We narrowed to 11,932 results for: pcDNA

1. pcDNA5-FRT-TO-Nsp5-HF

   Plasmid

   #157687

   Purpose

   mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp5 under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   Nsp5-HF (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   His8-Flag

   Expression

   Mammalian

   Mutation

   codon optimized

   Promoter

   Available since

   Aug. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pcDNA TfR1-miniE

   Plasmid

   #181987

   Purpose

   MiniE genetically encoded tag on Transferrin Receptor 1 (TfR1) to be used with MiniR labeled with a reporter

   Depositor

   Kimberly Beatty

   Article

   Doh et al Biochemistry. 2020 Aug 25;59(33):3051-30

   Insert

   TfR1-MiniE
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1(+) Drp1 V2 S579A

   Plasmid

   #46342

   Depositor

   David Chan

   Article

   Loson et al Mol Biol Cell. 2013 Jan 2.

   Insert

   Drp1 V2 (Dnm1l Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S579A

   Promoter

   CMV

   Available since

   July 23, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA3-myc3-14-3-3 Beta

   Plasmid

   #19957

   Depositor

   Yue Xiong

   Article

   Shumway et al J Biol Chem. 2003 Jan 24. 278(4):2089-92

   Insert

   14-3-3 Protein Beta (YWHAB Human)
   

   Use

   Tags

   Myc3

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 1, 2009

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA4/TO/Strep-HA-ZAKalpha_K45A

   Plasmid

   #141194

   Purpose

   Expresses kinase-dead Strep-HA-ZAK alpha in mammalian cells, can be used to make inducible cell line

   Depositor

   Simon Bekker-Jensen

   Article

   Vind et al Mol Cell. 2020 Apr 9. pii: S1097-2765(20

   Insert

   MAP3K20 (MAP3K20 Human)
   

   Use

   Tags

   Strep, HA

   Expression

   Mammalian

   Mutation

   Changed Lysine 45 to Alanine

   Promoter

   CMV

   Available since

   June 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcdna3 Flag Jip2

   Plasmid

   #59027

   Purpose

   act as molecular scaffolds that mediate the activation of the JNK signaling pathway in vivo.

   Depositor

   Roger Davis

   Article

   Yasuda et al Mol Cell Biol. 1999 Oct;19(10):7245-54.

   Insert

   mitogen-activated protein kinase 8 interacting protein 2 (MAPK8IP2 )
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA4-Myc-eIF3f

   Plasmid

   #35975

   Depositor

   Susana Valente

   Article

   Valente et al Proc Natl Acad Sci U S A. 2009 Mar 17;10

   Insert

   eukaryotic translation initiation factor 3, subunit F (EIF3F Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 9, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-myc-AP1S3

   Plasmid

   #58292

   Purpose

   mammalian expression of AP1S3

   Depositor

   Francesca Capon

   Article

   Setta-Kaffetzi et al Am J Hum Genet. 2014 May 1;94(5):790-7.

   Insert

   AP1S3 (AP1S3 Human)
   

   Use

   Tags

   6xHis and myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 1, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA3.1(+)-Mito-CaNARi

   Plasmid

   #64851

   Purpose

   Mitochondria-targeted FRET biosensor for monitoring calcineurin (CaN) activation; targeted to cytosolic face of mitochondrial outer membrane.

   Depositor

   Jin Zhang

   Article

   Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

   Insert

   CaNARi
   

   Use

   Tags

   Cerulean, N-terminal 30 amino acids from DAKAP1, …

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1_PA-Cre-Y324F

    Plasmid

    #122961

    Purpose

    Catalytically inactive mutant of PA-Cre

    Depositor

    Moritoshi Sato

    Article

    Kawano et al Nat Chem Biol. 2016 Dec;12(12):1059-1064

    Insert

    pcDNA3.1_PA-Cre-Y324F
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA5-LAP-MPS1-KD

    Plasmid

    #114045

    Purpose

    Expression of exogenous LAP tagged MPS1 Kinase-dead

    Depositor

    Geert Kops

    Article

    Nijenhuis et al J Cell Biol. 2013 Apr 15;201(2):217-31.

    Insert

    MPS1-KD (TTK Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D664A kinase dead

    Promoter

    Available since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA5-TO-PV-NLS-Dsred

    Plasmid

    #16341

    Depositor

    Anton Bennett

    Article

    Rodrigues et al J Biol Chem. 2007 Jun 8. 282(23):17061-8

    Insert

    PV-DsRed-NLS (Pvalb Rat)
    

    Use

    Tags

    Myc and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

13. pCDNA3 ATP5O(5’UTR)-FF

    Plasmid

    #85486

    Purpose

    Firefly luciferase under the control of ATP5O 5'UTR

    Depositor

    Ivan Topisirovic

    Article

    Gandin et al Genome Res. 2016 May;26(5):636-48. doi:

    Insert

    5'UTR of ATP5O (ATP5PO Human)
    

    Use

    Luciferase

    Tags

    Firefly luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. TLS 5:hTLS-CHOP.pCDNA1

    Plasmid

    #21831

    Depositor

    David Ron

    Article

    Crozat et al Nature. 1993 Jun 17;363(6430):640-4.

    Insert

    TLS-CHOP (FUS Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA5/FRT/TO_LAMP1_STf

    Plasmid

    #182006

    Purpose

    CMV overexpression of SNAP-tag fast in the lysosome of mammalian cell lines

    Depositor

    Kai Johnsson

    Article

    Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

    Insert

    LAMP1_STf
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Fast mutation on SNAP-tag E30R

    Promoter

    CMV

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3 c-SRC (91-536)

    Plasmid

    #42206

    Depositor

    Robert Lefkowitz

    Article

    Miller et al J Biol Chem. 2000 Apr 14;275(15):11312-9

    Insert

    c-SRC (91-536) (SRC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    contains amino acids 91-536

    Promoter

    CMV

    Available since

    Feb. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA3.1(+)Gαi2-91-NLuc

    Plasmid

    #207149

    Purpose

    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI2 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

    Depositor

    Dominique Schols, Tom Van Loy

    Article

    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

    Insert

    GNAI2 (GNAI2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NLuc inserted at position L(91)Q

    Promoter

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-MAVS(KaganF57)

    Plasmid

    #52000

    Purpose

    Expresses the human MAVS CDS containing "weak translational context"

    Depositor

    Jonathan Kagan

    Article

    Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

    Insert

    MAVS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA3.1-MS2-HA-HsMex3C_AD

    Plasmid

    #148481

    Purpose

    Mammalian Expression of HsMex3C

    Depositor

    Elisa Izaurralde

    Insert

    HsMex3C (MEX3C Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    L11P in Mex3C

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA-Pom121 mApple

    Plasmid

    #187674

    Purpose

    In vivo visualization of NPC protein Pom121

    Depositor

    Mary Beckerle

    Insert

    Pom121 (Pom121 Mouse)
    

    Use

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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