We narrowed to 14,031 results for: TIM

1. mCherry-C1-ORP5deltaTMD-FKBP

   Plasmid

   #220073

   Purpose

   expresses ORP5 with the transmembrane domain deleted. The mCherry fluorophore and FKBP are attached to ORP5.

   Depositor

   Gerry Hammond

   Article

   Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

   Insert

   OSBPL5deltaTMD (OSBPL5 Human)
   

   Expression

   Mammalian

   Mutation

   deltaTMD

   Available Since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. #1.4 pre-G-SynapShot-TagBFP

   Plasmid

   #214717

   Purpose

   Expresses green #1.4 pre-SynapShot and TagBFP

   Depositor

   Won Do Heo

   Article

   Son et al Nat Methods. 2024 Jan 8. doi: 10.1038/s4

   Insert

   #1.4 pre-G-SynapShot
   

   Use

   AAV

   Tags

   TagBFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   March 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pME-QFGal4-SV40pA

   Plasmid

   #155118

   Purpose

   Middle entry vector containing QFGal4

   Depositor

   Bret Pearson

   Article

   Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

   Insert

   QFGal4
   

   Use

   Gateway middle entry vector

   Available Since

   Sept. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. BII-CPspaH2B

   Plasmid

   #133385

   Purpose

   Piggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)

   Depositor

   Blair Madison

   Article

   Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. tetO-TBX5

   Plasmid

   #46032

   Depositor

   John Gearhart

   Article

   Addis et al J Mol Cell Cardiol. 2013 Apr 13;60C:97-1

   Insert

   TBX5 (TBX5 Human)
   

   Use

   Lentiviral

   Tags

   His and V5

   Expression

   Mammalian

   Promoter

   tetO

   Available Since

   July 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAJM.474

   Plasmid

   #108526

   Purpose

   LuxR + PLuxB-YFP Reporter. OC6 sensor for use in non-Marionette strain.

   Depositor

   Christopher Voigt

   Article

   Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

   Insert

   LuxR + PLuxB-YFP
   

   Expression

   Bacterial

   Mutation

   VanR: T49I A111V P179S

   Available Since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET14-GFP30_Encap

   Plasmid

   #86406

   Purpose

   Expresses sfGFP tagged with a 30 amino acid CLP and encapsulin as an operon

   Depositor

   David Savage

   Article

   Cassidy-Amstutz et al Biochemistry. 2016 Jun 21;55(24):3461-8.

   Inserts

   sfGFP with a 30aa CLP tag
   Encapsulin
   

   Tags

   6x Histidine

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYL-kasOp-FnCas12a2

   Plasmid

   #177874

   Purpose

   Expresses Streptomyces codon optimized FnCas12a. Used to modify genome of Streptomyces.

   Depositor

   Yunzi Luo

   Article

   Zhang et al Front Bioeng Biotechnol. 2020 Jun 30;8:7

   Insert

   Streptomyces codon optimized FnCas12a
   

   Use

   CRISPR

   Promoter

   kasOp*

   Available Since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. LT3GECIR

   Plasmid

   #111178

   Purpose

   Tet-ON miR-E (miR-30 variant)-based RNAi

   Depositor

   Johannes Zuber

   Article

   Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

   Insert

   miR-E (miR-30 variant)
   

   Use

   Lentiviral

   Mutation

   WT

   Promoter

   T3G

   Available Since

   May 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pZR060_CROPseq-sgCtrl-CRISPRi

    Plasmid

    #180269

    Purpose

    Lentiviral CROPseq vector expressing non-targeting control sgRNA for CRISPRi

    Depositor

    Alexander Marson

    Article

    Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

    Insert

    No-Target Control sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTol2-nbt-mTagBFP2-P2A-mScarlet

    Plasmid

    #196115

    Purpose

    zebrafish expression of nbt-mTagBFP2-P2A-mScarlet

    Depositor

    Reinhard Köster

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    nbt-mTagBFP2-P2A-mScarlet
    

    Use

    Zebrafish expression

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. LT3REVIR

    Plasmid

    #111176

    Purpose

    Tet-ON miR-E (miR-30 variant)-based RNAi

    Depositor

    Johannes Zuber

    Article

    Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

    Insert

    miR-E (miR-30 variant)
    

    Use

    Lentiviral

    Mutation

    WT

    Available Since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCA11-pMa-sgTS3-17xMS2V5-sgTS3

    Plasmid

    #199442

    Purpose

    CRISPR donor plasmid to insert 17XMS2V5 into the ribosomal DNA region in dependent of homology arms

    Depositor

    Baohui Chen

    Article

    Fu et al J Cell Biol. 2023 Jan 2;222(1):e20220211

    Insert

    HITI donor template
    

    Use

    CRISPR

    Available Since

    May 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. XE107 XPKC alpha-GFP-CS2+

    Plasmid

    #16743

    Depositor

    Randall Moon

    Article

    Sheldahl et al Curr Biol. 1999 Jul 1;9(13):695-8. doi:

    Insert

    PKC-alpha (prkca Frog)
    

    Use

    Xenopus expression

    Tags

    GFP

    Available Since

    March 14, 2008

    Availability

    Academic Institutions and Nonprofits only

15. pSR59.4

    Plasmid

    #63175

    Purpose

    Expresses OmpR constitutively from PompB97 and sfGFP under the PompF146 promoter

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al ACS Synth Biol. 2014 Nov 21;3(11):820-31

    Inserts

    OmpR (ompR )
    sfGFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. ER-EGFP-eMagA

    Plasmid

    #162245

    Purpose

    Blue-light dependent heterodimerization system

    Depositor

    Pietro De Camilli

    Article

    Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

    Insert

    CytP450 ER targeting sequencel-EGFP-eMagA
    

    Expression

    Mammalian

    Promoter

    beta-actin promoter

    Available Since

    Jan. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBBRBB-eGFP

    Plasmid

    #32549

    Depositor

    Claudia Schmidt-dannert

    Article

    Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

    Insert

    eGFP (eGFP )
    

    Use

    Synthetic Biology

    Tags

    His

    Expression

    Bacterial

    Promoter

    Consitutive lac

    Available Since

    Nov. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. SGEN

    Plasmid

    #111171

    Purpose

    miR-E (miR-30 variant)-based RNAi

    Depositor

    Johannes Zuber

    Article

    Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

    Insert

    miR-E (miR-30 variant)
    

    Use

    Lentiviral

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-Syn-ChR2(H134R)-GFP

    Plasmid

    #58880

    Purpose

    AAV expression of ChR2(H134R)-GFP under the Syn promoter

    Depositor

    Edward Boyden

    Article

    Boyden et al Nat Neurosci. 2005 Sep . 8(9):1263-8.

    Has Service

    AAV Retrograde and AAV8

    Insert

    ChR2(H134R)-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    H134R

    Promoter

    Syn

    Available Since

    Aug. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHsp70-Cas9

    Plasmid

    #45945

    Purpose

    The codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter used in Gratz, et al. (2013). Plasmid is low copy number.

    Depositor

    Melissa Harrison, Kate O'Connor-Giles, Jill Wildonger

    Article

    Gratz et al Genetics. 2013 May 24.

    Insert

    Codon optimized Cas9
    

    Use

    CRISPR

    Tags

    3x FLAG

    Mutation

    Silent change from C to A at bp 834 of insert

    Available Since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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