We narrowed to 229 results for: Ypet
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Plasmid#107081PurposeM. sedula PCNA3 fused at the C-terminus of YPetDepositorInsertM. sedula PCNA3 fused to YPet
TagsHis6 tagExpressionBacterialPromoterT7 promoterAvailable SinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET15b-YM2
Plasmid#107080PurposeM. sedula PCNA2 fused at the C-terminus of YPetDepositorInsertM. sedula PCNA2 fused to YPet
TagsHis6 tagExpressionBacterialPromoterT7 promoterAvailable SinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET15b-YM1
Plasmid#107079PurposeM. sedula PCNA1 fused at the C-terminus of YPetDepositorInsertM. sedula PCNA1 fused to YPet
TagsHis6 tagExpressionBacterialPromoterT7 promoterAvailable SinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDD283
Plasmid#66824PurposeYPET^SEC^3xFlag vector with ccdB markers for cloning homology armsDepositorInsertYPET-C1^SEC^3xFlag
UseCRISPR and Cre/LoxTags3xFlag and C. elegans codon-optimized YPETExpressionWormAvailable SinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
Desmoplakin II acceptor only control (F40-based tension sensor)
Plasmid#118719PurposeThe acceptor (mCherry) only control for the F40-based human desmoplakin II tension sensor can be co-expressed with the donor (YPet(short)) only control to determine intermolecular FRET.DepositorInserthuman Desmoplakin II-[YPet(short)(Y67G)-F40-mCherry] (internal-1353) (DSP Human)
UseRetroviralTagsYPet(short)(Y67G)-F40-mCherryExpressionMammalianMutationinserted F40-based tension sensor module after aa…PromoterCMVAvailable SinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVH664
Plasmid#169608PurposeTier-2 vector encoding PTetO7-driven SEAP-p2A-iRFP670, PmPGK1-driven rtTA and PmPGK1-driven YPet expression (PTetO7-CMVmin-2-SEAP-p2A-iRFP670-pA::PmPGK1-rtTA-pA::PmPGK1-YPet-pA).DepositorInserttetracycline-controlled SEAP and iRFP expression, PPGK-driven rtTA expression cassette and PPGK-driven YPet expression cassette
ExpressionMammalianPromotertetO7-PCMVmin / PPGK / PPGKAvailable SinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVH663
Plasmid#169607PurposeTier-2 vector encoding PTetO7-driven SEAP-p2A-iRFP670, PmPGK1-driven tTA and PmPGK1-driven YPet expression (PTetO7-CMVmin-2-SEAP-p2A-iRFP670-pA::PmPGK1-tTA-pA::PmPGK1-YPet-pA).DepositorInserttetracycline-controlled SEAP and iRFP expression, PPGK-driven tTA expression cassette and PPGK-driven YPet expression cassette
ExpressionMammalianPromotertetO7-PCMVmin / PPGK / PPGKAvailable SinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEM8734
Plasmid#213993PurposePrpsM driven expression of YpetDepositorInsertYpet
ExpressionBacterialPromoterPrpsMAvailable SinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJW1584
Plasmid#121055PurposeYPET^SEC^AID*::3xFLAG vector with ccdB sites for cloning homology arms.DepositorInsertYPET^SEC^AID*::3xFlag
UseCRISPR and Cre/LoxTags3xFLAG, C. elegans codon optimized YPET, and auxi…ExpressionWormAvailable SinceJune 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
Desmoplakin II tension sensor (F40-based)
Plasmid#118715PurposeThe F40-based human desmoplakin II tension sensor detects forces in the range of 1-6 pN by changes in FRET between YPet(short) and mCherry.DepositorInserthuman Desmoplakin II-[YPet(short)-F40-mCherry] (internal-1353) (DSP Human)
UseRetroviralTagsYPet(short)-F40-mCherryExpressionMammalianMutationinserted F40-based tension sensor module after aa…PromoterCMVAvailable SinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only