Addgene

We narrowed to 10,960 results for: Vars;

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pCR3.1-OPNb

Plasmid

#107981

Purpose

expression of OPN splice variant b

Depositor

Georg Weber

Article

He et al Oncogene. 2006 Apr 6;25(15):2192-202. do

Insert

osteopontin splice variant b (SPP1 Human)

Use

Tags

Expression

Mammalian

Mutation

splice variant

Promoter

Available since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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pSEVA451-Cpf1

Plasmid

#192362

Purpose

CRISPR-based vector for genome editing

Depositor

Juana María Navarro Llorens

Article

Baldanta et al Microb Cell Fact. 2022 May 28;21(1):103.

Insert

Cas12a, gRNA scaffold

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pSEVA351-Cpf1

Plasmid

#192361

Purpose

CRISPR-based vector for genome editing

Depositor

Juana María Navarro Llorens

Article

Baldanta et al Microb Cell Fact. 2022 May 28;21(1):103.

Insert

Cas12a, gRNA scaffold

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-Miro1v4

Plasmid

#174111

Purpose

Expresses the peroxisomal variant (splice variant 4) of Miro with an N-terminal EGFP.

Depositor

Pietro De Camilli

Article

Guillen-Samander et al J Cell Biol. 2021 May 3;220(5). pii: 212

Insert

Miro1 (var4, peroxisomal splice variant)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pWSK29-wzzBST

Plasmid

#172968

Purpose

pWSK29 with Salmonella typhimurium wzzBST gene

Depositor

Renato Morona

Article

Murray et al J Bacteriol. 2006 Apr;188(7):2735-9. doi

Insert

wzzB gene from Salmonella enterica serovar Typhimurium

Use

Tags

Expression

Bacterial

Mutation

K198E- please see depositor comments

Promoter

Available since

Aug. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pWSK29-fepEST

Plasmid

#172969

Purpose

pWSK29 with Salmonella typhimurium fepEST gene

Depositor

Renato Morona

Article

Murray et al J Bacteriol. 2006 Apr;188(7):2735-9. doi

Insert

fepE gene from Salmonella enterica serovar Typhimurium

Use

Tags

Expression

Bacterial

Mutation

G160S- please see depositor comments

Promoter

Available since

Aug. 26, 2021

Availability

Academic Institutions and Nonprofits only

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2Flag-pDEST-N

Plasmid

#118371

Purpose

Gateway compatible mammalian expression vector with N-terminal 2Flag to tag proteins of interest.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Type

Empty backbone

Use

Destination

Tags

2Flag

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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2Flag-pDEST-C

Plasmid

#118372

Purpose

Gateway compatible mammalian expression vector with C-terminal 2Flag to tag proteins of interest.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Type

Empty backbone

Use

Destination

Tags

2Flag

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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HA-GFP1-10-pDEST-C

Plasmid

#118369

Purpose

Bimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible C-terminal vector with BiFC-tag (HA-GFP1-10) to screen interaction partners.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Type

Empty backbone

Use

Destination

Tags

HA-GFP1-10

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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HA-GFP1-10-pDEST-N

Plasmid

#118368

Purpose

Bimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible N-terminal vector with BiFC-tag (HA-GFP1-10) to screen interaction partners.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Type

Empty backbone

Use

Destination

Tags

HA-GFP1-10

Expression

Mammalian

Mutation

Promoter

Available since

May 1, 2019

Availability

Academic Institutions and Nonprofits only

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2HA-pDEST-C

Plasmid

#118374

Purpose

Gateway compatible mammalian expression vector with C-terminal 2HA to tag proteins of interest.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Type

Empty backbone

Use

Destination

Tags

2HA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 30, 2018

Availability

Academic Institutions and Nonprofits only

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2HA-pDEST-N

Plasmid

#118373

Purpose

Gateway compatible mammalian expression vector with N-terminal 2HA to tag proteins of interest.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Type

Empty backbone

Use

Destination

Tags

2HA

Expression

Mammalian

Mutation

Promoter

Available since

May 1, 2019

Availability

Academic Institutions and Nonprofits only

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Full-length P229S talin1

Plasmid

#182993

Purpose

express FL variant P229S talin1 in mammalian cells

Depositor

Vesa Hytönen

Article

Azizi et al Hum Mol Genet. 2022 Jul 21. pii: 6647920

Insert

Talin1 P229S (Tln1 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

P229S

Promoter

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_NLE1:1-485

Plasmid

#211141

Purpose

Mammalian expression of full-length human NLE1. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

NLE1:M1-R485 (NLE1 Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

P6A natural variant (VAR_060327). R169K natural…

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_NLE1:1-485

Plasmid

#211182

Purpose

Mammalian expression of full-length human NLE1. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

NLE1:M1-R485 (NLE1 Human)

Use

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

P6A natural variant (VAR_060327). R169K natural v…

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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Flag-GFP11-pDEST-N

Plasmid

#118366

Purpose

Bimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible N-terminal vector with BiFC-tag (GFP11-Flag) to screen interaction partners.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Type

Empty backbone

Use

Destination

Tags

GFP11-Flag

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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Flag-GFP11-pDEST-C

Plasmid

#118367

Purpose

Bimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible C-terminal vector with BiFC-tag (GFP11-Flag) to screen interaction partners.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Type

Empty backbone

Use

Destination

Tags

GFP11-FLAG

Expression

Mammalian

Mutation

Promoter

Available since

May 1, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hLbCpf1(TYCV) (pY230)

Plasmid

#89355

Purpose

Expresses humanized LbCpf1 variant that recognizes TYCV PAMs

Depositor

Feng Zhang

Article

Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

Insert

Cpf1, RR variant

Use

CRISPR

Tags

NLS and NLS-3xHA

Expression

Mammalian

Mutation

G532R/K595R

Promoter

CMV

Available since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hAsCpf1(TATV) (pY220)

Plasmid

#89353

Purpose

Expresses humanized AsCpf1 variant that recognizes TATV PAMs

Depositor

Feng Zhang

Article

Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

Insert

Cpf1, RVR variant

Use

CRISPR

Tags

NLS and NLS-3xHA

Expression

Mammalian

Mutation

S542R/K548V/N552R

Promoter

CMV

Available since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hAsCpf1(TYCV) (pY210)

Plasmid

#89351

Purpose

Expresses humanized AsCpf1 variant that recognizes TYCV PAMs

Depositor

Feng Zhang

Article

Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

Insert

Cpf1, RR variant

Use

CRISPR

Tags

NLS and NLS-3xHA

Expression

Mammalian

Mutation

S542R/K607R

Promoter

CMV

Available since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

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