We narrowed to 5,266 results for: 290

1. pHH0103_AHI1-1/1

   Plasmid

   #91269

   Purpose

   Protein expression and purification of human SH3 domain construct AHI1-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   AHI1-1/1 (AHI1 Human)
   

   Expression

   Bacterial

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pF708-pET28a-Hs-delta38-METTL20-NHis wt

   Plasmid

   #85109

   Purpose

   expression of recombinant human mature (delta38) METTL20 wt with N-terminal 6xHis-tag

   Depositor

   Pal Falnes

   Article

   Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

   Insert

   human mature (delta38) METTL20 (METTL20 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCWXPGR-pTF-DVL2deltaDIX

   Plasmid

   #114276

   Purpose

   TET-inducible expression of DVL2 protein with a deletion of its DIX-domain

   Depositor

   Patrick Salmon

   Article

   Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

   Insert

   DVL2 (Dvl2 Mouse)
   

   Use

   Lentiviral

   Tags

   FLAG

   Mutation

   deletion of DIX domain

   Available Since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV2_hSyn_Phobos_Citrine

   Plasmid

   #98216

   Purpose

   Blue-shifted artificial anion conducting channelrhodopsin (aACR). Activation max 466 nm; off-kinetics 10 ms. Codon optimized for mammalian expression.

   Depositor

   Simon Wiegert

   Article

   Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

   Insert

   Synthetic construct Phobos gene
   

   Use

   AAV

   Tags

   Citrine

   Expression

   Mammalian

   Mutation

   T59S, E83N, E90Q, E101S, V117R, E123S, T159G, G16…

   Promoter

   human synapsin

   Available Since

   Aug. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRB144

   Plasmid

   #180175

   Purpose

   pCDNA3.1 Construct GluN1 A714L

   Depositor

   Richard Bergeron

   Article

   Cappelli et al iScience. 2021 Dec 3;25(1):103539. doi:

   Insert

   GluN1 (Grin1 Rat)
   

   Tags

   Phluorin

   Expression

   Mammalian

   Mutation

   A714L

   Available Since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV V5-TbID-emerin

   Plasmid

   #175099

   Purpose

   Lentiviral expression of V5-TbID-tagged mouse Emd

   Depositor

   Larry Gerace

   Article

   Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

   Insert

   Emerin (Emd Mouse)
   

   Use

   Lentiviral

   Tags

   V5-TbID

   Expression

   Mammalian

   Available Since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLKO_NWS.Ollas.S_mCherry-NLS

   Plasmid

   #178279

   Purpose

   Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

   Depositor

   Brian Brown

   Article

   Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

   Insert

   nPC Tagged mCherry-NLS
   

   Use

   Lentiviral

   Tags

   NWS.Ollas.S and Nuclear Localization Signal

   Promoter

   eF1a

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLKO_HSV.S.V5_mCherry-NLS

   Plasmid

   #178278

   Purpose

   Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

   Depositor

   Brian Brown

   Article

   Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

   Insert

   nPC Tagged mCherry-NLS
   

   Use

   Lentiviral

   Tags

   HSV.S.V5 and Nuclear Localization Signal

   Promoter

   eF1a

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLKO_HSV.Ollas.V5_mCherry-NLS

   Plasmid

   #178277

   Purpose

   Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

   Depositor

   Brian Brown

   Article

   Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

   Insert

   nPC Tagged mCherry-NLS
   

   Use

   Lentiviral

   Tags

   HSV.Ollas.V5 and Nuclear Localization Signal

   Promoter

   eF1a

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLKO_HSV.NWS.S_mCherry-NLS

    Plasmid

    #178273

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    HSV.NWS.S and Nuclear Localization Signal

    Promoter

    eF1a

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lenti-sgTsc1#1/Cre

    Plasmid

    #173657

    Purpose

    Expresses a Tsc1-targeting gRNA and Cre-recombinase

    Depositor

    Charles Swanton

    Article

    Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

    Insert

    gRNA targeting Tsc1 (Tsc1 Mouse)
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Available Since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. Lenti-sgTsc1#2/Cre

    Plasmid

    #173658

    Purpose

    Expresses a Tsc1-targeting gRNA and Cre-recombinase

    Depositor

    Charles Swanton

    Article

    Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

    Insert

    gRNA targeting Tsc1 (Tsc1 Mouse)
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Available Since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. Lenti-sgPtprd#2/Cre

    Plasmid

    #173648

    Purpose

    Expresses a Ptprd-targeting gRNA and Cre-recombinase

    Depositor

    Charles Swanton

    Article

    Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

    Insert

    gRNA targeting Ptprd (Ptprd Mouse)
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Available Since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. Lenti-sgPtprd#1/Cre

    Plasmid

    #173647

    Purpose

    Expresses a Ptprd-targeting gRNA and Cre-recombinase

    Depositor

    Charles Swanton

    Article

    Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

    Insert

    gRNA targeting Ptprd (Ptprd Mouse)
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Available Since

    May 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEGFP-N1_MeCP2(R111G)

    Plasmid

    #110187

    Purpose

    Expression of mouse MeCP2 (R111G) in mammalian cells

    Depositor

    Adrian Bird

    Article

    Tillotson et al Nature. 2017 Oct 19;550(7676):398-401. d

    Insert

    MeCP2_e2 FL [R111G] (mouse cDNA) (Mecp2 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Arg 111 Gly

    Promoter

    CMV

    Available Since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPN007

    Plasmid

    #91575

    Purpose

    Express sgRNA targeting human BCL11A

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    BCL11A (BCL11A Human)
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. 1068 pGL3 FasL promoter (no SV40 prom)

    Plasmid

    #9029

    Depositor

    William Sellers

    Article

    Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

    Insert

    Fas ligand promoter (FASLG Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Removed the SV40 promoter from pGL3

    Available Since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

18. JG674: CAG-human dLbCpf1(D832A)-NLS-3xHA-3xFLAG-DmrA(X1)

    Plasmid

    #104568

    Purpose

    Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to one DmrA domain

    Depositor

    Keith Joung

    Article

    Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

    Insert

    dLbCpf1(D832A)-DmrA(x1)
    

    Tags

    3x FLAG, 3x HA, and NLS

    Expression

    Mammalian

    Mutation

    D832A

    Promoter

    CAG

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEBTet-SNAP-Cep70

    Plasmid

    #136821

    Purpose

    Mammalian expression of the centrosomal protein Cep70 N-terminally fused to SNAP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    SNAP-Cep70 (CEP70 Synthetic, Human)
    

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Promoter

    CMV-TetO2

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGFP-N1_MeCP2(deltaN)

    Plasmid

    #110189

    Purpose

    Expression of mouse MeCP2 (deltaN) in mammalian cells

    Depositor

    Adrian Bird

    Article

    Tillotson et al Nature. 2017 Oct 19;550(7676):398-401. d

    Insert

    MeCP2_e2 delta N (mouse cDNA) _EGFP (Mecp2 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    delta N terminus

    Promoter

    CMV

    Available Since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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