We narrowed to 4,867 results for: gne

1. pcDNA3.1-GSDMD_H270A_D275A

   Plasmid

   #224705

   Purpose

   Expression of mutated Gasdermin D_H270A_D275A (GSDMD_D275A) in transfected mammalian cell lines

   Depositor

   Christopher Overall

   Article

   Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

   Insert

   GSDMD_Q29A_H270A (GSDMD Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   H270A and D275A

   Promoter

   CMV

   Available since

   July 2, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. EFS-SpdCas9-Dnmt3A/3L-V1

   Plasmid

   #222498

   Purpose

   Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V1 (R887E) followed by P2A-mCherry

   Depositor

   Isaac Hilton

   Article

   Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021

   Insert

   S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V1 (R887E) engineered fusion
   

   Use

   Lentiviral

   Tags

   FLAG Tag

   Expression

   Mammalian

   Mutation

   D10A and H840A in S.pyogenes Cas9; R887E in Dnmt3A

   Promoter

   EFS

   Available since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. EFS-SpdCas9-Dnmt3A/3L-V2

   Plasmid

   #222499

   Purpose

   Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V2 (E814G) followed by P2A-mCherry

   Depositor

   Isaac Hilton

   Article

   Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021

   Insert

   S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V2 (E814G) engineered fusion
   

   Use

   Lentiviral

   Tags

   FLAG Tag

   Expression

   Mammalian

   Mutation

   D10A and H840A in S.pyogenes Cas9; E814G in Dnmt3A

   Promoter

   EFS

   Available since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. EFS-SpdCas9-Dnmt3A/3L-V3

   Plasmid

   #222500

   Purpose

   Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V3 (R887E and E814G) followed by P2A-mCherry

   Depositor

   Isaac Hilton

   Article

   Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021

   Insert

   S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V3 (R887E and E814G) engineered fusion
   

   Use

   Lentiviral

   Tags

   FLAG Tag

   Expression

   Mammalian

   Mutation

   D10A and H840A in S.pyogenes Cas9; R887E and E814…

   Promoter

   EFS

   Available since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. TRE3GV-SpdCas9-Dnmt3A/3L-V1

   Plasmid

   #222503

   Purpose

   Expresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V1 (R887E) and hPGK promoter driven mCherry

   Depositor

   Isaac Hilton

   Article

   Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021

   Inserts

   S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V1 (R887E) engineered fusion
   mCherry
   

   Use

   Lentiviral

   Tags

   FLAG Tag

   Expression

   Mammalian

   Mutation

   D10A and H840A in S.pyogenes Cas9; R887E in Dnmt3A

   Promoter

   TRE3GV and hPGK

   Available since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. TRE3GV-SpdCas9-Dnmt3A/3L-V2

   Plasmid

   #222505

   Purpose

   Expresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V2 (E814G) and hPGK promoter driven mCherry

   Depositor

   Isaac Hilton

   Article

   Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021

   Inserts

   S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V2 (E814G) engineered fusion
   mCherry
   

   Use

   Lentiviral

   Tags

   FLAG Tag

   Expression

   Mammalian

   Mutation

   D10A and H840A in S.pyogenes Cas9; E814G in Dnmt3A

   Promoter

   TRE3GV and hPGK

   Available since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. TRE3GV-SpdCas9-Dnmt3A/3L-V3

   Plasmid

   #222506

   Purpose

   Expresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V3 (R887E and E814G) and hPGK promoter driven mCherry

   Depositor

   Isaac Hilton

   Article

   Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021

   Inserts

   S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V3 (R887E and E814G) engineered fusion
   mCherry
   

   Use

   Lentiviral

   Tags

   FLAG Tag

   Expression

   Mammalian

   Mutation

   D10A and H840A in S.pyogenes Cas9; R887E and E814…

   Promoter

   TRE3GV and hPGK

   Available since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. TRE3GV-SpdCas9-Dnmt3A/3L-catΔ

   Plasmid

   #222508

   Purpose

   Expresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant catΔ (C706A and R832E) and hPGK promoter driven mCherry

   Depositor

   Isaac Hilton

   Article

   Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021

   Inserts

   S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant catΔ (C706A and R832E) engineered fusion
   mCherry
   

   Use

   Lentiviral

   Tags

   FLAG Tag

   Expression

   Mammalian

   Mutation

   D10A and H840A in S.pyogenes Cas9; C706A and R832…

   Promoter

   TRE3GV and hPGK

   Available since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCR8 JARID2

   Plasmid

   #114443

   Purpose

   Entry vector for gateway cloning containing human JARID2 coding sequence

   Depositor

   Adrian Bracken

   Article

   Conway et al Mol Cell. 2018 May 3;70(3):408-421.e8. d

   Insert

   JARID2 (JARID2 Human)
   

   Use

   Gateway entry cloning

   Tags

   Expression

   Mutation

   Promoter

   None

   Available since

   Sept. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCR8 PALI1

    Plasmid

    #114442

    Purpose

    Entry vector for gateway cloning containing human PALI1 coding sequence

    Depositor

    Adrian Bracken

    Article

    Conway et al Mol Cell. 2018 May 3;70(3):408-421.e8. d

    Insert

    PALI1 (LCOR Human)
    

    Use

    Gateway entry cloning

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    Sept. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV-CMV-DIO-TRPV4-p2A-ferritin-sNRPpA

    Plasmid

    #74306

    Purpose

    Bicistronic Cre-dependent expression of TRPV4 & ferritin in mammalian cells

    Depositor

    Ali Guler

    Article

    Wheeler et al Nat Neurosci. 2016 Mar 7. doi: 10.1038/n

    Inserts

    TRPV4 (Trpv4 Rat)
    ferritin (FTL and HTL1) (FTL, FTH1 Human)
    

    Use

    AAV and Cre/Lox

    Tags

    FLAG and p2A

    Expression

    Mammalian

    Mutation

    Promoter

    CMV (after cre recombination)

    Available since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCR8 LCOR

    Plasmid

    #114441

    Purpose

    Entry vector for gateway cloning containing human LCOR coding sequence

    Depositor

    Adrian Bracken

    Article

    Conway et al Mol Cell. 2018 May 3;70(3):408-421.e8. d

    Insert

    LCOR (LCOR Human)
    

    Use

    Gateway entry cloning

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    Sept. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pVL-FLAG/His PALI1

    Plasmid

    #114449

    Purpose

    Insect baculovirus expression vector containing N terminal FLAG/His tagged human PALI1 coding sequence

    Depositor

    Adrian Bracken

    Article

    Conway et al Mol Cell. 2018 May 3;70(3):408-421.e8. d

    Insert

    PALI1 (LCOR Human)
    

    Use

    Tags

    FLAG-His

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1-GSDMD_1-270

    Plasmid

    #218229

    Purpose

    Gasdermin1-270 is a pore-forming GSDMD formed by SARS-CoV-2 3CLpro cleavage. Transfection in HEK293 cells showed GSDMD 1-270 pores secrete 3CLpro, nucleocapsid, and in excess, kill cells by pyroptosis

    Depositor

    Christopher Overall

    Article

    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

    Insert

    GSDMD (GSDMD Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    construct only codes for amino acids 1-270

    Promoter

    Available since

    July 1, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pcDNA3.1-GSDMD_1-275

    Plasmid

    #218230

    Purpose

    Gasdermin-D_1-275 is a pore-forming GSDMD formed by cleavage by host caspases. Transfection in HEK293 cells showed GSDMD 1-275 pores secrete 3CLpro, nucleocapsid; in excess, kills cells by pyroptosis

    Depositor

    Christopher Overall

    Article

    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

    Insert

    GSDMD (GSDMD Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    construct only codes for amino acids 1-275

    Promoter

    Available since

    July 1, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pcDNA3.1-GSDMD_29-484

    Plasmid

    #218231

    Purpose

    Gasdermin-D_29-484 is a non-functional GSDMD formed by SARS-CoV-2 3CLpro cleavage in the N-terminal domain. Transfection in HEK293 cells showed no pore-forming activity or induction of pyroptosis

    Depositor

    Christopher Overall

    Article

    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

    Insert

    GSDMD (GSDMD Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    construct only codes for amino acids 29-484

    Promoter

    Available since

    July 1, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pcDNA3.1-GSDMD_29-270

    Plasmid

    #218232

    Purpose

    Gasdermin-D_29-270 is a non-functional GSDMD formed by two SARS-CoV-2 3CLpro cleavages. Transfection in HEK293 cells showed no pore-forming activity or induction of pyroptosis

    Depositor

    Christopher Overall

    Article

    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

    Insert

    GSDMD (GSDMD Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    construct only codes for amino acids 29-270

    Promoter

    Available since

    July 1, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pGEX6P1 GST-hUSP27X K181E

    Plasmid

    #225714

    Purpose

    Bacterial expression of USP27X (K181E) with GST tag

    Depositor

    Francisco Bustos

    Article

    Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

    Insert

    USP27X K181E (USP27X Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    K181E

    Promoter

    Available since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAGGS puro 3XFLAG-hATXN7L3

    Plasmid

    #225725

    Purpose

    Transfection of ATXN7L3 with 3XFLAG tag in mammalian cells

    Depositor

    Francisco Bustos

    Article

    Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

    Insert

    ATXN7L3 (ATXN7L3 Human)
    

    Use

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAGGS puro HA-hUSP27X S404N

    Plasmid

    #225724

    Purpose

    Transfection of USP27X (S404N) with HA tag in mammalian cells

    Depositor

    Francisco Bustos

    Article

    Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

    Insert

    USP27X S404N (USP27X Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    S404N

    Promoter

    Available since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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