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Plasmid#100550PurposeExpresses ciCas9 in mammalian cells. Can be used to generate Flp-In and Flp-In T-REx stables.DepositorInsertciCas9
UseCRISPR; Frt, flp-inTagsFLAGExpressionMammalianMutationPromoterAvailable sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-pOTC-3H-GFP
Plasmid#205725PurposeExpression of GFP fused with pOTC-3H, a leader peptide from mitochondrial matrix enzyme ornithine transcarbamylase and a thermostable three helix-bundleDepositorInsertpOTC-3H-GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
hPRL3_1-169
Plasmid#209796PurposeBacterial expression of human PRL-3 without farnesylation motifDepositorInsertPRL-3 (PTP4A3 Human)
UseTags6xHisExpressionBacterialMutationaa 1-169 onlyPromoterT7Available sinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
786 pCSmAKT2Acherry
Plasmid#31167DepositorInsertAkt
UseExpression vectorTagsMyr and mCherryExpressionMutationmembrane tethered AktPromoterAvailable sinceSept. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
TtGNN-GFP-empty
Plasmid#106380Purposedox inducible expression of GFP with neo resistanceDepositorInsertGFP
UseRetroviralTagsExpressionMammalianMutationPromoterTRE-tightAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pME-6MTzactMap2k2b
Plasmid#67710PurposeMiddle entry cloneDepositorInsertactivated Map2k2b (map2k2b Zebrafish)
UseTagsExpressionMutationS221D, S225DPromoternoneAvailable sinceAug. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKJ458
Plasmid#228709PurposeMammalian expression of Antibody C143 HC R39K (heavy chain); to be used with pKJ460 (Plasmid 228710) to make the antibodyDepositorArticleInsertC143 HC R39K
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationPromoterAvailable sinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKJ460
Plasmid#228710PurposeMammalian expression of Antibody C143 LC N28R (light chain); to be used with pKJ458 (Plasmid 228709) to make the antibodyDepositorArticleInsertC143 LC N28R
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationPromoterAvailable sinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKJ423
Plasmid#228708PurposeMammalian expression of Anti-CD71 LC (light chain); to be used with pKJ455 (Plasmid 228707) to make the antibodyDepositorArticleInsertAnti-CD71 LC
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationPromoterAvailable sinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pXOOY
Plasmid#196449PurposeA dual vector for expression of membrane proteins in S. cerevisiae and X. laevisDepositorTypeEmpty backboneUseExpression in xenopus laevisTagsExpressionYeastMutationPromoteryeast CYC-GAL and T7 promoterAvailable sinceFeb. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-pOTC-GFP
Plasmid#205724PurposeExpression of GFP fused with pOTC, a leader peptide from mitochondrial matrix enzyme ornithine transcarbamylaseDepositorInsertpOTC-GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPEPT1
Plasmid#195001PurposeProduction of peptides in E. coli as GB1 fusionDepositorTypeEmpty backboneUseTagsHis8-tag and StrepTagII-GB1ExpressionBacterialMutationPromoterAvailable sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET15b/GVcl 1-1066 /*TBM
Plasmid#162781Purposeamplification of Gallus gallus Vcl mutated in Tankyrase Binding Domain IIDepositorArticleInsertGallus gallus vinculin (VCL Chicken)
UseTagsExpressionBacterialMutationchanged Gly 454 to ValPromoterAvailable sinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
EMX1*_sgRNA
Plasmid#100558PurposeExpresses EMX1* sgRNA. Target sequence: (G)GAGTCCGAGCAGAAGAAGAA. Same target sequence as #100555, except with an additional 5' G.DepositorInsertEMX1 sgRNA
UseTagsExpressionMutationPromoterU6Available sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pME-Fv2E-flt4-ns
Plasmid#67695Purposemiddle entry plasmid with dimerizable extracellular domain fused to Danio Flt4 cytoplasmic domainDepositorInsertFv2E-flt4
UseTagsExpressionMutationno stop codon; D1302E (see depositor's comme…PromoterAvailable sinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
LRG2.1
Plasmid#108098PurposeLentiviral expression plasmid of sgRNA with GFPDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 promoter for sgRNA expression and EFS promoter…Available sinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLVX-TetOne-Puro-GFP
Plasmid#171123PurposeDoxycycline inducible expression of GFPDepositorInsertEGFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lenti-luciferase-P2A-Neo
Plasmid#105621Purpose3rd generation lentiviral plasmid for expression of luciferase with Neo resistanceDepositorInsertLuciferase
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterEFSAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHAGE_puro
Plasmid#118692PurposeEmpty vector to express overexpression cDNADepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo FlucDM EGFP
Plasmid#90172PurposeEGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins. FLuc contains double mutationDepositorInsertFLuc EGFP
UseTagsExpressionMammalianMutationR188Q, R261Q in FLucPromoterAvailable sinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits only