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pB[U6-3 wex2 PUb-DsRed]

Plasmid

#169010

Purpose

For germline transformation. Expresses white exon 2 sgRNA and DsRed marker in all cells.

Depositor

Max Scott

Article

Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

Insert

white ex2 sgRNA

Use

Piggybac

Tags

Expression

Insect

Mutation

Promoter

Drosophila melanogaster U6:3

Available since

April 11, 2022

Availability

Academic Institutions and Nonprofits only

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pUASp-GBP-Tbro

Plasmid

#179995

Purpose

Expresses two tandem copies of the GFP nanobody fused at the C-terminus with TurboID. Expression is driven using the UAS-Gal4 system. The UASp version is ideal for expression in the germline.

Depositor

Graydon Gonsalvez

Article

Baker et al Development. 2021 Nov 15;148(22). pii: 2

Insert

GBP nanobody

Use

Tags

Trbo

Expression

Insect

Mutation

Promoter

UAS-Gal4

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pSEM415 - mosTI unc-119 Pmex-5 - gfp(nls) - tbb-2

Plasmid

#182358

Purpose

Positive control for mosTI(unc-119). unc-119 rescue and germline nuclear GFP expression after mosTI insertion.

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

GFP

Use

Tags

Expression

Worm

Mutation

Promoter

Pmex-5

Available since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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pSEM391 - mosTI unc-119 - Pmex-5 - NNN - tbb-2

Plasmid

#182351

Purpose

Germline expression cloning vector for mosTI(unc-119). Transgene inserted between Pmex-5 and tbb-2 3'UTR. MCS or Golden Gate (BsaI: aaaa - MCS - tgag)

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Type

Empty backbone

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

May 13, 2022

Availability

Academic Institutions and Nonprofits only

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pCFJ150-Cas9(dpiRNA)

Plasmid

#107940

Purpose

eft-3p::Cas9(dpiRNA)::tbb-2 3'UTR construct used for MosSCI in nematode. Cas9 is optimized by removing all piRNA targeting sites to allow germline expression.

Depositor

Heng-Chi Lee

Article

Zhang et al Science. 2018 Feb 2;359(6375):587-592. d

Insert

Cas9(dpiRNA)

Use

Tags

Expression

Worm

Mutation

Promoter

eft-3

Available since

April 4, 2018

Availability

Academic Institutions and Nonprofits only

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pCFJ150-mCherry(dpiRNA)::ANI-1(AHPH)

Plasmid

#107939

Purpose

pie-1p::mCherry(dpiRNA)::ANI-1(AHPH)::pie-1 3'UTR construct used for MosSCI in nematode. mCherry is optimized by removing all piRNA targeting sites to allow germline expression. RhoA biosenser.

Depositor

Heng-Chi Lee

Article

Zhang et al Science. 2018 Feb 2;359(6375):587-592. d

Insert

mCherry(dpiRNA)::ANI-1(AHPH)

Use

Tags

Expression

Worm

Mutation

Promoter

pie-1

Available since

April 2, 2018

Availability

Academic Institutions and Nonprofits only

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pCFJ150-GFP(dpiRNA)::CDK-1

Plasmid

#107938

Purpose

cdk-1p::GFP(dpiRNA)::cdk-1::cdk-1 3'UTR construct used for MosSCI in nematode. GFP is optimized by removing all piRNA targeting sites to allow germline expression.

Depositor

Heng-Chi Lee

Article

Zhang et al Science. 2018 Feb 2;359(6375):587-592. d

Insert

GFP(dpiRNA)::CDK-1 (cdk-1 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

cdk-1

Available since

April 4, 2018

Availability

Academic Institutions and Nonprofits only

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pLVX-IRES-MRGPRB2-mCherry

Plasmid

#213961

Purpose

Expresses MRGPRB2 in mammalian cells

Depositor

Christa Müller

Article

Al Hamwi et al Commun Biol. 2024 Jan 6;7(1):52. doi: 10

Insert

MRGPRB2 (Mrgprb2 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 14, 2024

Availability

Academic Institutions and Nonprofits only

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pUASp-FMW-attB-AthHen1

Plasmid

#104957

Purpose

Expression of FLAG Myc tagged A.thaliana-Hen1 (codon optimised for Drosophila) in Drosophila germline tissues using Gal4 drivers

Depositor

Stefan Ameres

Article

Alberti et al Nat Methods. 2018 Feb 26. pii: nmeth.461

Insert

Hen1 (HEN1 Mustard Weed)

Use

Tags

FLAG Myc

Expression

Insect

Mutation

Promoter

pTrasposase promoter

Available since

Jan. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pcs2+spCas9 ABE-Umax-nanos

Plasmid

#222142

Purpose

Adenine base editor with modified TadA variant for germline A-to-G base editing in zebrafish

Depositor

Gaurav Varshney

Article

Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

Insert

Modified TadA-nSpCas9 with nanos UTR

Use

CRISPR; Zebrafish expression

Tags

Bipartite NLS

Expression

Mutation

Promoter

SP6

Available since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pX330_gRNA_IG-DMR-1

Plasmid

#159931

Purpose

Specific gRNA against mouse IG-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of IG-DMR in mouse ESC.

Depositor

Anton Wutz

Article

Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

Insert

gRNA mouse IG-DMR

Use

CRISPR and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

U6, CBh

Available since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pX330_gRNA_IG-DMR-2

Plasmid

#159932

Purpose

Specific gRNA against mouse IG-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of IG-DMR in mouse ESC.

Depositor

Anton Wutz

Article

Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

Insert

gRNA mouse IG-DMR

Use

CRISPR and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

U6, CBh

Available since

Nov. 4, 2020

Availability

Academic Institutions and Nonprofits only

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phCMV-GALV-MTR

Plasmid

#163612

Purpose

Retro and Lentiviral transduction of primary human germinal center B cells

Depositor

Daniel Hodson

Article

Caeser et al Nat Commun. 2019 Oct 4;10(1):4543. doi:

Insert

GaLV MTR envelope

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pBpaRS3tRNA

Plasmid

#197102

Purpose

To express the p-benzoylphenylalanine-specific variant of Methanocaldococcus jannaschii TyrRS and its cognate amber suppressor tRNA and allow UAG codon to be translated into p-benzoylphenylalanine.

Depositor

Kensaku Sakamoto

Article

Sato et al Biochemistry. 2011 Jan 18;50(2):250-7. d

Insert

TyrRS variant, amber suppressor tRNA

Use

Tags

Expression

Bacterial

Mutation

Gly32, Ser107, Thr158, Ser159, Arg286 (TyrRS)

Promoter

Available since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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