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Kif1bβ-pcDNA3

Plasmid

#22534

Depositor

William Kaelin

Article

Schlisio et al Genes Dev. 2008 Apr 1. 22(7):884-93.

Insert

Kif1bβ (KIF1B Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

wt cDNA

Promoter

Available since

Dec. 3, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA-14-3-3 ϵ

Plasmid

#48797

Purpose

expresses human myc-14-3-3ϵ in mammalian cells

Depositor

Huda Zoghbi

Article

Chen et al Cell. 2003 May 16;113(4):457-68.

Insert

YWHAE 14-3-3 ϵ (YWHAE Human)

Use

Tags

haemagglutinin

Expression

Mammalian

Mutation

Promoter

cmv

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only
pCDNA3-GFP

Plasmid

#74165

Purpose

Mammalian expression of EGFP

Depositor

Martine Roussel

Article

Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

Insert

GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-StagRFP

Plasmid

#173014

Purpose

Rationally enhanced red fluorescent protein derived from TagRFP-T. For mammalian expression.

Depositor

Jin Zhang

Article

Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

Insert

StagRFP

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-Nck1

Plasmid

#45892

Depositor

Louise Larose

Article

Kebache et al Proc Natl Acad Sci U S A. 2002 Apr 16;99

Insert

Nck-1 (NCK1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 16, 2014

Availability

Academic Institutions and Nonprofits only
FLAG-HA-pcDNA3.1-

Plasmid

#52535

Purpose

enables eukaryotic expression of N-terminally FLAG-HA-tagged fusion proteins

Depositor

Adam Antebi

Article

Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Tags

FLAG-HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 31, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNA3/GFP-PR

Plasmid

#20253

Depositor

Nico Dantuma

Article

Lindsten et al Antimicrob Agents Chemother. 2001 Sep .

Insert

HIV-1 protease

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

Available since

April 14, 2009

Availability

Academic Institutions and Nonprofits only

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pCDNA3 T7 Jip1

Plasmid

#51699

Purpose

JIP-1 may suppress signal transduction by the JNK pathway"

Depositor

Roger Davis

Article

Dickens et al Science. 1997 Aug 1. 277(5326):693-6.

Insert

Jnk interacting protein 1 (Mapk8ip1 Mouse)

Use

Tags

T7

Expression

Mammalian

Mutation

Promoter

Available since

March 14, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-Beclin1

Plasmid

#21150

Depositor

Junying Yuan

Article

Shibata et al J Biol Chem. 2006 May 19. 281(20):14474-

Insert

PcDNA3-Beclin1 (BECN1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1(+)-hSyn-HA-mTREK-1(S131C/A286F)-IRES-GFP

Plasmid

#225490

Purpose

Neuronal expression IRES-containing bicistronic vector for generating mTREK-1 (S131C/A286F) + EGFP under the hSyn promoter.

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Use

Neuronal expression

Tags

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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Kv7.4 (1-645, Δ368-523)/I346A/HA tag/pcDNA3.1

Plasmid

#111460

Purpose

Electrophysiology in HEK cell

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)

Use

Tags

Ha Tag

Expression

Mammalian

Mutation

Kv7.4 (1-645, Δ368-523)/I346A, C643A

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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Kv7.4 (1-645, Δ368-523)/I346D/HA tag/pcDNA3.1

Plasmid

#111461

Purpose

Electrophysiology in HEK cell

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)

Use

Tags

Ha Tag

Expression

Mammalian

Mutation

Kv7.4 (1-645, Δ368-523)/I346D, C643A

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 RL3Sc

Plasmid

#109365

Purpose

Human rod opsin chimera with intracellular loop 3 from Scallop opsin with 1D4 tag

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

Rod opsin chimera with Scallop Opsin2 Loop 3 (RHO, LOC110451845 Patinopecten yessoensis)

Use

Tags

1D4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Rog

Plasmid

#24429

Depositor

Bruce Conklin

Article

Scearce-Levie et al BMC Biol. 2005 . 3():3.

Insert

RASSL (OPRK1 Human)

Use

Tags

FLAG and GFP

Expression

Mammalian

Mutation

mutations in extracellular loops 2 (see comments …

Promoter

Available since

June 16, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3 GATA1 K137R

Plasmid

#85694

Purpose

Mammalian expression of mouse GATA1 with K137R mutation

Depositor

Giannino Del Sal, Licio Collavin

Article

Collavin et al Proc Natl Acad Sci U S A. 2004 Jun 15;10

Insert

GATA1 (Gata1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

K137R (SUMO-1 attachment site)

Promoter

CMV

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only
cacna2d1 BBS pcDNA3

Plasmid

#206106

Purpose

expression of rat alpha2delta-1 calcium channel auxillary subunit with a single Bungarotoxin Binding Site motif inserted after aa549HA

Depositor

Annette Dolphin

Article

Meyer et al Sci Rep. 2021 May 13;11(1):10256. doi: 1

Insert

cacna2d1 (Cacna2d1 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3 hML23Cm6

Plasmid

#109368

Purpose

Human melanopsin chimera with intracellular loops 2-3 and C-terminus from mGluR6

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

human Melanopsin-mGluR6 chimera (OPN4, GRM6 Human)

Use

Tags

1D4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-hL1

Plasmid

#89411

Purpose

mammalian expression of hL1

Depositor

Fritz Rathjen

Article

De Angelis et al EMBO J. 1999 Sep 1. 18(17):4744-53.

Insert

L1CAM (L1CAM Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3 RL23Cm6

Plasmid

#109367

Purpose

Human rod opsin chimera with intracellular loops 2-3 and C-terminus from mGluR6

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

Rod opsin-mGluR6 chimera (RHO, GRM6 Human)

Use

Tags

1D4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)mGAT1YFP*

Plasmid

#41665

Depositor

Henry Lester

Article

Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

Insert

Mus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)

Use

Tags

YFP

Expression

Mammalian

Mutation

“Monomerizing” YFP A206K mutation; C-terminal hyd…

Promoter

CMV

Available since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

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