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We narrowed to 14,223 results for: Cas9

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Showing: 2761 - 2780 of 14223 results

  1. pSH-Csy4-T2A-SpRFN-P2A-GFP-multi-gRNA

    Plasmid
    #85756
    Purpose
    All in one plasmid that expresses Csy4, S.pyogenes FokI-dCas9-NLS, and GFP. Also encodes for multiplexed gRNAs. Backbone derived from pSQT1601 (Addgene #53369).
    Depositor
    Lawrence Stanton
    Publication
    Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Csy4-T2A-SpRFN-P2A-GFP
    gRNA
    Use
    CRISPR
    Tags
    Csy4, Csy4 recognition sequence, FokI fusion via …
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    CMV and U6
    Available since
    Jan. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    85756-plasmid-map-sequence-id-170275

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  4. pRGEB32-BAR

    Plasmid
    #126072
    Purpose
    For CRISPR/Cas9 delivery in maize. Expresses Cas9 with rice ubiquitin promoter, BAR with 35S promoter, and sgRNA/PTG with rice snoRNA U3 promoter (pol III). Can produce 1 or more gRNAs.
    Depositor
    Charles Hunter
    Publication
    Hunter et al Plants (Basel). 2021 Apr 8;10(4). pii: p
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Plant
    Promoter
    35S for BAR; Rice UBI for Cas9; Rice snoRNA U3 fo…
    Available since
    June 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    126072-plasmid-map-sequence-id-247528

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  5. pCSD_2XNLS-SpCas9WT-NLS-3xHA-2XNLS-NmCas9WT-2XNLS

    Plasmid
    #107324
    Purpose
    Expresses SpCas9 fused to NmCas9 in mammalian cells
    Depositor
    Scot Wolfe
    Publication
    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9-NmCas9 fusion
    Use
    CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    SpCas9 is fused to NmCas9
    Promoter
    CMV IE94
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107324-plasmid-map-sequence-id-204720

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  6. gTBEv5

    Plasmid
    #220621
    Purpose
    vector for encoding an engineered glycosylase-based thymine (T) base editor (gTBEv5) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter
    Depositor
    Huawei Tong
    Publication
    Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nSpCas9(Nterm)-XTEN-hUNG2-XTEN-Cas9(Cterm)
    Expression
    Mammalian
    Mutation
    hUNG2(del1-88;Y156A,A214T,Q259A,Y284D), SpCas9(D1…
    Promoter
    hU6, EF1a
    Available since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    220621-plasmid-map-sequence-id-436953

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  7. gCBEv3

    Plasmid
    #220618
    Purpose
    vector for encoding an engineered glycosylase-based cytosine (C) base editor (gCBEv3) driven by EF1a promoter, guide RNAs compatible with SpG driven by hU6, mCherry driven by CBh promoter
    Depositor
    Huawei Tong
    Publication
    Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nSpCas9(Nterm)-XTEN-hUNG2-XTEN-Cas9(Cterm)
    Expression
    Mammalian
    Mutation
    hUNG2(del1-88;N213D,K184A,A214V), SpCas9(D10A)
    Promoter
    hU6, EF1a
    Available since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    220618-plasmid-map-sequence-id-436973

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  8. pU6-Universal.Sth.dCas9

    Plasmid
    #171088
    Purpose
    CRISPR interference. Recipient construct for cloning 20-nt spacers into the sgRNA scaffold compatible with Streptococcus thermophilus dCas9 (CRISPR1 system) via BsaI restriction sites.
    Depositor
    Sebastian Lourido
    Publication
    Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Toxoplasma U6 upstream region – spacer cloning site - sgRNA scaffold (compatible with S. thermophilus Cas9 CRISPR1)
    DHFR-TSc3
    Use
    Toxoplasma gondii expression
    Mutation
    Note: A S245F mutation is present but did not app…
    Promoter
    DHFR-TS (Toxoplasma gondii) and U6 (Toxoplasma go…
    Available since
    Oct. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    171088-plasmid-map-sequence-id-335785

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  11. SaCas9_sgRNA_expression_in_pBluescript

    Plasmid
    #122090
    Purpose
    U6 driven SaCas9 sgRNA expression vector for cloning own guides
    Depositor
    Scot Wolfe
    Publication
    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SaCas9 tracr (NEWENTRY Synthetic, S. pyogenes)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    April 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    122090-plasmid-map-sequence-id-242296

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  12. pLenti_EF1α-NES-Kinprola_PKA-mEGFP_bGH-PA term_EF1α_FLAG-SV40NLS-Cas9-NLS-T2A-BSD-WPRE-SV40

    Plasmid
    #233371
    Purpose
    EF1α driven co-expression of the PKA activity recorder Kinprola_PKA fused to mEGFP and Cas9 expression through lentivirus transduction
    Depositor
    Kai Johnsson
    Publication
    Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038
    Inserts
    NES-Kinprola_PKA-mEGFP
    FLAG-SV40NLS-Cas9-NLS-T2A-BSD
    Use
    Lentiviral
    Tags
    FLAG, NES, and mEGFP
    Promoter
    EF1α
    Available since
    March 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    233371-plasmid-map-sequence-id-466776

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  19. pCSD_2XNLS-SpCas9MT3-NLS-3XHA-2XNLS-NmdCas9-2XNLS

    Plasmid
    #107323
    Purpose
    Expresses SpCas9 (R1335K) fused to dNmCas9 in mammalian cells
    Depositor
    Scot Wolfe
    Publication
    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9-NmCas9 fusion
    Use
    CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    SpCas9 (R1335K) is fused to nuclease dead NmCas9 …
    Promoter
    CMV IE94
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107323-plasmid-map-sequence-id-204717

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  20. iSpyMac

    Plasmid
    #155014
    Purpose
    Expresses the codon-optimized, engineered iSpyMac Cas9 nuclease in mammalian cells
    Depositor
    Joseph Jacobson
    Publication
    Chatterjee et al Nat Commun. 2020 May 18;11(1):2474. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    iSpyMac
    Expression
    Mammalian
    Mutation
    PID grafted onto SpyCas9 N-terminal domain, R221K…
    Promoter
    EF1ɑ
    Available since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    155014-plasmid-map-sequence-id-308820

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Showing: 2761 - 2780 of 14223 results