We narrowed to 26,603 results for: STI

1. MSP2279

   Plasmid

   #70706

   Purpose

   Bacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 2: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

   Inserts

   mammalian codon-optimized Staphylococcus aureus dCas9
   SaCas9 sgRNA (+84)
   

   Use

   CRISPR

   Tags

   NLS-3xFLAG

   Expression

   Bacterial

   Mutation

   D10A and H557A in SaCas9

   Promoter

   T7

   Available Since

   Dec. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. MTK0_047

   Plasmid

   #123977

   Purpose

   Encodes the Cas9 hCLYBL homology destination with Hygromycin resistance cassette GFP dropout destination vector as a type 0 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   hCLYBL CAS9 Destination - HygroR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX2T merlin 1-332

   Plasmid

   #11631

   Depositor

   Vijaya Ramesh

   Article

   Gonzalez-Agosti et al J Biol Chem. 1999 Nov 26. 274(48):34438-

   Insert

   merlin (NF2 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Amino-terminal half of merlin.

   Promoter

   Available Since

   May 3, 2006

   Availability

   Academic Institutions and Nonprofits only

4. pCMV-BD-cop1at(335-675)

   Plasmid

   #44973

   Depositor

   Thanos Halazonetis

   Article

   Crefcoeur et al Nat Commun. 2013;4:1779. doi: 10.1038/nc

   Insert

   BD-COP1 (COP1 Mustard Weed)
   

   Use

   Tags

   GAL4 DNA-binding domain

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-334

   Promoter

   pCMV

   Available Since

   June 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. Tol2pA2-drl:APEX2-mCherry

   Plasmid

   #188945

   Purpose

   Plasmid for Tol2 transgenesis expressing APEX2 tagged to mitochondria and nuclei, and cytoplasmic mCherry, driven by the draculin promoter

   Depositor

   Owen Tamplin

   Article

   Agarwala et al Elife. 2022 Aug 9;11. pii: 64835. doi: 1

   Insert

   drl:mito-APEX2_p2A_APEX2-H2B_p2A_mCherry_polyA
   

   Use

   Tol2 destination vector (attr4-r3)

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. MTK0_017

   Plasmid

   #123932

   Purpose

   Encodes the BxBI attB GFP dropout destination vector constitutive NLS::tagBFP as a type 0 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   BxBI attB tagBFP destination vector
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. MTK0_015

   Plasmid

   #123930

   Purpose

   Encodes the piggyBac GFP dropout destination vector with the 2xHS4 insulator and constitutive NLS::tagBFP as a type 0 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   PB Destination - Insulator + tagBFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. UAS::PTPRFb-DN-GFP

   Plasmid

   #37111

   Depositor

   Alvaro Sagasti

   Article

   Wang et al Curr Biol. 2012 Mar 6;22(5):373-82. Epub

   Insert

   UAS::PTPRFb-DN-GFP (ptprfb Zebrafish)
   

   Use

   Tol2 gateway expression vector

   Tags

   EGFP

   Expression

   Mutation

   Deleted aa1386-1909 corresponding to phosphatase …

   Promoter

   10xUAS

   Available Since

   July 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMC_mNG2(11)_BSDminus_F0

   Plasmid

   #184162

   Purpose

   Donor cassette plasmid for high-throughput tagging, encoding an mNG2(11) synthetic exon in frame 0, as well as a blasticidin resistance gene for selection of genomic integrants.

   Depositor

   Ophir Shalem

   Article

   Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

   Inserts

   mNG2(11)
   bsd
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. p6903 PHAGE-B CMV-N-EGFP Myc

    Plasmid

    #37608

    Depositor

    Peter Howley

    Article

    Martinez-Noel et al Mol Cell Biol. 2012 May 29.

    Insert

    Myc (MYC Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

11. lentiCRISPR-v2-Blast-Puro

    Plasmid

    #167186

    Purpose

    Lentiviral expression of sgRNA with blasticidin and puromycin resistance genes

    Depositor

    Yin Shen

    Article

    Ren et al Sci Adv. 2021 Sep 17;7(38):eabi4360. doi

    Insert

    Blasticidin S deaminase
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. PXPR007 sgGFP-2

    Plasmid

    #74961

    Purpose

    Cas9 + sgGFP-2 with blasticidin selection

    Depositor

    William Hahn

    Article

    Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

    Insert

    GFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-Tag 3B/WT myc-M87

    Plasmid

    #87722

    Purpose

    Expresses WT myc-M87 spastin isoform in mammalian cells

    Depositor

    Peter Baas

    Article

    Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

    Insert

    SPAST (SPAST Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    M87 isoform (deletion of nt 1-490 - 5'UTR an…

    Promoter

    CMV

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. FFiBlast MSCV Puro

    Plasmid

    #68464

    Purpose

    Retroviral plasmid for establishment of blasticidin resistance Cre-reporter cell line

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    Blasticidin Resistance
    

    Use

    Cre/Lox and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Inverted

    Promoter

    LTR

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. delta-EGFP

    Plasmid

    #119223

    Purpose

    GABAA receptor expression (delta subunit with EGFP tag at the C-terminus)

    Depositor

    Angel de Blas

    Article

    Christie et al Mol Cell Neurosci. 2006 Jan;31(1):1-14.

    Insert

    GABRD (Gabrd Rat)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX2T merlin 340-595

    Plasmid

    #11634

    Depositor

    Vijaya Ramesh

    Article

    Gonzalez-Agosti et al J Biol Chem. 1999 Nov 26. 274(48):34438-

    Insert

    merlin (NF2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Carboxy-terminal half of merlin isoform 1.

    Promoter

    Available Since

    May 3, 2006

    Availability

    Academic Institutions and Nonprofits only

17. pT7-SpCas9_sgRNA-site2 (RTW448)

    Plasmid

    #160137

    Purpose

    T7 promoter expression plasmid for in vitro transcription of SpCas9 sgRNA with spacer #2

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

    Insert

    SpCas9 sgRNA with spacer #2 (spacer=GTCGCCCTCGAACTTCACCT)
    

    Use

    In vitro transcription of sgrna from t7 promoter

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Feb. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. L2-AA012

    Plasmid

    #185751

    Purpose

    Vector to introduce eGFP under control of pAaEF1a into the genome of the hornwort Anthoceros agrestis via Agrobacterium mediated transformation. eGFP contains a cell membrane localization tag.

    Depositor

    Peter Szoevenyi

    Article

    Frangedakis et al New Phytol. 2021 Jun 2. doi: 10.1111/nph

    Inserts

    HygR2
    eGFP
    

    Use

    Tags

    membrane localization tag Lti6b

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMC_mNG2(11)_BSDminus_F1

    Plasmid

    #184163

    Purpose

    Donor cassette plasmid for high-throughput tagging, encoding an mNG2(11) synthetic exon in frame 1, as well as a blasticidin resistance gene for selection of genomic integrants.

    Depositor

    Ophir Shalem

    Article

    Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

    Inserts

    mNG2(11)
    bsd
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pME-hygro-hPGAP3-3HA

    Plasmid

    #50373

    Purpose

    Express C-terminally triple HA tagged human PGAP3 in mammmalian cells

    Depositor

    Reika Watanabe

    Article

    Castillon et al Mol Biol Cell. 2013 Jun;24(12):2021-33.

    Insert

    PGAP3 post-GPI attachment to proteins 3 (PGAP3 Human)
    

    Use

    Tags

    3x HA

    Expression

    Mammalian

    Mutation

    Promoter

    SR alpha

    Available Since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only