We narrowed to 5,266 results for: 290

1. pCGT-HCF-1-C600

   Plasmid

   #99364

   Purpose

   C-term of HCFC1 protein

   Depositor

   Winship Herr

   Article

   Wilson et al Mol Cell Biol. 2000 Sep;20(18):6721-30.

   Insert

   HCF-1 C-term 1436-2035 (HCFC1 Human)
   

   Tags

   2x tandem T7 tag and C-myc

   Expression

   Mammalian

   Mutation

   Deletion of HCFC1 cDNA

   Promoter

   CMV

   Available Since

   Sept. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPN186

   Plasmid

   #91573

   Purpose

   Express sgRNA targeting human AKT3

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

   Insert

   AKT3 (AKT3 Human)
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   Oct. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. Lenti-sgNf2#1/Cre

   Plasmid

   #173643

   Purpose

   Expresses a Nf2-targeting gRNA and Cre-recombinase

   Depositor

   Charles Swanton

   Article

   Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

   Insert

   gRNA targeting Nf2 (Nf2 Mouse)
   

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Available Since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLKO_HSV.Ollas.S_mCherry-NLS

   Plasmid

   #178276

   Purpose

   Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

   Depositor

   Brian Brown

   Article

   Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

   Insert

   nPC Tagged mCherry-NLS
   

   Use

   Lentiviral

   Tags

   HSV.Ollas.S and Nuclear Localization Signal

   Promoter

   eF1a

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO_HA.HSV.Ollas_mCherry-NLS

   Plasmid

   #178259

   Purpose

   Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

   Depositor

   Brian Brown

   Article

   Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

   Insert

   nPC Tagged mCherry-NLS
   

   Use

   Lentiviral

   Tags

   HA.HSV.Ollas and Nuclear Localization Signal

   Promoter

   eF1a

   Available Since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLKO_HA.HSV.NWS_mCherry-NLS

   Plasmid

   #178258

   Purpose

   Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

   Depositor

   Brian Brown

   Article

   Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

   Insert

   nPC Tagged mCherry-NLS
   

   Use

   Lentiviral

   Tags

   HA.HSV.NWS and Nuclear Localization Signal

   Promoter

   eF1a

   Available Since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. Lenti-sgSmad4#1/Cre

   Plasmid

   #173617

   Purpose

   Expresses a Smad4-targeting gRNA and Cre-recombinase

   Depositor

   Charles Swanton

   Article

   Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

   Insert

   gRNA targeting Smad4 (Smad4 Mouse)
   

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Available Since

   June 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPN008

   Plasmid

   #91576

   Purpose

   Express sgRNA targeting human BCL11A

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

   Insert

   BCL11A (BCL11A Human)
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   Oct. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. plko-tet-on-puromycin-shTRX1-259

   Plasmid

   #110920

   Purpose

   To knockdown Thioredoxin-1 following doxycycline addition

   Depositor

   Priyamvada Rai

   Article

   Samaranayake et al Nat Commun. 2017 Oct 31;8(1):1204. doi:

   Insert

   TRX1 (TXN Human)
   

   Use

   Lentiviral

   Promoter

   unknown

   Available Since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPN187

    Plasmid

    #91574

    Purpose

    Express sgRNA targeting human AKT3

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    AKT3 (AKT3 Human)
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcdna3 Flag Jip3b(T266A, T276A, T287A)

    Plasmid

    #58924

    Purpose

    interacts with MAPK signaling module

    Depositor

    Roger Davis

    Article

    Kelkar et al Mol Cell Biol. 2000 Feb;20(3):1030-43.

    Insert

    mitogen-activated protein kinase 8 interacting protein 3 (Mapk8ip3 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    changed T266A, T276A, T287A

    Available Since

    Sept. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pLenti CMV Hygro DHFR.cHSF1

    Plasmid

    #134738

    Purpose

    Lentiviral expression vector for constitutively active HSF1 fused to DHFR destabilized domain

    Depositor

    Matthew D. Shoulders

    Article

    Phillips et al Elife. 2017 Sep 26;6. doi: 10.7554/eLife

    Insert

    DHFR.cHSF1 (HSF1 Human)
    

    Use

    Lentiviral

    Tags

    DHFR

    Expression

    Mammalian

    Mutation

    Deletion of amino acids 186-202

    Promoter

    CMV

    Available Since

    Oct. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHR-mCherry-p2A-LIC1 G domain

    Plasmid

    #74602

    Purpose

    expresses LIC1 in mammalian cells; aa 1-389, untagged but cells indicate expression with diffuse mCherry

    Depositor

    Ron Vale

    Article

    Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

    Insert

    full length human dynein light intermediate chain 1 amino acids 1-389 (DYNC1LI1 Human)
    

    Use

    Lentiviral

    Tags

    HA tag

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    May 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEBTetBl-CLIP-Cep70

    Plasmid

    #136870

    Purpose

    Mammalian expression of the centrosomal protein Cep70 N-terminally fused to CLIP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    CLIP-Cep70 (CEP70 Human, Synthetic)
    

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Promoter

    CMV-TetO2

    Available Since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEBTetDBl-CLIP-Cep70

    Plasmid

    #136871

    Purpose

    Mammalian expression of the centrosomal protein Cep70 N-terminally fused to CLIP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    CLIP-Cep70 (CEP70 Human, Synthetic)
    

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Promoter

    CMV-TetO2

    Available Since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX chimerin-SH2

    Plasmid

    #46415

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    chimerin (CHN1 Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 44-147

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. GST-CHMP4A (205-222) E209A (pGEX2T(TEV))

    Plasmid

    #80640

    Purpose

    Residues 205-222 of CHMP4A, Mutation modestly disrupts binding to ALIX; Bacterial Expression Vector; TEV-cleavable GST-tag; Sundquist Lab Internal ID: WISP06-206

    Depositor

    Wesley Sundquist

    Article

    McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105

    Insert

    CHMP4A (CHMP4A Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Changed Glutamate 209 to alanine

    Available Since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX6P1-human LIC1 G domain

    Plasmid

    #74598

    Purpose

    used for expression in E. coli and purification. N-terminal GST tag and C-terminal Strep tag, aa 1-389

    Depositor

    Ron Vale

    Article

    Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

    Insert

    human dynein light intermediate chain 1 amino acids 1-389 (DYNC1LI1 Human)
    

    Tags

    N-terminal GST and Strep II

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLJM5-I560S-mDPYD

    Plasmid

    #61615

    Purpose

    The mouse Dpyd was used to rescue the human DPYD hairpin affect on EMT

    Depositor

    David Sabatini

    Article

    Shaul et al Cell. 2014 Aug 28;158(5):1094-109. doi:

    Insert

    Dihydropyrimidine dehydrogenase (Dpyd Mouse)
    

    Expression

    Mammalian

    Mutation

    Changed Isoleucine at position 560 to Serine

    Promoter

    CMV

    Available Since

    Jan. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHCFnc/V5

    Plasmid

    #247003

    Purpose

    Expresses non cleavable HCF-1 with carboxy terminal V5 tag under control of the CMV promoter

    Depositor

    Thomas Kristie

    Article

    Vogel et al Proc Natl Acad Sci U S A. 2006 May 2;103

    Insert

    HCF-1 (HCFC1 Human)
    

    Tags

    V5 tag

    Expression

    Mammalian

    Mutation

    Glu to Ala change at amino acid positions 1019,10…

    Available Since

    Oct. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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