Skip to main content
Addgene
Showing: 521 - 560 of 2899 results

  1. pSico

    Plasmid
    #11578
    Purpose
    Cre-regulated lentiviral shRNA vector. Cre addition causes EGFP to be recombined out of the construct, activating shRNA expression.
    Depositor
    Tyler Jacks
    Article
    Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Cre/Lox, Lentiviral, and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 19, 2006
    Availability
    Academic Institutions and Nonprofits only
    11578-plasmid-map-sequence-id-106042

    Enlarge

  4. pQadd1R

    Plasmid
    #135661
    Purpose
    Clostridial vector, encoding a targetron carrying two lox511/71 and loxFAS/66 sites in tandem, in the reverse (antisense) orientation. Retarget intron to insert double-lox site at desired location.
    Depositor
    Andrew Tolonen
    Article
    Cerisy et al mSphere. 2019 Dec 11;4(6). pii: 4/6/e007
    Insert
    Intron containing lox site
    Use
    Cre/Lox
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pFer
    Available since
    July 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    135661-plasmid-map-sequence-id-305685

    Enlarge

  5. EC71174

    Plasmid
    #154067
    Purpose
    Level 2 Golden Gate vector, pL2M-HvHSP17-CREU5-UBQ-loxmCHERRYER-eGFPER
    Depositor
    Cristobal Uauy
    Article
    Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:
    Inserts
    HS Cre recombinase
    Ubiquitin promoter driving mCherry with the 35S terminator, flanked by lox sites, followed by eGFP with the Actin terminator
    HYGROMYCIN Resistance Cassette
    Use
    Cre/Lox and Synthetic Biology; Golden gate
    Tags
    Expression
    Bacterial and Plant
    Mutation
    The Arabidopsis thaliana U5 small nuclear ribonuc…
    Promoter
    35S promoter, Hordeum vulgare HSP 17 promoter use…
    Available since
    Sept. 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    154067-plasmid-map-sequence-id-313091

    Enlarge

  6. PB CAG-iCre-ERT2

    Plasmid
    #137858
    Purpose
    CAG-iCre-ERT2 piggyBac transposon
    Depositor
    Mario Capecchi
    Insert
    iCre-ERT2
    Use
    Cre/Lox; Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    137858-plasmid-map-sequence-id-316182

    Enlarge

  7. pPGK-Cre-bpA

    Plasmid
    #11543
    Purpose
    Expresses Cre recombinase from mouse PGK promoter in mammalian cells.
    Depositor
    Klaus Rajewsky
    Insert
    Phosphoglycerate kinase 1 promoter
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    mouse PGK
    Available since
    May 25, 2006
    Availability
    Academic Institutions and Nonprofits only
    11543-plasmid-map-sequence-id-6971

    Enlarge

  9. pSicoR

    Plasmid
    #11579
    Purpose
    Cre-regulated lentiviral shRNA vector. Cre addition causes both EGFP and shRNA to be recombined out of the construct, turning OFF shRNA expression.
    Depositor
    Tyler Jacks
    Article
    Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Cre/Lox, Lentiviral, and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    11579-plasmid-map-sequence-id-251845

    Enlarge

  10. pSico PGK puro

    Plasmid
    #11586
    Purpose
    Cre-regulated lentiviral shRNA vector. Cre addition causes the puromycin gene to be recombined out of the construct, activating shRNA expression.
    Depositor
    Tyler Jacks
    Article
    Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1
    Type
    Empty backbone
    Use
    Cre/Lox, Lentiviral, and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 19, 2006
    Availability
    Academic Institutions and Nonprofits only
    11586-plasmid-map-sequence-id-204172

    Enlarge

  12. pSicoR PGK puro

    Plasmid
    #12084
    Purpose
    Cre-regulated lentiviral shRNA vector. Cre addition causes both puromycin and shRNA to be recombined out of the construct, turning OFF shRNA expression.
    Depositor
    Tyler Jacks
    Article
    Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1
    Type
    Empty backbone
    Use
    Cre/Lox, Lentiviral, and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    12084-plasmid-map-sequence-id-169379

    Enlarge

  13. pQadd1F

    Plasmid
    #135660
    Purpose
    Clostridial vector, encoding a targetron carrying two lox511/71 and loxFAS/66 sites in tandem in the forward (sense) orientation. Retarget intron to insert double-lox site onto genome at desired site.
    Depositor
    Andrew Tolonen
    Article
    Cerisy et al mSphere. 2019 Dec 11;4(6). pii: 4/6/e007
    Insert
    Intron containing lox site
    Use
    Cre/Lox
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pFer
    Available since
    July 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    135660-plasmid-map-sequence-id-305687

    Enlarge

  14. PB CAG-mTagBFP2-T2A-iCreERT2

    Plasmid
    #177308
    Purpose
    CAG-mTagBFP2-T2A-iCre-ERT2 piggyBac transposon
    Depositor
    Mario Capecchi
    Insert
    mTagBFP2-T2A-iCreERT2
    Use
    Cre/Lox; Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    177308-plasmid-map-sequence-id-348855

    Enlarge

  15. PB CAG-sfGFP-T2A-iCreERT2

    Plasmid
    #177309
    Purpose
    CAG-sfGFP-T2A-iCre-ERT2 piggyBac transposon
    Depositor
    Mario Capecchi
    Insert
    sfGFP-T2A-iCreERT2
    Use
    Cre/Lox; Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    177309-plasmid-map-sequence-id-370482

    Enlarge

  16. PB CAG-L-3xSV40pA-L-td-sfGFP

    Plasmid
    #133574
    Purpose
    CAG tdGFP cre/lox reporter piggyBac transposon (3x SV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    Tandem dimer superfolder GFP
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    133574-plasmid-map-sequence-id-274064

    Enlarge

  17. PB CAG-sfGFP-iCre-ERT2

    Plasmid
    #137856
    Purpose
    CAG-sfGFP-iCre-ERT2 piggyBac transposon
    Depositor
    Mario Capecchi
    Insert
    sfGFP-iCre-ERT2
    Use
    Cre/Lox; Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    137856-plasmid-map-sequence-id-316180

    Enlarge

  18. pCAGGS-mTagBFP2-T2A-iCreERT2 (PAGSAS)

    Plasmid
    #137864
    Purpose
    Monomeric TagBFP2 and iCre-ERT2 recombinase (PAGSAS N-terminus)
    Depositor
    Mario Capecchi
    Insert
    mTagBFP2-T2A-iCreERT2
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    137864-plasmid-map-sequence-id-274459

    Enlarge

  19. pCAGGS-mTagBFP2-T2A-iCreERT2 (PA)

    Plasmid
    #128174
    Purpose
    Monomeric TagBFP2 and iCre-ERT2 recombinase (PA N-terminus)
    Depositor
    Mario Capecchi
    Insert
    mTagBFP2-T2A-iCreERT2
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    128174-plasmid-map-sequence-id-247966

    Enlarge

  20. pCAGGS-mTagBFP2-T2A-iCreERT2 (PV)

    Plasmid
    #128171
    Purpose
    Monomeric TagBFP2 and iCre-ERT2 recombinase (PV N-terminus)
    Depositor
    Mario Capecchi
    Insert
    mTagBFP2-T2A-iCreERT2
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    128171-plasmid-map-sequence-id-247962

    Enlarge

  21. pCAGGS-mTagBFP2-T2A-iCreERT2 (PAA)

    Plasmid
    #137859
    Purpose
    Monomeric TagBFP2 and iCre-ERT2 recombinase (PAA N-terminus)
    Depositor
    Mario Capecchi
    Insert
    mTagBFP2-T2A-iCreERT2
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    137859-plasmid-map-sequence-id-274442

    Enlarge

  22. fabp10a:CreERT2; cryaa:mCerulean

    Plasmid
    #230044
    Purpose
    Inducible Cre expression in Zebrafish hepatocytes
    Depositor
    Sumeet Pal Singh
    Insert
    CreERT2
    Use
    Cre/Lox; Zebrafish expression
    Tags
    Expression
    Mutation
    Promoter
    fabp10a
    Available since
    Feb. 11, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    230044-plasmid-map-sequence-id-461060

    Enlarge

  23. pCAGGS-mTagBFP2-T2A-iCreERT2 (PAAA)

    Plasmid
    #137860
    Purpose
    Monomeric TagBFP2 and iCre-ERT2 recombinase (PAAA N-terminus)
    Depositor
    Mario Capecchi
    Insert
    mTagBFP2-T2A-iCreERT2
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    137860-plasmid-map-sequence-id-274446

    Enlarge

  24. pCAGGS-mTagBFP2-T2A-iCreERT2 (PAAAA)

    Plasmid
    #137861
    Purpose
    Monomeric TagBFP2 and iCre-ERT2 recombinase (PAAAA N-terminus)
    Depositor
    Mario Capecchi
    Insert
    mTagBFP2-T2A-iCreERT2
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    137861-plasmid-map-sequence-id-274449

    Enlarge

  25. pCAGGS-mTagBFP2-T2A-iCreERT2 (PAGSA)

    Plasmid
    #137862
    Purpose
    Monomeric TagBFP2 and iCre-ERT2 recombinase (PAGSA N-terminus)
    Depositor
    Mario Capecchi
    Insert
    mTagBFP2-T2A-iCreERT2
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    137862-plasmid-map-sequence-id-274455

    Enlarge

  26. pCAGGS-mTagBFP2-T2A-iCreERT2 (PAAGSA)

    Plasmid
    #137863
    Purpose
    Monomeric TagBFP2 and iCre-ERT2 recombinase (PAAGSA N-terminus)
    Depositor
    Mario Capecchi
    Insert
    mTagBFP2-T2A-iCreERT2
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    137863-plasmid-map-sequence-id-274456

    Enlarge

  27. pCAGGS-mTagBFP2-T2A-iCreERT2 (PVV)

    Plasmid
    #128172
    Purpose
    Monomeric TagBFP2 and iCre-ERT2 recombinase (PVV N-terminus)
    Depositor
    Mario Capecchi
    Insert
    mTagBFP2-T2A-iCreERT2
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    128172-plasmid-map-sequence-id-247964

    Enlarge

  28. pCAGGS-mTagBFP2-T2A-iCreERT2 (PVGSA)

    Plasmid
    #128173
    Purpose
    Monomeric TagBFP2 and iCre-ERT2 recombinase (PVGSA N-terminus)
    Depositor
    Mario Capecchi
    Insert
    mTagBFP2-T2A-iCreERT2
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    128173-plasmid-map-sequence-id-254324

    Enlarge

  31. IRES-creERT2-FNF

    Plasmid
    #188230
    Purpose
    EMCV IRES-cre-ERT2-FRT-Neo*-FRT cassette
    Depositor
    Mario Capecchi
    Insert
    IRES-creERT2-FNF
    Use
    Cre/Lox and Mouse Targeting
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Sept. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    188230-plasmid-map-sequence-id-371215

    Enlarge

  32. CMV-H2B-sfGFP-T2A-iCre

    Plasmid
    #183351
    Purpose
    Histone H2B-sfGFP and iCre recombinase
    Depositor
    Mario Capecchi
    Insert
    Histone H2B-sfGFP-T2A-iCre
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    183351-plasmid-map-sequence-id-360158

    Enlarge

  33. PB CAG-L-bpA-3xSV40pA-L-td-sfGFP

    Plasmid
    #133577
    Purpose
    CAG tdGFP cre/lox reporter piggyBac transposon (bpA 3x SV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    Tandem dimer superfolder GFP
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    133577-plasmid-map-sequence-id-274079

    Enlarge

  34. PB CAG-L-bpA-2xSV40pA-L-td-sfGFP

    Plasmid
    #133576
    Purpose
    CAG tdGFP cre/lox reporter piggyBac transposon (bpA 2x SV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    Tandem dimer superfolder GFP
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    133576-plasmid-map-sequence-id-274071

    Enlarge

  35. PB CAG-L-bpA-SV40pA-L-td-sfGFP

    Plasmid
    #133575
    Purpose
    CAG tdGFP cre/lox reporter piggyBac transposon (bpA 1x SV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    Tandem dimer superfolder GFP
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    133575-plasmid-map-sequence-id-274068

    Enlarge

  36. cTRE-LSL-GFP-IRES-Cas9-sgCR8

    Plasmid
    #135668
    Purpose
    Introduce Dox/Cre-inducible (TRE promoter followed by lox-stop-lox) Cas9 cDNA plus control-targeting sgRNA into (ES) cells by recombination-mediated cassette exchange
    Depositor
    Florian Karreth
    Insert
    sgCR8
    Use
    Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    135668-plasmid-map-sequence-id-325838

    Enlarge

  37. cTRE-LSL-GFP-IRES-Cas9-sgPtenX1.1

    Plasmid
    #135669
    Purpose
    Introduce Dox/Cre-inducible (TRE promoter followed by lox-stop-lox) Cas9 cDNA plus Pten-targeting sgRNA into (ES) cells by recombination-mediated cassette exchange
    Depositor
    Florian Karreth
    Insert
    sgPten
    Use
    Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    135669-plasmid-map-sequence-id-325841

    Enlarge

  38. PB CAG-sfGFP-GSAx9-iCre-ERT2

    Plasmid
    #137857
    Purpose
    CAG-sfGFP-GSAx9-iCre-ERT2 piggyBac transposon
    Depositor
    Mario Capecchi
    Insert
    sfGFP-GSAx9-iCre-ERT2
    Use
    Cre/Lox; Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    137857-plasmid-map-sequence-id-316181

    Enlarge

  39. PB CAG-L-bpA-2xSV40pA-L-tdGFP-Pten_shRNAmir

    Plasmid
    #178044
    Purpose
    CAG tdGFP Pten shRNAmir cre/lox expression piggyBac transposon (bpA 2xSV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    Pten_1523 shRNAmir
    Use
    Cre/Lox and RNAi; Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    178044-plasmid-map-sequence-id-352916

    Enlarge

  40. PB CAG-L-bpA-2xSV40pA-L-tdGFP-Renilla_shRNAmir

    Plasmid
    #178046
    Purpose
    CAG tdGFP Renilla shRNAmir cre/lox expression piggyBac transposon (bpA 2xSV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    Renilla_713 shRNAmir
    Use
    Cre/Lox and RNAi; Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    178046-plasmid-map-sequence-id-352920

    Enlarge

Showing: 521 - 560 of 2899 results