We narrowed to 9,215 results for: pho;

1. pSaPIPLC His

   Plasmid

   #173810

   Purpose

   Expression and purification of mature, His-tagged Staphylococcus aureus (strain FPR3757) phosphaatidyl inositol specific phospholipase C (PI-PLC) from E. coli BL21 CodonPlus (DE3) RIL cells

   Depositor

   Mary F Roberts, Anne Gershenson

   Article

   Goldstein et al Biochemistry. 2012 Mar 27;51(12):2579-87

   Insert

   Staphylococcus aureus plc
   

   Use

   Tags

   6XHis

   Expression

   Bacterial

   Mutation

   none

   Promoter

   T7

   Available since

   Aug. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Lentiviral-His-tagged-PSPH

   Plasmid

   #134786

   Purpose

   Stable overexpression lentiviral vector of His tagged PSPH

   Depositor

   Seon-Young Kim

   Article

   Park et al Mol Cells. 2019 Aug 31;42(8):604-616. do

   Insert

   Phosphoserine phosphatase (PSPH Human)
   

   Use

   Lentiviral

   Tags

   His

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Dec. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGEX-GST-BcPC-PLC

   Plasmid

   #224255

   Purpose

   Express N-terminal HRV 3C protease cleavable GST fused to Bacillus cereus PC-PLC in E.coli

   Depositor

   Chiaki Murakami

   Article

   Murakami et al Anal Biochem. 2017 Jun 1;526:43-49. doi:

   Insert

   Bacillus cereus phospholipase C (plC, plC Bacillus cereus ATCC 14579)
   

   Use

   Tags

   GST and HRV-3C (LEVLFQGP) site

   Expression

   Bacterial

   Mutation

   The gene of Bacillus cereus ATCC 14579 was used f…

   Promoter

   lac promoter

   Available since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28a-Ec.coaBC (pCLK1210)

   Plasmid

   #50448

   Purpose

   Heterologous expression of E. coli CoA biosynthetic enzyme, PPCS_PPCDC - phosphopantothenoylcysteine synthetase_phosphopantothenoylcysteine decarboxylase

   Depositor

   Tadhg Begley, Erick Strauss

   Article

   Strauss et al J Biol Chem. 2001 Apr 27;276(17):13513-6

   Insert

   PPCS PPCDC
   

   Use

   Tags

   6XHIS tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. EcPhnD90.L1∆∆AD.L297RL301R.pRSET

   Plasmid

   #33376

   Purpose

   a single-wavelength phosphonate sensor constructed from E. coli PhnD and cpGFP

   Depositor

   Loren Looger

   Article

   Alicea et al J Mol Biol. 2011 Oct 12.

   Insert

   phnD based Phosphonate Biosensor (phnD E. coli)
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   cpGFP146 variant from GCaMP2 inserted in place of…

   Promoter

   T7

   Available since

   Jan. 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. mRFP-FKBP-INPP5E(D556A)

   Plasmid

   #183678

   Purpose

   Recruitable human type IV 5-phosphatase enzyme (catalytically inactive)

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   INPP5E (INPP5E Human)
   

   Use

   Tags

   mRFP-FKBP12(1-108)

   Expression

   Mammalian

   Mutation

   C641A, D556A

   Promoter

   CMV

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. cfSGFP2-anti-AlfaTag nanobody

   Plasmid

   #171818

   Purpose

   Bacterial expression vector for production of fluorescent anti-AlfaTag nanobody

   Depositor

   Ikuo Wada

   Article

   Matsumoto et al J Biol Chem. 2021 Jul;297(1):100851. doi

   Insert

   cfSGFP2-anti-AlfaTag nanobody
   

   Use

   Tags

   6xHis and SEP tag (6xD)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRK5-PLA2G15-6xHis WT

   Plasmid

   #213603

   Purpose

   Expresses PLA2G15-6xHis in mammalian cells

   Depositor

   Monther Abu-Remaileh

   Article

   Nyame et al Mol Cell. 2024 Apr 4;84(7):1354-1364.e9.

   Insert

   Phospholipase A2 Group XV (PLA2G15 Human)
   

   Use

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. tdTomato-anti-AlfaTag nanobody

   Plasmid

   #171819

   Purpose

   Bacterial expression vector for production of fluorescent anti-AlfaTag nanobody

   Depositor

   Ikuo Wada

   Article

   Matsumoto et al J Biol Chem. 2021 Jul;297(1):100851. doi

   Insert

   tdTomato-anti-AlfaTag nanobody
   

   Use

   Tags

   6xHis and SEP tag (6xD)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. SBFP2-anti-AlfaTag nanobody

    Plasmid

    #171826

    Purpose

    Bacterial expression vector for production of fluorescent anti-AlfaTag nanobody

    Depositor

    Ikuo Wada

    Article

    Matsumoto et al J Biol Chem. 2021 Jul;297(1):100851. doi

    Insert

    SBFP2-anti-AlfaTag nanobody
    

    Use

    Tags

    6xHis and SEP tag (6xD)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. p15aC-4D1D-5

    Plasmid

    #107866

    Purpose

    Bacterial expression of PIS, PI4K and PI4P5K

    Depositor

    Sanford Simon

    Article

    Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

    Inserts

    PIS
    phosphatidylinositol 4-phosphate 5-kinase
    phosphatidylinositol 4-kinase β
    

    Use

    Tags

    myc

    Expression

    Bacterial

    Mutation

    Promoter

    proD and proD (in operon)

    Available since

    April 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pNAS1b split mCherry 14-3-3β

    Plasmid

    #111880

    Purpose

    Mode #2 phosphosite expression (split mCherry, using 14-3-3β binding domain)

    Depositor

    Jesse Rinehart

    Article

    Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

    Type

    Empty backbone

    Use

    Tags

    Split mCherry system: 14-3-3β fused to C-terminal…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pNAS1b split mCherry 14-3-3σ

    Plasmid

    #111881

    Purpose

    Mode #2 phosphosite expression (split mCherry, using 14-3-3σ binding domain)

    Depositor

    Jesse Rinehart

    Article

    Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

    Type

    Empty backbone

    Use

    Tags

    Split mCherry system: 14-3-3σ fused to C-terminal…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. p15aC-3D1D-5

    Plasmid

    #121050

    Purpose

    Bacterial expression of PIS, PI4K and PI3P5K mutant E545K

    Depositor

    Sanford Simon

    Article

    Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

    Inserts

    PIS
    phosphatidylinositol 4-phosphate 5-kinase
    phosphatidylinositol 3-kinase catalytic subunit p110α of class IA E545K
    

    Use

    Tags

    myc and none

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRK5-PLA2G15-6xHis S198A

    Plasmid

    #213605

    Purpose

    Expresses PLA2G15-6xHis S198A in mammalian cells

    Depositor

    Monther Abu-Remaileh

    Article

    Nyame et al Mol Cell. 2024 Apr 4;84(7):1354-1364.e9.

    Insert

    Phospholipase A2 Group XV (PLA2G15 Human)
    

    Use

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Changed serine 198 to alanine

    Promoter

    CMV

    Available since

    March 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pNAS1b split mCherry NEDD4-2 WW2

    Plasmid

    #111883

    Purpose

    Mode #2 phosphosite expression (split mCherry, using NEDD4-2 WW2 binding domain)

    Depositor

    Jesse Rinehart

    Article

    Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

    Type

    Empty backbone

    Use

    Tags

    Split mCherry system: NEDD4-2 WW2 fused to C-term…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pNAS1b split mCherry NEDD4 WW2

    Plasmid

    #111882

    Purpose

    Mode #2 phosphosite expression (split mCherry, using NEDD4 WW2 binding domain)

    Depositor

    Jesse Rinehart

    Article

    Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

    Type

    Empty backbone

    Use

    Tags

    Split mCherry system: NEDD4 WW2 fused to C-termin…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. EGFP-IpgD

    Plasmid

    #183666

    Purpose

    N-terminally tagged Shigella flexneri IpgD for mammalian expression

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    IpgD
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pESC-LEU-IpgD

    Plasmid

    #183672

    Purpose

    Inducible Shigella flexneri IpgD for expression in yeast

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    IpgD
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. EGFP-SopB

    Plasmid

    #183657

    Purpose

    N-terminally tagged Salmonella Typhimurium SopB for mammalian expression

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    sopB (sopB Salmonella enterica serovar Typhimurium)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge