We narrowed to 7,099 results for: Ank

1. pAR18_StrepI_mFASm_H8_pET22b

   Plasmid

   #122847

   Purpose

   Expresses murine type I fatty acid synthase (FASN) in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.

   Depositor

   Martin Grininger

   Article

   Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi

   Insert

   mouse FASN/ mouse fatty acid synthase (Fasn Mouse)
   

   Use

   Tags

   His8 tag and Twin-Strep tag

   Expression

   Bacterial

   Mutation

   wildtype

   Promoter

   T7 promoter

   Available since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5-Flag-KIAA0947

   Plasmid

   #49248

   Purpose

   Mammalian expression of flag-tagged human KIAA0947

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

   Insert

   KIAA0947 (ICE1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pENTR221-EF1.Pro

   Plasmid

   #79488

   Purpose

   MultiSite Gateway entry clones, first fragment, (attL1, attR5) Human EF1 promoter

   Depositor

   Peter Jon Nelson

   Article

   Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

   Insert

   EF1 Promoter (EEF1A1 Human, Synthetic)
   

   Use

   Multisite gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   EF1 Promoter

   Available since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFC902

   Plasmid

   #106904

   Purpose

   The vector has a U3 promoter and terminator controlled gene encoding a transcript containing the sgRNA flanked by a tRNA-Gly repeat; and a gene encoding cas9 controlled by Ptef1 and Ttef1

   Depositor

   Uffe Mortensen

   Article

   Nodvig et al Fungal Genet Biol. 2018 Jan 8. pii: S108

   Inserts

   cas9
   pyrG
   U3 promoter and terminator controlled gene encoding a transcript containing the sgRNA flanked by a tRNA gly repeat
   

   Use

   CRISPR; Fungal expression

   Tags

   SV40 NLS

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. MBP-FUS_FL_6E

   Plasmid

   #98655

   Purpose

   Expresses MBP-tagged full length phosphomimetic FUS

   Depositor

   Nicolas Fawzi

   Article

   Monahan et al EMBO J. 2017 Aug 8. pii: e201696394. doi

   Insert

   FUS (FUS Human)
   

   Use

   Tags

   6xHis-MBP

   Expression

   Bacterial

   Mutation

   6 S/T to E

   Promoter

   Available since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEF1a-hMLH1dn (og codon)

   Plasmid

   #174823

   Purpose

   Mammalian expression of human MLH1dn (original codon usage)

   Depositor

   David Liu

   Article

   Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

   Insert

   human MLH1dn (original codon usage) (MLH1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   deletion of residues 754-756

   Promoter

   EF1a

   Available since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-flex-GFP-shRNA-GluN1

   Plasmid

   #182502

   Purpose

   Expresses shRNA against mouse NMDA receptor NR1 subunit. Flexed cassette driven by hdc (pan neuronal) gene promoter

   Depositor

   William Wisden

   Article

   Miracca et al J Neurosci. 2022 May 31. pii: JNEUROSCI.

   Insert

   pan neuronal promoter (hdc basal promoter) driving flexed-GFP-mir30-shRNA NR1 cassette
   

   Use

   AAV, Cre/Lox, and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   fragment of histidine decarboxylase gene promoter…

   Available since

   March 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFB-EPCP5

   Plasmid

   #228879

   Purpose

   Expression of Z-tagged LRRK2 KW using Baculoviruses

   Depositor

   Sebastian Mathea

   Article

   Raig et al bioRxiv [Preprint]. 2025 Feb 13:2024.09.

   Insert

   Leucine-rich repeat kinase 2 (LRRK2 Human)
   

   Use

   Tags

   His-Z-TEV

   Expression

   Insect

   Mutation

   Amino acids deleted 1-1846

   Promoter

   Available since

   Jan. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-TRE-DIO-Casp3-TEV

   Plasmid

   #183766

   Purpose

   This plasmid is for use with neuronal cell-type selective activity tagging. The Caspase3 expression requires both neural activity and Cre recombinase.

   Depositor

   William Wisden

   Article

   Yu et al Science. 2022 Jul;377(6601):63-72. doi:

   Insert

   AAV transgene - tetO promoter-flex/DIO-Caspase3-TEV
   

   Use

   AAV, Cre/Lox, and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tetO promoter

   Available since

   May 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. CPSF1

    Plasmid

    #155475

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    CPSF1 (CPSF1 Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV_AR-RE_MLPmin_BC1011-luc2

    Plasmid

    #227106

    Purpose

    Androgen receptor response element for luciferase and barcode assays; barcode BC1011

    Depositor

    Michael Wehr

    Article

    Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

    Insert

    8x clustered AR response element linked to MLP minimal promoter driving barcode 1011 and a luciferase reporter gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV_AR-RE_MLPmin_BC1014-luc2

    Plasmid

    #227108

    Purpose

    Androgen receptor response element for luciferase and barcode assays; barcode BC1014

    Depositor

    Michael Wehr

    Article

    Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

    Insert

    8x clustered AR response element linked to MLP minimal promoter driving barcode 1014 and a luciferase reporter gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-Zeo_BACE1

    Plasmid

    #227105

    Purpose

    BACE1 enzyme overexpression for Nrg1 activation in luciferase and barcode assays

    Depositor

    Michael Wehr

    Article

    Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

    Insert

    BACE1 (BACE1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYTSK01K_0G7

    Plasmid

    #177296

    Purpose

    genomic integration of genes (inserted in I-SceI-site) via transposon Tn7 with GmR

    Depositor

    Karl-Erich Jaeger

    Article

    Tiso et al Front Bioeng Biotechnol. 2020 Aug 20;8:9

    Insert

    PaacC1-aacC1
    

    Use

    Synthetic Biology; Yeast expression, tn7 genomic …

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. BTG1

    Plasmid

    #155425

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    BTG1 (BTG1 Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBa.GFP-KIF1Bbeta tail 387-1770

    Plasmid

    #134620

    Purpose

    Mammalian expression of mouse KIF1Bbeta 387-1170

    Depositor

    Marvin Bentley

    Article

    Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

    Insert

    KIF1Bbeta (Kif1b Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    WT with deletion of the 386 N-terminal residues.

    Promoter

    Chicken Beta actin

    Available since

    April 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. CSTF1

    Plasmid

    #155485

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    CSTF1 (CSTF1 Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV_ER-RE_MLPmin_BC1063-luc2

    Plasmid

    #227109

    Purpose

    Estrogen receptor response element for luciferase and barcode assays; barcode BC1063

    Depositor

    Michael Wehr

    Article

    Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

    Insert

    12x clustered ER response element linked to MLP minimal promoter driving barcode 1063 and a luciferase reporter gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDUET-1-alpha-synuclein-mCerulean3-His6

    Plasmid

    #110061

    Purpose

    For bacterial expression of human alpha-synuclein, carboxyl terminally tagged with mCerulean3 and poly-histidines

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/187

    Insert

    alpha-synuclein (SNCA Human)
    

    Use

    Tags

    6 x His and mCerulean3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET21a-C2m2-mKate

    Plasmid

    #208765

    Purpose

    To express C2m2-mKate in bacteria

    Depositor

    Urte Neniskyte

    Article

    Dirvelyte et al Cell Mol Biol Lett. 2023 Jul 27;28(1):59

    Insert

    C2m2-mKate (Mfge8 Mouse)
    

    Use

    Tags

    mKate

    Expression

    Bacterial

    Mutation

    Mutated 24 and 45 lysine to asparagine in C2 doma…

    Promoter

    T7 promoter

    Available since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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