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pUPRT::DHFR-D

Plasmid

#58528

Purpose

replace TgUPRT with DHFR by homologous recombination

Depositor

David Sibley

Publication

Shen et al MBio. 2014 May 13;5(3). pii: e01114-14.

Insert

DHFR cassette

Use

Gateway Cloning

Mutation

pyrimethamine resistant

Promoter

DHFR

Available since

Aug. 6, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pDONR223-PRKAG3

Plasmid

#23549

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRKAG3 (PRKAG3 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
Caspase-Reporter

Plasmid

#243673

Purpose

Fluorescent Reporter for Caspase 3/7 activity. Contains DEVD sequence.

Depositor

Matthias Wirth

Publication

Selcen et al Cell Death Discov. 2025 Aug 6;11(1):368.

Insert

Split-GFP with DEVD sequence and T2A mCherry

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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Lenti-EFS-PEmax-P2A-Puro

Plasmid

#228890

Purpose

Lentiviral delivery and expression of PEmax protein with puromycin selection cassette.

Depositor

Francisco J Sánchez-Rivera

Publication

Gould et al Nat Biotechnol. 2024 Mar 12. doi: 10.103

Inserts

PEmax
Puromycin resistance

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/AUG-DAP5-3XFLAG

Plasmid

#127331

Purpose

Express 3xFLAG tagged DAP5 protein from AUG start codon

Depositor

Jeremy Wilusz

Publication

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

DAP5 (EIF4G2 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

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pGWB415

Plasmid

#74809

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with 3xHA (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Publication

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB417

Plasmid

#74811

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with 4xMyc (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Publication

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available since

July 14, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCXLE-dCas9VP192-T2A-EGFP-shP53

Plasmid

#69535

Purpose

Episomal plasmid encoding dCas9VP192 and p53 shRNA

Depositor

Timo Otonkoski

Publication

Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

dCas9-dCas9VP192-GFP-shp53

Use

CRISPR

Tags

EGFP

Promoter

CAG

Available since

Nov. 20, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pFPL-Gh

Plasmid

#61648

Purpose

Gateway vector for generating green fluorescent protein fusions in fungi

Depositor

Mark Farman

Publication

Gong et al Mol Plant Microbe Interact. 2014 Nov 12.

Insert

enhanced green fluorescent protein

Expression

Plant

Available since

Feb. 5, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pET26-MBP-GLIC

Plasmid

#20887

Depositor

Raimund Dutzler

Publication

Hilf et al Nature. 2009 Jan 1. 457(7225):115-8.

Insert

pentameric ligand gated ion channel from Gloeobacter violaceus PCC 7421

Tags

His, MBP, and pelB

Expression

Bacterial

Available since

Oct. 2, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCAG-rtTA

Plasmid

#163601

Purpose

Expresses rtTA in mammalian cells

Depositor

Takeshi Imai

Publication

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

rtTA

Expression

Mammalian

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pENTR221-NR1H3

Plasmid

#79514

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human NR1H3 CDS

Depositor

Peter Jon Nelson

Publication

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

LXR Alpha (NR1H3 Human)

Use

Gateway Cloning

Available since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAM1573

Plasmid

#40239

Depositor

Susan Golden

Publication

Andersson et al Methods Enzymol. 2000;305:527-42.

Insert

Cm Resistance, neutral site II integration platform for Synechococcus

Use

Cyanobacteria cloning vector

Promoter

n/a

Available since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBABE-Src-Rescue

Plasmid

#26983

Depositor

Joan Massague

Publication

Zhang et al Cancer Cell. 2009 Jul 7. 16(1):67-78.

Insert

c-Src (CSK Chicken)

Use

Retroviral

Expression

Mammalian

Available since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles
pDONR223-PIK3R4

Plasmid

#23488

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PIK3R4 (PIK3R4 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pDONR223-SGK

Plasmid

#23708

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

SGK (SGK1 Human)

Use

Gateway Cloning

Mutation

F430L

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLL39

Plasmid

#15458

Depositor

Chia Lee

Publication

Luong et al J Microbiol Methods. 2007 Jul . 70(1):18

Insert

Use

S. aureus-e. coli shuttle vector

Available since

Oct. 9, 2007

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG-FLEX.SF-iGluSnFR.A184S

Plasmid

#106186

Purpose

Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Publication

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.A184S

Use

AAV

Mutation

GltI: A184S

Promoter

CAG

Available since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pHLmMBP-3

Plasmid

#72345

Purpose

Mammalian expression of secreted N-terminally 8His-tagged mMBP-fused proteins with C-terminal 6His-tag/Strep-tag II/HA-tag

Depositor

Luca Jovine

Publication

Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii

Type

Empty backbone

Tags

6His-tag, 8His-tag, HA-tag, Strep-tag II, and mMB…

Expression

Mammalian

Promoter

CMV enhancer + chicken beta-actin promoter

Available since

Feb. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR223-PIK4CA

Plasmid

#23541

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PIK4CA (PI4KA Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only