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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 7,382 results for: PAC

Showing: 2801 - 2820 of 7382 results

  2. pOPO717

    Plasmid
    #195302
    Purpose
    pBAD322-MycaIDCas-lacZsp5, arapBAD promoter expressing type I-D Cascade and one spacer array of McCAST (Tn7575), the single spacer is lacZ spacer 5.
    Depositor
    Joseph Peters
    Article
    Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765
    Insert
    McCAST Cascade and lacZ spacer 5 in native array
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Arabinose inducible arapBAD
    Available since
    March 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    195302-plasmid-map-sequence-id-383138

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  3. pMOD_B2103

    Plasmid
    #91061
    Purpose
    Module B, Promoter: CmYLCV, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers , Terminator: 35S
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    CmYLCV
    Available since
    July 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    91061-plasmid-map-sequence-id-181853

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  5. pMOD_B2303

    Plasmid
    #91068
    Purpose
    Module B, Promoter: CmYLCV, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers , Terminator: 35S
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    CmYLCV
    Available since
    July 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    91068-plasmid-map-sequence-id-181874

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  6. pMOD_B2101

    Plasmid
    #91060
    Purpose
    Module B, Promoter: 35S, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers , Terminator: 35S
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    35S
    Available since
    July 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    91060-plasmid-map-sequence-id-181852

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  7. pMOD_B2112

    Plasmid
    #91064
    Purpose
    Module B, Promoter: PvUbi1, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers , Terminator: 35S
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    PvUbi1
    Available since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    91064-plasmid-map-sequence-id-181860

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  8. pMOD_B2203

    Plasmid
    #91066
    Purpose
    Module B, Promoter: CmYLCV, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers (only works with pMOD_C2200), Terminator: none - to be fused to gRNA array in MODULE C
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    CmYLCV
    Available since
    July 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    91066-plasmid-map-sequence-id-181864

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  9. pMOD_B2103b

    Plasmid
    #91062
    Purpose
    Module B, Promoter: CmYLCV, Gene: SapI ccdb cassette (doesn't include the promoter) for cloning multiple gRNA spacers with Csy4 spacers , Terminator: 35S
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    SapI ccdb cassette (no promoter) for cloning multiple gRNA spacers with Csy4 spacers
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    CmYLCV
    Available since
    July 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    91062-plasmid-map-sequence-id-181865

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  10. pMOD_B2312

    Plasmid
    #91070
    Purpose
    Module B, Promoter: PvUbi1, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers , Terminator: 35S
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    PvUbi1
    Available since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    91070-plasmid-map-sequence-id-181878

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  11. pMOD_B2301

    Plasmid
    #91067
    Purpose
    Module B, Promoter: 35S, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers , Terminator: 35S
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    35S
    Available since
    July 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    91067-plasmid-map-sequence-id-181873

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  12. pMOD_B2309

    Plasmid
    #91069
    Purpose
    Module B, Promoter: YAO, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers , Terminator: 35S
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    YAO
    Available since
    July 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    91069-plasmid-map-sequence-id-181875

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  13. pMOD_B2121

    Plasmid
    #91065
    Purpose
    Module B, Promoter: GmUbi, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers , Terminator: 35S
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    GmUbi
    Available since
    July 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    91065-plasmid-map-sequence-id-181862

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  14. pMOD_B2403

    Plasmid
    #91071
    Purpose
    Module B, Promoter: CmYLCV, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with ribozyme spacers , Terminator: 35S
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    SapI ccdb cassette for cloning multiple gRNA spacers with ribozyme spacers
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    CmYLCV
    Available since
    July 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    91071-plasmid-map-sequence-id-181880

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  15. VIPR2-Tango

    Plasmid
    #66521
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    VIPR2 (VIPR2 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  16. VIPR1-Tango

    Plasmid
    #66520
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    VIPR1 (VIPR1 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  17. pET-NlaCascade-NLS-6xHis

    Plasmid
    #180213
    Purpose
    Expressing Neisseria lactamica Type I-C Cascade subunits (Cas5-Cas8-Cas11-Cas7) with NLS and His tag on the C terminus of Cas7 for protein purification in E.Coli
    Depositor
    Yan Zhang
    Article
    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.
    Insert
    NlaCas5-Cas8-Cas7
    Use
    Tags
    6XHis and NLS
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    180213-plasmid-map-sequence-id-356902

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  18. pMOD_C2200

    Plasmid
    #91082
    Purpose
    Module C, Promoter: none – to be combined with gRNA array in module B, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers (only works with pMOD_B2203), Terminator: 35S
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    none (will be fused to gRNA array in MODULE B)
    Available since
    July 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    91082-plasmid-map-sequence-id-182046

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  19. VIPR1

    Plasmid
    #51865
    Purpose
    Expresses full-length Vasoactive intestinal polypeptide receptor 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    VIPR1 (VIPR1 Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51865-plasmid-map-sequence-id-79760

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  20. pNA0304

    Plasmid
    #165612
    Purpose
    Vector for expression of sgRNAs in yeast: SNR52p-NotI-sgRNA-SUP4t (NotI site in place of the spacer)
    Depositor
    Marcus Noyes
    Article
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    SNR52p-NotI-sgRNA-SUP4t (S. cerevisiae SNR52 promoter driving the sgRNA for SpCas9, with a NotI site in place of the spacer)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    NotI site replaced the CAN1 spacer in parent vect…
    Promoter
    SNR52
    Available since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    165612-plasmid-map-sequence-id-331071

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Showing: 2801 - 2820 of 7382 results