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pcDNA3.1dsLuc2CP

Plasmid

#68054

Purpose

expresses destabilized Firefly Luciferase

Depositor

Christopher Sullivan

Article

Seo et al J Virol. 2008 Oct;82(20):9823-8. doi: 10

Insert

Firefly luciferase

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only
GtCCR2_EYFP_pcDNA3.1

Plasmid

#159121

Purpose

This plasmid encodes GtCCR2 rhodopsin domain

Depositor

John Spudich

Article

Sineshchekov et al Proc Natl Acad Sci U S A. 2017 Nov 7;114

Insert

GtCCR2

Use

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_rSERT_WT

Plasmid

#190743

Purpose

For expression of wild-type rat SERT in mammalian cells

Depositor

Gary Rudnick

Article

Singh et al bioRxiv 2022.06.13.495991

Insert

SLC6A4 Serotonin Transporter (Slc6a4 Rat)

Use

Tags

FLAG, His6, and c-myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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AlACR1_EYFP_pcDNA3.1

Plasmid

#161022

Purpose

Expresses strongly red-shifted AlACR1 in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Proc Natl Acad Sci U S A. 2020 Sep 15;11

Insert

AlACR1

Use

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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T1ACR1_EYFP_pcDNA3.1

Plasmid

#161027

Purpose

Expresses strongly red-shifted T1ACR1 in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Proc Natl Acad Sci U S A. 2020 Sep 15;11

Insert

T1ACR1

Use

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_SNORD27h_2ntmut

Plasmid

#73067

Purpose

expression clone for human mutated SNORD27 (C/D box snoRNA U27)

Depositor

Stefan Stamm

Article

Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

Insert

SNORD27 (E2F7 Human)

Use

Tags

no tag

Expression

Mammalian

Mutation

2 nt mutation in AS box that binds E2F7 pre-mRNA

Promoter

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 PA-NL

Plasmid

#113445

Purpose

ProteinA-NanoLuc hybrid fusion control expression vector (for BRET and LuC normalization)

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Insert

ProteinA-NanoLuc

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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CRAF_S29A_pcDNA3.1

Plasmid

#86506

Purpose

Expresses CRAF_S29A in mammalian cells [Edit]

Depositor

Dustin Maly

Article

Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

Insert

CRAF (RAF1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

S29A

Promoter

CMV

Available since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_SNORD27h_115mut

Plasmid

#73068

Purpose

expression clone for human mutated SNORD27 (C/D box snoRNA U27)

Depositor

Stefan Stamm

Article

Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

Insert

SNORD27 (SNORD27 Human)

Use

Tags

no tag

Expression

Mammalian

Mutation

expression cassette for SNORD27 with AS box from …

Promoter

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only
RaCCR1_EYFP_pcDNA3.1

Plasmid

#159122

Purpose

This plasmid encodes RaCCR1 rhodopsin domain

Depositor

John Spudich

Article

Sineshchekov et al mBio. 2020 Apr 21;11(2). pii: mBio.00657

Insert

RaCCR1

Use

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1mLCAD_K42R_FLAG

Plasmid

#24486

Depositor

Eric Verdin

Article

Hirschey et al Nature. 2010 Mar 4. 464(7285):121-5.

Insert

Long chain acyl-CoA dehydrogenase (Acadl Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Lys42Arg

Promoter

Available since

April 30, 2010

Availability

Academic Institutions and Nonprofits only
PgACR1_EYFP_pcDNA3.1

Plasmid

#161028

Purpose

Expresses PgACR1 in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Proc Natl Acad Sci U S A. 2020 Sep 15;11

Insert

PgACR1

Use

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_hE2F7mg_2ntmut

Plasmid

#73074

Purpose

human E2F7 minigene (exons 11,12,13/introns cassette) with mutated snord27 binding site

Depositor

Stefan Stamm

Article

Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

Insert

E2F7 (E2F7 Human)

Use

Tags

Expression

Mammalian

Mutation

partial gene, exons 11,12,13 spaced by introns, 2…

Promoter

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only
pcDNA3.1_hE2F7mg_115mut

Plasmid

#73077

Purpose

human E2F7 minigene (exons 11,12,13/introns cassette) with inserted SNORD115 binding site

Depositor

Stefan Stamm

Article

Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

Insert

E2F7 (E2F7 Human)

Use

Tags

Expression

Mammalian

Mutation

partial gene, exons 11,12,13 spaced by introns, m…

Promoter

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1mLCAD_8KR_FLAG

Plasmid

#24485

Depositor

Eric Verdin

Article

Hirschey et al Nature. 2010 Mar 4. 464(7285):121-5.

Insert

Long chain acyl-CoA dehydrogenase (Acadl Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Lys42, 156, 189, 240, 254, 318, 322, 358Arg (8KR)

Promoter

Available since

April 30, 2010

Availability

Academic Institutions and Nonprofits only
hNav1.8_pcDNA3.1

Plasmid

#182847

Purpose

Expresses hNav1.8 in mammalian cells

Depositor

Paul DeCaen

Article

McCollum et al Cell Rep. 2022 Aug 23;40(8):111248. doi:

Insert

SCN10A (SCN10A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_hCAR.Variant3

Plasmid

#209133

Purpose

The ORF of human constitutive androstane receptor (CAR), variant 3 cDNA amplified from the RT human liver RNA was cloned at the NheI/HindIII sites of the pcDNA3.1(+), the mammalian expression vector.

Depositor

Reza Zolfaghari, Catharine Ross

Insert

constitutive androstane receptor (CAR), Variant 3 (NR1I3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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mAMCase_CatD_6xHis_pcDNA3.1

Plasmid

#200229

Purpose

Expresses mouse Acidic Mammalian Chitinase catalytic domain with C-terminal 6xHis tag in pcDNA 3.1 vector

Depositor

James S Fraser

Article

Diaz et al Elife. 2024 Jun 17;12:RP89918. doi: 10.7

Insert

Acidic Mammalian Chitinase (Chia1 Mouse)

Use

Tags

6xHis

Expression

Mammalian

Mutation

S354F

Promoter

CMV

Available since

Sept. 15, 2023

Availability

Academic Institutions and Nonprofits only

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HcCCR_mCherry_pcDNA3.1

Plasmid

#201687

Purpose

expresses HcCCR_mCherry fusion in mammalian cells

Depositor

John Spudich

Article

Govorunova et al mBio. 2022 Dec 20;13(6):e0303922. doi: 1

Insert

HcCCR

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

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CovKCR1_mCherry_pcDNA3.1

Plasmid

#201688

Purpose

expresses CovKCR1_mCherry fusion in mammalian cells

Depositor

John Spudich

Article

Govorunova et al mBio. 2022 Dec 20;13(6):e0303922. doi: 1

Insert

CovKCR1

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

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