We narrowed to 10,731 results for: Kars

1. miniTol2 x EF1a_EKAREN4 + PGK-blast

   Plasmid

   #167825

   Purpose

   Optimized EKAREV FRET biosensor sensor for ERK

   Depositor

   Hugo Snippert

   Article

   Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

   Insert

   EKAREN4
   

   Use

   Tags

   nls localization motif

   Expression

   Mammalian

   Mutation

   K424P, K426W

   Promoter

   EF1a

   Available since

   April 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-HaloCKAR2.2-T2A-EGFP

   Plasmid

   #216493

   Purpose

   Expression of chemigenetic PKC biosensor (low basal brightness, extremly high dynamic range) and EGFP transfection marker in mammalian cells

   Depositor

   Jin Zhang

   Article

   Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

   Insert

   HaloCKAR2.2
   

   Use

   Tags

   T2A-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-HaloAktKAR2.2-T2A-EGFP

   Plasmid

   #216494

   Purpose

   Expression of chemigenetic Akt biosensor (low basal brightness, extremly high dynamic range) and EGFP transfection marker in mammalian cells

   Depositor

   Jin Zhang

   Article

   Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

   Insert

   HaloAktKAR2.2
   

   Use

   Tags

   T2A-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-HaloAKAR2.0-T2A-EGFP

   Plasmid

   #216488

   Purpose

   Expression of chemigenetic PKA biosensor (high basal brightness, medium dynamic range) and EGFP transfection marker in mammalian cells

   Depositor

   Jin Zhang

   Article

   Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

   Insert

   HaloAKAR2.0
   

   Use

   Tags

   T2A-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-HaloAKAR2.1-T2A-EGFP

   Plasmid

   #216489

   Purpose

   Expression of chemigenetic PKA biosensor (medium basal brightness, high dynamic range) and EGFP transfection marker in mammalian cells

   Depositor

   Jin Zhang

   Article

   Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

   Insert

   HaloAKAR2.1
   

   Use

   Tags

   T2A-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-Lyso-ExRai-CKAR2

   Plasmid

   #236100

   Purpose

   Enhanced excitation-ratiometric biosensor for monitoring Protein Kinase C activity in living cells, targeted to lysosome surface.

   Depositor

   Jin Zhang

   Article

   Su et al Nat Chem Biol. 2024 Oct 11. doi: 10.1038

   Insert

   LAMP1-ExRai-CKAR2 (LAMP1 Human, Synthetic)
   

   Use

   Tags

   Full-length LAMP1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-Par6-ExRai-CKAR2

   Plasmid

   #236101

   Purpose

   Subcellularly targeted enhanced excitation-ratiometric biosensor for monitoring Protein Kinase C activity in living cells for selective detection of aPKC activity within the Par complex.

   Depositor

   Jin Zhang

   Article

   Su et al Nat Chem Biol. 2024 Oct 11. doi: 10.1038

   Insert

   Par6C-ExRai-CKAR2 (PARD6A Human, Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-Cyto-ExRai-CKAR2

   Plasmid

   #236097

   Purpose

   Cytosol-targeted enhanced excitation-ratiometric biosensor for monitoring Protein Kinase C activity in living cells.

   Depositor

   Jin Zhang

   Article

   Su et al Nat Chem Biol. 2024 Oct 11. doi: 10.1038

   Insert

   ExRai-CKAR2-NES
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. YIplac211-Kar2-moxGFP2-HDEL

   Plasmid

   #218970

   Purpose

   Gene replacement plasmid to label S. cerevisiae Kar2 with moxGFP2

   Depositor

   Benjamin Glick

   Insert

   KAR2-moxGFP2-HDEL (KAR2 Budding Yeast)
   

   Use

   Gene replacement vector for yeast

   Tags

   moxGFP2

   Expression

   Mutation

   Promoter

   Available since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pnYC-NpM-DmOskar-PC_Q

    Plasmid

    #147323

    Purpose

    Bacterial Expression of DmOskar-PC

    Depositor

    Elisa Izaurralde

    Insert

    DmOskar-PC (osk Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1(+)-FluoSTEP-AKAR (mScarlet-I)

    Plasmid

    #181861

    Purpose

    FluoSTEP-AKAR using mScarlet-I as the FRET acceptor.

    Depositor

    Jin Zhang

    Article

    Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

    Insert

    FluoSTEP-AKAR (mScarlet-I)
    

    Use

    Tags

    mScarlet-I and sfGFP(1-10)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAc5.1C-Fluc-oskar-SRE1mut_C

    Plasmid

    #146023

    Purpose

    Insect Expression of oskar-3UTR-SRE1mut

    Depositor

    Elisa Izaurralde

    Insert

    oskar-3UTR-SRE1mut (osk Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV x EF1a_EKAREN4(TA)-ires-blast

    Plasmid

    #167831

    Purpose

    Control sensor for EKAREN4

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN4(TA)
    

    Use

    Lentiviral

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W, T420A

    Promoter

    EF1a

    Available since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. YIplac211-KAR2-mEGFP-HDEL

    Plasmid

    #160467

    Purpose

    Yeast integration vector for the gene replacement of S. cerevisiae KAR2 fused to mEGFP with an ER retrieval signal

    Depositor

    Benjamin Glick

    Article

    Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

    Insert

    KAR2-mEGFP-HDEL
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMSCV-FLAG-hIKAROS-IRES-GFP

    Plasmid

    #74046

    Purpose

    retroviral plasmid for expression of FLAG-tagged human Ikaros corresponding to cDNA from XM_011515058.1

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    human Ikaros (IKZF1) (IKZF1 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    pMSCV-LTRs

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-mCherry-mCherry-FLARE-AKAR

    Plasmid

    #123333

    Purpose

    Red-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    mCherry-mCherry-FLARE-AKAR
    

    Use

    Tags

    6xHIS, T7 tag (gene 10 leader), Xpress (TM) tag, …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-Venus-cpVenus-FLARE-AKAR

    Plasmid

    #123329

    Purpose

    Yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity. Contains monomeric Venus/cpVenus-E172 homoFRET pair.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    Venus-cpVenus-FLARE-AKAR
    

    Use

    Tags

    6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3-Venus-cpVenus-FLARE-AKAR(T/A)

    Plasmid

    #123330

    Purpose

    Negative control mutant for Venus-cpVenus-FLARE-AKAR biosensor.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    Venus-cpVenus-FLARE-AKAR(T/A)
    

    Use

    Tags

    6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

    Expression

    Mammalian

    Mutation

    Target Thr residue in substrate domain mutated to…

    Promoter

    CMV

    Available since

    July 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p426MET25_SSKar2-sfpHluorin-HDEL (ER-sfpHluorin, MRV66)

    Plasmid

    #115698

    Purpose

    ER-targeted superfolder pHluorin for improved pH measurements in the ER of S. cerevisiae

    Depositor

    Eckhard Boles

    Article

    Reifenrath et al Sci Rep. 2018 Aug 10;8(1):11985. doi: 10

    Insert

    SSKar2-sfpHluorin-HDEL
    

    Use

    Tags

    ER retention signal containing HDEL and ER signal…

    Expression

    Yeast

    Mutation

    for introduced mutations see publication

    Promoter

    MET25

    Available since

    Sept. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p7133 pHAGE-P-CMVt-N-HA-GAW-PRKAR2B

    Plasmid

    #100160

    Purpose

    Expresses PRKAR2B

    Depositor

    Peter Howley

    Article

    Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

    Insert

    PRKAR2B (PRKAR2B Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge