We narrowed to 6,591 results for: human c myc

1. pET29-hMIGA2(170-593)-6xhis

   Plasmid

   #192834

   Purpose

   Express codon-optimized human MIGA2 fragment 170-593 with a C-terminal 6xhis tag (lipid transfer construct) in bacteria

   Depositor

   Karin Reinisch

   Article

   Hong et al J Cell Biol. 2022 Dec 5;221(12):e2022070

   Insert

   Codon-optimized human Mitoguardin 2 (MIGA2 Human)
   

   Tags

   6xhis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKK-TEV-mTagBFP

   Plasmid

   #105789

   Purpose

   Expression of your protein of interest in fusion with blue fluorescent protein at the C-terminus (cleavable by TEV) (PMID: 18940671, 25378625). Useful for multi-channel live cell imaging.

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   TEV-mTagBFP

   Expression

   Mammalian

   Available Since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET29-hMIGA2(306-593)-6xhis

   Plasmid

   #192837

   Purpose

   Express codon-optimized human MIGA2 fragment 306-593 with a C-terminal 6xhis tag (lipid transfer construct) in bacteria

   Depositor

   Karin Reinisch

   Article

   Hong et al J Cell Biol. 2022 Dec 5;221(12):e2022070

   Insert

   Codon-optimized human Mitoguardin 2 (MIGA2 Human)
   

   Tags

   6xhis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCA528 CHMP4C 156-233

   Plasmid

   #108257

   Purpose

   Expresses C-terminal 156-233 residues of CHMP4C with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-9

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   CHMP4C residues 156-233 (CHMP4C Human)
   

   Tags

   His-SUMO

   Expression

   Bacterial

   Mutation

   Contains nonnative Gly-Cys for fluorescent labeli…

   Promoter

   T7

   Available Since

   Nov. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCA528 CHMP5 159-222

   Plasmid

   #108259

   Purpose

   Expresses C-terminal 159-222 residues of CHMP5 with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-10

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   CHMP5 residues 148-219 (CHMP5 Human)
   

   Tags

   His-SUMO

   Expression

   Bacterial

   Available Since

   Nov. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCA528 CHMP1A 140-196

   Plasmid

   #104593

   Purpose

   Expresses C-terminal 140-196 residues of CHMP1A with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-1

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   CHMP1A residues 140-196 (CHMP1A Human)
   

   Tags

   His-SUMO

   Expression

   Bacterial

   Mutation

   Contains nonnative Gly-Cys for fluorescent labeli…

   Promoter

   T7

   Available Since

   Nov. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCA528 CHMP2A 152-222

   Plasmid

   #104594

   Purpose

   Expresses C-terminal 152-222 residues of CHMP2A with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-3

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   CHMP2A residues 152-222 (CHMP2A Human)
   

   Tags

   His-SUMO

   Expression

   Bacterial

   Mutation

   Contains nonnative Gly-Cys for fluorescent labeli…

   Promoter

   T7

   Available Since

   Nov. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCA528 CHMP2B 141-213

   Plasmid

   #104595

   Purpose

   Expresses C-terminal 141-213 residues of CHMP2B with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-4

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   CHMP2B residues 141-213 (CHMP2B Human)
   

   Tags

   His-SUMO

   Expression

   Bacterial

   Mutation

   Contains nonnative Gly-Cys for fluorescent labeli…

   Promoter

   T7

   Available Since

   Nov. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCA528 CHMP3 159-222

   Plasmid

   #104599

   Purpose

   Expresses C-terminal 159-222 residues of CHMP3 with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-5

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   CHMP3 residues 159-222 (CHMP3 Human)
   

   Tags

   His-SUMO

   Expression

   Bacterial

   Mutation

   Contains nonnative Gly-Cys for fluorescent labeli…

   Promoter

   T7

   Available Since

   Nov. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCA528 CHMP4C, residues 156-233, no Cys

    Plasmid

    #180642

    Purpose

    Bacterial expression for CHMP4C C-terminal tail, residues 156-233. Used for FP competition experiments. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-14.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CHMP4C (CHMP4C Human)
    

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    residues 316-366

    Promoter

    T7

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCA528 IST1, residues 315-360, no Cys

    Plasmid

    #180643

    Purpose

    Bacterial expression of IST1 C-terminal tail, residues 315-360. Used for FP competition experiments. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-99.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    IST1 (IST1 Human)
    

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    residues 315-360

    Promoter

    T7

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCEP4-BG505.SOSIP-gp160-QES.i05.c06-754*

    Plasmid

    #123275

    Purpose

    Mammalian expression plasmid for Env from the BG505 HIV-1 isolate (containing SOSIP mutations); C-terminal truncation; QES mutant for enhanced presentation of quaternary epitopes

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (BG505) Env
    

    Tags

    CD5 leader peptide

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene; SOSIP mutations; …

    Promoter

    CMV

    Available Since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKK-TEV-mAmber

    Plasmid

    #105792

    Purpose

    Expression of your protein of interest in fusion with non-absorbing, non-emitting Venus mutant at the C-terminus (cleavable by TEV). Negative control for FRET measurements (PMID: 17040988, 22264545).

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    TEV-mAmber

    Expression

    Mammalian

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPW3761 : CMVd3-HsIRE1(WLLI-GSGS)[359-362]-HaloTag

    Plasmid

    #185683

    Purpose

    Low-level transient expression of IF2 mutant HsIRE1 with a C-terminal HaloTag.

    Depositor

    Peter Walter

    Article

    Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

    Insert

    ERN1 (ERN1 Synthetic, Human)
    

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    (WLLI-GSGS)[359-362]

    Available Since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. peGFP C1-SPG20 SC domain

    Plasmid

    #218580

    Purpose

    Expresses GFP-tagged SC domain of human SPG20 in mammalian cells

    Depositor

    Hemmo Meyer

    Article

    Gahlot et al Mol Cell. 2024 Mar 14:S1097-2765(24)0017

    Insert

    SPG20 (SPART Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-417

    Promoter

    CMV

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFETCh_CEBPB

    Plasmid

    #64055

    Purpose

    Homology Arms and 3X Flag with P2A Neo for 3' tagging of human CEBPB

    Depositor

    Richard M. Myers, Eric Mendenhall

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    CEBPB Homology Arms (CEBPB Human)
    

    Tags

    3X Flag P2A Neo

    Available Since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFP-p97-E578Q

    Plasmid

    #85672

    Purpose

    Expression of human p97 E578Q mutant with N-terminal GFP tag

    Depositor

    Hemmo Meyer

    Article

    Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

    Insert

    p97 (Vcp Rat)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    E578Q

    Promoter

    CMV

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. peGFP C3-SPG20-PAAA

    Plasmid

    #218581

    Purpose

    Expresses GFP-tagged PPAY motif mutant (PAAA) of human SPG20 in mammalian cells; this mutant does not interact with ITCH

    Depositor

    Hemmo Meyer

    Article

    Gahlot et al Mol Cell. 2024 Mar 14:S1097-2765(24)0017

    Insert

    SPG20 (SPART Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    changed Proline 172 to Alanine and Tyrosine 174 t…

    Promoter

    CMV

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. peGFP-HDAC1 (pc2447)

    Plasmid

    #240148

    Purpose

    Expression vector containing a fusion protein of eGFP and human HDAC1.

    Depositor

    Cristina Cardoso

    Article

    Rausch et al Nucleic Acids Res. 2020 Dec 16;48(22):12

    Insert

    HDAC1 (HDAC1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. Cx30-msfGFP

    Plasmid

    #69019

    Purpose

    Expresses human Cx30 (GJB6 CDS) with a 7 amino acid linker on the C-terminus of the Connexin linking to monomerized superfolder Green Fluorescent Protein. Expression driven by CMV promoter.

    Depositor

    David Spray

    Article

    Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.

    Insert

    Cx30 (GJB6 Human)
    

    Tags

    V206K monomerized super folder GFP

    Expression

    Mammalian

    Promoter

    c

    Available Since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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