We narrowed to 8,979 results for: Pol

1. Venus-tagged Apollo-NADP+

   Plasmid

   #71797

   Purpose

   Expresses Venus-tagged Apollo-NADP+ in mammalian cells as a reporter for cytoplasmic NADP+

   Depositor

   Jonathan Rocheleau

   Article

   Cameron et al Nat Methods. 2016 Feb 15. doi: 10.1038/n

   Insert

   Apollo-NADP+
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. Cerulean-tagged Apollo-NADP+

   Plasmid

   #71800

   Purpose

   Expresses Cerulean-tagged Apollo-NADP+ in mammalian cells as a reporter for cytoplasmic NADP+

   Depositor

   Jonathan Rocheleau

   Article

   Cameron et al Nat Methods. 2016 Feb 15. doi: 10.1038/n

   Insert

   Apollo-NADP+
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. AP1muA-TurboID-polyA-G418 HR

   Plasmid

   #227734

   Purpose

   Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with TurboID and a V5 epitope tag. Contains a G418 resistance cassette for selection of edited cells.

   Depositor

   Francesca Bottanelli

   Article

   Stockhammer et al J Cell Sci. 2024 Aug 15;137(16):jcs26195

   Insert

   genomic sequence AP1M1 locus (AP1M1 Human)
   

   Use

   CRISPR

   Tags

   Turbo-ID, V5

   Expression

   Mutation

   Promoter

   Available since

   Jan. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV uN2CpolyGly op GFP (NIID)

   Plasmid

   #224356

   Purpose

   Express GFP tagged upsteram ORF of NOTCH2NLC with an expansion (100x) of GGC repeats with codon optimization (NOT pure GGC repeats)

   Depositor

   Nicolas Charlet-Berguerand

   Article

   Boivin et al Neuron. 2021 Jun 2;109(11):1825-1835.e5.

   Insert

   uN2C with a GGC repeat expansion (100x)
   

   Use

   AAV

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Codon-optimized for expression in human, so no pu…

   Promoter

   CMV + chimeric intron (CAG)

   Available since

   Feb. 5, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pMXS-IRES-Blast POLE1

   Plasmid

   #160802

   Purpose

   Express POLE1

   Depositor

   Richard Possemato

   Article

   Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

   Insert

   POLE1 (POLE Human)
   

   Use

   Retroviral

   Tags

   3xFLAG

   Expression

   Mutation

   shPOLE_1 and shPOLE2 resistant

   Promoter

   Available since

   Jan. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLL3.7-EF-EYFP-YAP1_WT-PolyA

   Plasmid

   #112284

   Purpose

   Fluorescently labels WT human YAP1-2gamma isoform (504 aa)

   Depositor

   Erik Sahai

   Article

   Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

   Insert

   EYFP-YAP1 (YAP1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   WT human YAP1-2gamma

   Promoter

   EF-1 alpha

   Available since

   May 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV.GFAP.SV40.Thbs1-HA.SV40(polyA)

   Plasmid

   #195552

   Purpose

   Expresses Thbs1 under control of short GFAP promoter

   Depositor

   Kevin Park

   Article

   Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

   Insert

   Thrombospondin-1 (Thbs1 Mouse)
   

   Use

   AAV

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   Promoter

   short GFAP

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. hSyn-DIO-somBiPOLES-eGFP

   Plasmid

   #192584

   Purpose

   AAV-vector for bidirectional optogenetic manipulation of Cre-positive neurons

   Depositor

   Simon Wiegert

   Insert

   somBiPOLES
   

   Use

   AAV

   Tags

   soma-targeting motif from Kv2.1 channel

   Expression

   Mammalian

   Mutation

   Promoter

   human synapsin

   Available since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMXS-IRES-Blast HA-POLE2

   Plasmid

   #160809

   Purpose

   Express HA-tagged POLE2

   Depositor

   Richard Possemato

   Article

   Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

   Insert

   HA-POLE2 (POLE2 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mutation

   Promoter

   Available since

   Oct. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. Perox-mTurq2-Apollo-NADP+

    Plasmid

    #193304

    Purpose

    Expresses mTurq2-Apollo-NADP+ sensor localized to the peroxisome

    Depositor

    Jonathan Rocheleau

    Article

    Chang et al ACS Sens. 2022 Oct 21. doi: 10.1021/acss

    Insert

    G6PD (G6PD Human)
    

    Use

    Tags

    mTurqoise2, peroxisomal targeting sequence

    Expression

    Mammalian

    Mutation

    S40A, R72Q, H201N, K205T

    Promoter

    T7

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. mAC-POLR2A donor (Hygro)

    Plasmid

    #124496

    Purpose

    A donor plasmid for tagging the N-terminus of human POLR2A with mAID-mClover

    Depositor

    Masato Kanemaki

    Article

    Nagashima et al J Cell Biol. 2019 Mar 1. pii: jcb.201811

    Insert

    POLR2A homology arms and Hygro-P2A-mAID-mClover (POLR2A Human)
    

    Use

    CRISPR

    Tags

    mAID-mClover

    Expression

    Mutation

    Promoter

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLL3.7-EF-EYFP-YAP1_S94A-PolyA

    Plasmid

    #112286

    Purpose

    Fluorescently labels human YAP1-2gamma isoform (504 aa) S94A mutant

    Depositor

    Erik Sahai

    Article

    Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

    Insert

    EYFP-YAP1 (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Human YAP1-2gamma with Serine 94 to Alanine

    Promoter

    EF-1 alpha

    Available since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. AP1muA-SNAP-V5-polyA-Puro HR

    Plasmid

    #229680

    Purpose

    Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with SNAP tag and a V5 epitope tag. Contains a puromycin resistance cassette for selection of edited cells.

    Depositor

    Francesca Bottanelli

    Article

    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

    Insert

    genomic sequence AP1M1 locus (AP1M1 Human)
    

    Use

    CRISPR

    Tags

    SNAP, V5

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV.CMV.SV40.THBS4-HA.SV40(polyA)

    Plasmid

    #155193

    Purpose

    Expresses murine Thrombospondin-4 (THBS4) protein with HA-tag at C-terminus

    Depositor

    Kevin Park

    Article

    Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

    Insert

    Thrombospondin-4 (Thbs4 Mouse)
    

    Use

    AAV

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. p3E-U6a-polg guide

    Plasmid

    #107598

    Purpose

    An entry vector with U6a promoter driving polg guide RNA expression

    Depositor

    Qing Deng

    Article

    Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

    Insert

    polg guide RNA sequence
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. Wild-type AdPol vector

    Plasmid

    #131685

    Purpose

    A lentiviral vector that constitutively expresses the wild-type adenovirus DNA polymerase

    Depositor

    Matthew D. Shoulders

    Article

    Berman et al J Am Chem Soc. 2018 Dec 26;140(51):18093

    Insert

    Adenovirus DNA polymerase
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA-flag-mKdm4d-polyA

    Plasmid

    #61553

    Purpose

    For mRNA synthesis or expression in mammalian cells

    Depositor

    Yi Zhang

    Article

    Matoba et al Cell. 2014 Nov 6;159(4):884-95. doi: 10.

    Insert

    Kdm4d (Kdm4d Mouse)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Feb. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. AICSDP-117:POLR2A-mEGFP

    Plasmid

    #164500

    Purpose

    Homology arms and mEGFP-linker sequence sequence for N-terminus tagging of human POLR2A

    Depositor

    Allen Institute for Cell Science

    Insert

    POLR2A Homology Arms with mEGFP-linker (POLR2A Human)
    

    Use

    CRISPR; Donor template

    Tags

    mEGFP-linker

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available since

    Feb. 17, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. Renilla luciferase-Pol III

    Plasmid

    #37380

    Depositor

    Norbert Perrimon

    Article

    Nybakken et al Nat Genet. 2005 Dec;37(12):1323-32. Epub

    Insert

    PolIII-Renilla control reporter (RpIII128 Fly)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    RNA PolIII 128 subunit (RpIII128)

    Available since

    July 30, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA-RanWT-mRFP1-polyA

    Plasmid

    #59750

    Purpose

    Express wild-type form of Ran-mRFP1 in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    Ran-mRFP1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Sept. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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