellular) nanobody with Nrv1 protein and tagBFP fluorophor
    

    Use

    Cre/Lox

    Tags

    tagBFP

    Expression

    Insect

    Available Since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET_4E2H.210

    Plasmid

    #155212

    Purpose

    Expresses 4E2H.210 de novo protein in E.coli

    Depositor

    Bruno Correia

    Article

    Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

    Insert

    4E2H.210
    

    Tags

    6x His Tag

    Expression

    Bacterial

    Available Since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. Y365A or A365Y inducible rescue

    Plasmid

    #131323

    Purpose

    gRNA to generate i-WT-r or i-MT-r systems

    Depositor

    Danny Reinberg

    Article

    Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

    Insert

    Y365A or A365Y inducible rescue
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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