We narrowed to 10,632 results for: t7

1. pRSETA_ionRD

   Plasmid

   #172924

   Purpose

   Ionic strength FRET sensor with mCerulean3 and mCitrine for E. coli expression.

   Depositor

   Arnold Boersma

   Article

   Liu et al ACS Chem Biol. 2017 Oct 20;12(10):2510-2

   Insert

   IonicStrengthSensorRD
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-21a(+)-BMTU UDG-6xHis

   Plasmid

   #172197

   Purpose

   expresses His-tagged uracil-DNA-glycosylase enzyme from marine bacterium BMTU 3346 for the purpose of cross contamination prevention in nucleic acid amplification reactions

   Depositor

   Andrea Pauli

   Insert

   BMTU UDG
   

   Tags

   6xHis tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. bfloGFPa1

   Plasmid

   #60749

   Purpose

   Bacterial expression of Amphioxus GFPa1

   Depositor

   Dimitri Deheyn

   Article

   Bomati et al Sci Rep. 2014 Jun 27;4:5469. doi: 10.103

   Insert

   GFPa1
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pCoofy1-psHaloTag1a

   Plasmid

   #232940

   Purpose

   Photoswichable HaloTag: psHaloTag1a variant

   Depositor

   Claire Deo

   Article

   Walterspiel et al bioRxiv 2024.12.18.629107

   Insert

   photoswitchable HaloTag 1a variant
   

   Tags

   His6x-HRV3C

   Expression

   Bacterial

   Mutation

   E143W, A258W with linkers L1 (FAG) and L2 (P)

   Promoter

   T7

   Available Since

   March 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28-6His-SUMO-Nsp10

   Plasmid

   #154959

   Purpose

   Expresses Nsp10 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coli

   Depositor

   Justin Kollman

   Insert

   6His-SUMO-Nsp10 (ORF1ab SARS-CoV-2)
   

   Tags

   6His-Thrombin-SUMO

   Expression

   Bacterial

   Promoter

   T7 Promoter

   Available Since

   Aug. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pET-24a-ELP[V-150]

   Plasmid

   #67015

   Purpose

   Expresses ELP[V-150] in E. coli

   Depositor

   Ashutosh Chilkoti

   Article

   Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

   Insert

   ELP[V-150]
   

   Tags

   His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNGFP-BC

   Plasmid

   #161956

   Purpose

   Used for rapid and efficient membrane protein precrystallization screening strategy utilizing green fluorescent protein and fluorescence-detection size-exclusion chromatography (FSEC).

   Depositor

   Eric Gouaux

   Article

   Kawate et al Structure. 2006 Apr;14(4):673-81. doi: 1

   Type

   Empty backbone

   Tags

   GFPuv His8

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCGFP-BC

   Plasmid

   #161959

   Purpose

   Used for rapid and efficient membrane protein precrystallization screening strategy utilizing green fluorescent protein and fluorescence-detection size-exclusion chromatography (FSEC).

   Depositor

   Eric Gouaux

   Article

   Kawate et al Structure. 2006 Apr;14(4):673-81. doi: 1

   Type

   Empty backbone

   Tags

   GFPuv His8

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSANG14-3F

   Plasmid

   #39265

   Depositor

   John McCafferty

   Article

   Martin et al BMC Biotechnol. 2006 Dec 7;6:46.

   Type

   Empty backbone

   Tags

   6xHis, Alkaline phosphatase, pelB leader, and tri…

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. BRD7 (BRD7A-c005)

    Plasmid

    #98245

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    BRD7A (BRD7 Human)
    

    Tags

    His-6-TEV

    Expression

    Bacterial

    Mutation

    pfam00439:Bromodomain 136-223

    Promoter

    T7

    Available Since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTW051d2

    Plasmid

    #116893

    Purpose

    high stringency SE-PACE AP

    Depositor

    David Liu

    Article

    Wang et al Nat Chem Biol. 2018 Aug 20. pii: 10.1038

    Inserts

    g3c
    T7 RNAP C-term R632S/Q649S
    

    Available Since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTW051d

    Plasmid

    #116892

    Purpose

    low stringency SE-PACE AP

    Depositor

    David Liu

    Article

    Wang et al Nat Chem Biol. 2018 Aug 20. pii: 10.1038

    Inserts

    g3c
    T7 RNAP C-term R632S/Q649S
    

    Available Since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBB

    Plasmid

    #53210

    Purpose

    Contains a 103bp telomeric track

    Depositor

    Jack Griffith

    Article

    Bower et al Biochemistry. 2014 Sep 2;53(34):5485-95.

    Insert

    TTAGGG(n) Track
    

    Promoter

    T7

    Available Since

    June 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28-6His-TEV-Nsp14

    Plasmid

    #154954

    Purpose

    Expresses Nsp14 from SARS-CoV-2 with a N-terminal TEV-protease cleavable 6His tag in E. coli

    Depositor

    Justin Kollman

    Insert

    6His-TEV-Nsp14 (ORF1ab SARS-CoV-2)
    

    Tags

    6His-TEV

    Expression

    Bacterial

    Promoter

    T7 Promoter

    Available Since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pWUKI 1+2

    Plasmid

    #79879

    Purpose

    Encodes Cas1+2 and a CRISPR array

    Depositor

    George Church

    Article

    Shipman et al Science. 2016 Jun 9. pii: aaf1175.

    Inserts

    Cas1
    Cas2
    CRISPR Array
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. KLHL40 (KBTBD5A-c006)

    Plasmid

    #98233

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    KBTBD5A (KLHL40 Human)
    

    Tags

    TEV-His6-Flag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET15b/Shigella flexneri IpaA

    Plasmid

    #46178

    Depositor

    Susan Craig

    Article

    Chen et al J Cell Biol. 2005 May 9;169(3):459-70.

    Insert

    IpaA
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. MAPK3

    Plasmid

    #73235

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    MAPK3 (MAPK3 Human)
    

    Tags

    His-6-TEV

    Expression

    Bacterial

    Mutation

    pfam00069:Pkinase 42-330

    Promoter

    T7

    Available Since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLJSRSF7

    Plasmid

    #64693

    Purpose

    His-MBP-Smt3-NpuDnaEN102-CBD

    Depositor

    Hideo Iwai

    Article

    Guerrero et al Protein Expr Purif. 2015 Aug 20. pii: S1

    Type

    Empty backbone

    Tags

    CBD, HisTag, MBP, NpuDnaE-N102, and Smt3

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET22-Nsp10-TEV-6His

    Plasmid

    #154957

    Purpose

    Expresses Nsp10 from SARS-CoV-2 with a C-terminal TEV-protease cleavable 6His tag in E. coli

    Depositor

    Justin Kollman

    Insert

    Nsp10-TEV-6His (ORF1ab SARS-CoV-2)
    

    Tags

    TEV-6His

    Expression

    Bacterial

    Promoter

    T7 Promoter

    Available Since

    July 21, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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