We narrowed to 25,020 results for: lars

1. pcDNA3.1(+) ZKSCAN1 MCS-WT Split GFP + Sense IRES

   Plasmid

   #69909

   Purpose

   Expresses a GFP circular RNA containing the sense EMCV IRES in mammalian cells

   Depositor

   Jeremy Wilusz

   Article

   Kramer et al Genes Dev. 2015 Oct 8.

   Insert

   ZKSCAN1, GFP (ZKSCAN1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. YIP sHRPa-Aga1P

   Plasmid

   #73151

   Purpose

   Large fragment of split HRP fused to the N-terminus of the yeast mating protein Aga1P. When linearized, this vector incorporates into the yeast genome.

   Depositor

   Alice Ting

   Article

   Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

   Insert

   sHRPa-Aga1P
   

   Use

   Tags

   V5 tag

   Expression

   Yeast

   Mutation

   Promoter

   glyceraldehyde-3-phosphate dehydrogenase GPD prom…

   Available since

   June 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSyn-eLACCO1.1

   Plasmid

   #168788

   Purpose

   Neuronal expression of fluorescent sensor for extracellular lactate

   Depositor

   Marie-Eve Paquet

   Article

   Nasu et al Nat Commun. 2021 Dec 6;12(1):7058. doi:

   Insert

   eLACCO1.1
   

   Use

   AAV

   Tags

   ER export and GPI

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   April 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-GFAP-eLACCO1.1

   Plasmid

   #168790

   Purpose

   Astrocyte expression of fluorescent sensor for extracellular lactate

   Depositor

   Marie-Eve Paquet

   Article

   Nasu et al Nat Commun. 2021 Dec 6;12(1):7058. doi:

   Insert

   eLacco1.1
   

   Use

   AAV

   Tags

   ER export and GPI

   Expression

   Mammalian

   Mutation

   Promoter

   GFAP

   Available since

   April 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. FTL

   Plasmid

   #100750

   Purpose

   Part of FerriTag expression system. Untagged Ferritin light chain in mammalian expression vector.

   Depositor

   Stephen Royle

   Article

   Clarke et al Nat Commun. 2018 Jul 4;9(1):2604. doi: 1

   Insert

   FTL (FTL Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. LbCas12a-niCPE

   Plasmid

   #213747

   Purpose

   For circular RNA-mediated prime editor using LbCas12a-niCPE in HEK293T cells

   Depositor

   Caixia Gao

   Article

   Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

   Insert

   MCP-nLbCas12a-M-MLV RT∆RNase H
   

   Use

   CRISPR

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   D156R, R1138A in LbCas12a

   Promoter

   CMV

   Available since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCASB

   Plasmid

   #190175

   Purpose

   Plasmid for yeast expression of Cas9; contains a golden gate cloning site to additionally express a gRNA for a target sequence

   Depositor

   Jessica Tyler

   Article

   Aguilar et al Methods Protoc. 2022 Oct 4;5(5):79. doi:

   Insert

   sgRNA cloning site
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Added 5'-agagacc-3' upstream and 5'…

   Promoter

   Available since

   Oct. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CAG-eLACCO1.1

   Plasmid

   #168789

   Purpose

   Fluorescent sensor for extracellular lactate

   Depositor

   Marie-Eve Paquet

   Article

   Nasu et al Nat Commun. 2021 Dec 6;12(1):7058. doi:

   Insert

   eLACCO1.1
   

   Use

   AAV

   Tags

   ER export and GPI

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-Syn-VARNAM2

   Plasmid

   #195520

   Purpose

   Red fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter

   Depositor

   Vincent Pieribone

   Article

   Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

   Insert

   VARNAM2
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   VARNAM SI linker

   Promoter

   Synapsin

   Available since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1(+) Laccase2 MCS Exon Vector

    Plasmid

    #69893

    Purpose

    Expression plasmid for expressing circular RNAs of a desired sequence in mammalian cells

    Depositor

    Jeremy Wilusz

    Article

    Kramer et al Genes Dev. 2015 Oct 8.

    Insert

    Laccase2 (stw Fly)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET21b-Caspase-3

    Plasmid

    #90087

    Purpose

    Expresses wild type caspase 3 in bacterial cells

    Depositor

    Clay Clark

    Article

    Pop et al Biochemistry. 2001 Nov 27;40(47):14224-3

    Insert

    Caspase 3 (CASP3 Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Wild type

    Promoter

    Available since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUASTattB-dALFANb(5mut)-mGreenLantern

    Plasmid

    #219661

    Purpose

    Gal4-inducible expression of the destabilised anti-ALFAtag nanobody (including 5 destabilising mutations) fused to mGreenLantern

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    Destabilised ALFA nanobody (5 mutations) fused to mGreenLantern
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP VAMP4

    Plasmid

    #42313

    Depositor

    Thierry Galli

    Article

    Mallard et al J Cell Biol. 2002 Feb 18;156(4):653-64.

    Insert

    VAMP 4 (VAMP4 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDEST47-Arl13b12

    Plasmid

    #40872

    Depositor

    Tamara Caspary

    Article

    Larkins et al Mol Biol Cell. 2011 Dec;22(23):4694-703.

    Insert

    Arl13b (Arl13b Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    no stop codon

    Promoter

    CMV

    Available since

    Nov. 5, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRN3P-NLS-EGFP-Btgh-3NLS

    Plasmid

    #194469

    Purpose

    Encodes for bacterial O-GlcNAcase BtGH84 fused to enhanced GFP and NLSs for effective nuclear localization, in backbone for T3 In Vitro Transcription.

    Depositor

    Mathieu Boulard

    Article

    Formichetti et al Dev Cell 10 Jan 2024

    Insert

    BtGH84
    

    Use

    For in vitro transcription from t3 promoter after…

    Tags

    SV40 Nuclear Localization Signal, SV40 Nuclear Lo…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. VSVG-mCherry-RUSH

    Plasmid

    #203647

    Purpose

    RUSH cargo: Str-KDEL_IRES_SBP-mCherry-VSVG

    Depositor

    Juan Bonifacino

    Article

    Chen et al J Cell Biol. 2017 Dec 4;216(12):4141-415

    Insert

    VSVG
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. Peft-3::cas9-SV40_NLS::tbb-2 3'UTR

    Plasmid

    #46168

    Purpose

    Codon optimized Cas9_SV40 NLS with intron

    Depositor

    John Calarco

    Article

    Friedland et al Nat Methods. 2013 Jun 30. doi: 10.1038/n

    Insert

    codon optimized Cas9_SV40 NLS with intron
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    intron inserted at position 3113-3163, and SV40 N…

    Promoter

    eef-1A.1 (eft-3)

    Available since

    July 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pT2/CMV-Luc/PGK-SB100X

    Plasmid

    #192869

    Purpose

    Carries luciferase under CMV promoter flanked with IR/DR elements. Also carries SB100X under PGK promoter outside of the IR/DR elements.

    Depositor

    Maria Castro, David Largaespada

    Article

    Calinescu et al J Vis Exp. 2015 Feb 22;(96). doi: 10.379

    Inserts

    luc
    SB100X
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEM-HE-h_bPAC_cmyc

    Plasmid

    #28134

    Purpose

    optogenetic tool for light modulation of cellular cAMP

    Depositor

    Peter Hegemann

    Article

    Stierl et al J Biol Chem. 2011 Jan 14. 286(2):1181-8.

    Insert

    humanized photoactivated adenylyl cyclase
    

    Use

    In vitro transcription

    Tags

    Myc

    Expression

    Mutation

    Promoter

    Available since

    June 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKPI-MtLAP1

    Plasmid

    #193521

    Purpose

    Anthocyanin pigmentation based visual marker for arbuscular mycorrhizal fungal colonization of Medicago truncatula roots

    Depositor

    Jeremy Murray

    Article

    Kumar et al New Phytol. 2022 Sep 20. doi: 10.1111/np

    Inserts

    MtLAP1
    DsRed
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    ATUBI10 and Medtr8g059790(KPI)

    Available since

    June 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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