We narrowed to 165,688 results for: addgene

1. pFA6a-natMX6-Purg1

   Plasmid

   #39350

   Depositor

   Jurg Bahler

   Article

   Watt et al PLoS One. 2008 Jan 16;3(1):e1428.

   Insert

   urg1 promoter (urg1, urg1 Fission Yeast)
   

   Use

   Yeast genomic targeting

   Tags

   natMX6 module - reverse orientation

   Available Since

   Nov. 8, 2012

   Availability

   Academic Institutions and Nonprofits only

2. pLKO.1 RagB shRNA #2

   Plasmid

   #26628

   Depositor

   David Sabatini

   Article

   Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

   Insert

   RagB shRNA (RRAGB Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   Oct. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

3. CRISPR_TRAC

   Plasmid

   #164993

   Purpose

   Expression of gRNA targeting TCRalpha constant locus

   Depositor

   Matthew Meyerson

   Article

   Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

   Insert

   gRNA against TRAC
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   March 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. attB53-Pac-attB53

   Plasmid

   #183610

   Purpose

   RMCE vector to shuttle puromycin resistance into attP50-flanked landing pad. Designed for use with pmROSA26-attP50-Neo-mKate2-3xNLS-attP50 vector (Addgene 183609).

   Depositor

   Sally Lowell

   Article

   Malaguti et al Development. 2022 May 26. pii: 275525. d

   Insert

   Pac
   

   Use

   Rmce

   Available Since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLentiCRISPRv2 sgSp1

   Plasmid

   #174907

   Purpose

   Second generation sgRNA against Sp1

   Depositor

   Jane Clifford

   Article

   Swift et al Cell Rep. 2021 Mar 16;34(11):108840. doi

   Insert

   Specificity Protein 1 (SP1 Human)
   

   Expression

   Mammalian

   Mutation

   Missense mutation to mutate PAM sequence

   Promoter

   EFS-NS

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRG710_lexO-HO_LexA-TF_HIS3MX

   Plasmid

   #154819

   Purpose

   Inducible expression of HO endonuclease in S. cerevisiae

   Depositor

   Lorraine Symington

   Article

   Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

   Inserts

   HO endonuclease (HO Budding Yeast)
   LexA-ER-B112
   

   Tags

   FLAG

   Expression

   Yeast

   Promoter

   ACT1 and lexO_4-CYC1_core

   Available Since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFA6a-link-ymVenus-URA3

   Plasmid

   #168058

   Purpose

   Replaces Addgene plasmid 118459. Yeast tagging vector to create a gene fusion with yeast optimized mVenus with CaUra3 selection; Contains the linker region at the 5’ end of the FP

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   ymVenus
   

   Tags

   ymVenus

   Expression

   Yeast

   Available Since

   June 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBabe hygro MTH1

   Plasmid

   #21296

   Depositor

   Bob Weinberg

   Article

   Rai et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

   Insert

   MTH1 (NUDT1 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   May 29, 2009

   Availability

   Academic Institutions and Nonprofits only

9. pSADdeltaG-GFP-ER(T2)CreER(T2)

   Plasmid

   #32649

   Depositor

   Edward Callaway

   Article

   Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

   Inserts

   GFP
   ER(T2)CreER(T2)
   

   Use

   Rabies virus

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 13, 2012

   Availability

   Academic Institutions and Nonprofits only

10. TET-pLKO.1 PURO shGFP

    Plasmid

    #83085

    Purpose

    Lentiviral shRNA vector for inducible knockdown of GFP (control hairpin)

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    shGFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

11. VV246: CoChR(C108S)-GCaMP6f-ER in fck

    Plasmid

    #58521

    Purpose

    Expresses CoChR(C108S) fused to GCaMP6f in mammalian cells

    Depositor

    Adam Cohen

    Article

    Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

    Insert

    CoChR(C108S)-GCaMP6f-ER
    

    Use

    Lentiviral

    Tags

    GCaMP6f

    Expression

    Mammalian

    Mutation

    changed Cysteine 108 to Serine

    Promoter

    a-CamKII

    Available Since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. TET-pLKO.1 PURO shLacZ

    Plasmid

    #98632

    Purpose

    Lentiviral shRNA vector for inducible knockdown of LacZ (control hairpin)

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    shLacZ
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pICH-chl-roGFP2-ORP1

    Plasmid

    #191699

    Purpose

    Ti plasmid pICH86988 harbouring chl-roGFP2-ORP1 for peroxide detection in the plastids of plant cells.

    Depositor

    Philip Mullineaux

    Article

    Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

    Insert

    chl-roGFP2-ORP1
    

    Use

    Binary agrobacterium ti plasmid

    Expression

    Plant

    Promoter

    CaMV 35S promoter

    Available Since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBigTB-FlpERT2

    Plasmid

    #149435

    Purpose

    tamoxifen-inducible Flp recombinase

    Depositor

    Primo Schaer

    Article

    Schwarz et al F1000Res. 2020 Sep 9;9:1112. doi: 10.126

    Insert

    Flp-ERT2 fusion protein
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    ERT2

    Expression

    Mammalian

    Promoter

    CAG promoter

    Available Since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. U6::sgBFP-U6::sgKrasG12D-pX333-red

    Plasmid

    #149448

    Purpose

    pX333 backbone expressing 2 sgRNA targeting BFP and KRAS G12D to edit mouse KRAS G12D allele.

    Depositor

    Jonathan Chernoff

    Article

    Budagyan et al Methods Mol Biol. 2021;2262:323-334. doi

    Insert

    sgRNA BFP and mouse KRAS G12D
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pENTR1A_no_ccDB_mUty

    Plasmid

    #158570

    Purpose

    V5-tagged sequence of mUty cloned into entry vector for recombination

    Depositor

    Christian Deschepper

    Article

    Deschepper et al Sci Rep. 2020 Sep 10;10(1):14900. doi: 1

    Insert

    Mus musculus ubiquitously transcribed tetratricopeptide repeat containing, Y-linked (Uty), transcript variant 2 (Uty Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKLV2-EF1a-NucGFP

    Plasmid

    #184696

    Purpose

    Lentiviral transfer plasmid for expression of NucGFP

    Depositor

    Brian Van Tine

    Article

    Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

    Insert

    NucGFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAGEN-SBP-DICER1

    Plasmid

    #50558

    Purpose

    purification of Dicer1 from mammalian cells

    Depositor

    Yukihide Tomari

    Article

    Betancur et al RNA. 2012 Jan;18(1):24-30. doi: 10.1261/

    Insert

    Dicer1 (Dicer1 Mouse)
    

    Tags

    SBP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pSLIK 3xFLAG-MTUS1 zeo

    Plasmid

    #192323

    Purpose

    Lentiviral expression vector for an inducible 3xFLAG-MTUS1

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

    Insert

    MTUS1 (MTUS1 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBabe-puro-miR-10b sponge

    Plasmid

    #25043

    Depositor

    Bob Weinberg

    Article

    Ma et al Nat Biotechnol. 2010 Apr . 28(4):341-7.

    Insert

    miR-10b sponge
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    June 11, 2010

    Availability

    Academic Institutions and Nonprofits only