We narrowed to 21,628 results for: ESC

1. pRSETa-mMaple3-V157I

   Plasmid

   #98577

   Purpose

   photoconvertible fluorescent protein mMaple3-V157I in pRSetA plasmid, photoconvertible by primed conversion mechanism, bacterial overexpression, sequence is codon optimised for E. coli

   Depositor

   Ulrike Endesfelder

   Article

   Turkowyd et al Angew Chem Int Ed Engl. 2017 Jun 2. doi:

   Insert

   mMaple3-V157I
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-mNeptune2.5

   Plasmid

   #51310

   Purpose

   Expresses far-red fluorescent protein mNeptune2.5 in mammalian cells

   Depositor

   Michael Lin

   Article

   Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

   Insert

   mNeptune2.5
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHR-CMV-TetO2_3C-Twin-Strep_IRES-mRuby2

   Plasmid

   #113885

   Purpose

   Mammalian (inducible) protein expression, bicistronic expression of cytosolic mRuby2

   Depositor

   A. Radu Aricescu

   Article

   Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3C-Twin-Strep

   Expression

   Mutation

   Promoter

   CMV-MIE-TetO2

   Available since

   Aug. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKD068

   Plasmid

   #136471

   Purpose

   SSB-GFP IDL fusion expression plasmid

   Depositor

   James Keck

   Article

   Dubiel et al Nucleic Acids Res. 2020 Jun 19;48(11):60

   Insert

   SSB-GFP (ssb E. coli)
   

   Use

   Tags

   superfolder GFP

   Expression

   Bacterial

   Mutation

   superfolder GFP inserted between Phe148 and Ser149

   Promoter

   T7 promoter

   Available since

   April 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC-GFP-HS

   Plasmid

   #133305

   Purpose

   "Burdensome" GFP expressing plasmid carrying hok/sok toxin-antitoxin for plasmid stabilisation

   Depositor

   Chris Barnes

   Article

   Fedorec et al iScience. 2019 Apr 26;14:323-334. doi: 1

   Insert

   hok/sok toxin-antitoxin cassette
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. WWP-Luc (p21/WAF1 promoter)

   Plasmid

   #16451

   Depositor

   Bert Vogelstein

   Article

   el-Deiry et al Cell. 1993 Nov 19. 75(4):817-25.

   Insert

   WAF1 promoter (CDKN1A Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 11, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTol1-UAS:GtACR2-tdTomato

   Plasmid

   #124236

   Purpose

   Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin GtACR2 fused to the red fluorescent protein tdTomato

   Depositor

   Isaac Bianco

   Article

   Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

   Insert

   GtACR2-tdTomato
   

   Use

   Zebrafish expression

   Tags

   tdTomato

   Expression

   Mutation

   Promoter

   14xUAS-E1b

   Available since

   Jan. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHR-CMV-TetO2_3C-Avi-His6__IRES-EmGFP

   Plasmid

   #113888

   Purpose

   Mammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of cytosolic EmGFP

   Depositor

   A. Radu Aricescu

   Article

   Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3C-Avi-His6

   Expression

   Mutation

   Promoter

   CMV-MIE-TetO2

   Available since

   Aug. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPBbsr-AcGFP1-ORP9-PH

   Plasmid

   #214270

   Purpose

   Mammalian expression of green fluorescent PI4P reporter AcGFP1-ORP9-PH

   Depositor

   Shinya Tsukiji

   Article

   Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

   Insert

   AcGFP1-ORP9(1-111) (Osbpl9 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGP-AAV-GFAP-iGABASnFR2-WPRE

    Plasmid

    #218871

    Purpose

    AAV-mediated expression of improved GABA sensor (positive change in fluorescence)

    Depositor

    GENIE Project

    Article

    Kolb et al bioRxiv 2025.03.25.644953

    Has Service

    AAV1 and AAV5

    Insert

    iGABASnFR2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    S99A F102Y F104Y L178S

    Promoter

    GFAP

    Available since

    May 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTol1-UAS:CoChR-tdTomato

    Plasmid

    #124233

    Purpose

    Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin CoChR fused to the red fluorescent protein tdTomato

    Depositor

    Isaac Bianco

    Article

    Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

    Insert

    CoChR-tdTomato
    

    Use

    Zebrafish expression

    Tags

    tdTomato

    Expression

    Mutation

    Promoter

    14xUAS-E1b

    Available since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3/er-(n2)oxStayGold(c4)

    Plasmid

    #185822

    Purpose

    A soluble photostable fluorescent marker of the ER lumen. The StayGold gene has no codon optimization for mammalian expression.

    Depositor

    Atsushi Miyawaki

    Article

    Hirano et al Nat Biotechnol. 2022 Apr 25. pii: 10.103

    Insert

    er-(n2)oxStayGold(c4)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGP-AAV-CAG-flex-iGABASnFR2(no bind)-WPRE

    Plasmid

    #218882

    Purpose

    AAV-mediated expression of improved GABA sensor control (no change in fluorescence)

    Depositor

    GENIE Project

    Article

    Kolb et al bioRxiv 2025.03.25.644953

    Insert

    iGABASnFR2(no bind)
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    S99A F102G R168P

    Promoter

    CAG

    Available since

    June 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMRE-Tn7-152

    Plasmid

    #118566

    Purpose

    miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    sGFP2
    

    Use

    Minitn7 delivery vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMRE-Tn7-142

    Plasmid

    #118558

    Purpose

    miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    sGFP2
    

    Use

    Minitn7 delivery vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. NPY-pHTomato

    Plasmid

    #83501

    Purpose

    Expression of a fluorescent granule marker, neuropeptide-Y, for localization and exocytosis studies.

    Depositor

    Sebastian Barg

    Article

    Gandasi et al PLoS One. 2015 Jun 19;10(6):e0127801. do

    Insert

    NPY (NPY Human)
    

    Use

    Tags

    pHTomato

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGP-AAV-syn-flex-iGABASnFR2n-WPRE

    Plasmid

    #218878

    Purpose

    AAV-mediated expression of improved GABA sensor (negative change in fluorescence)

    Depositor

    GENIE Project

    Article

    Kolb et al bioRxiv 2025.03.25.644953

    Insert

    iGABASnFR2n
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    S99A F104H R168P

    Promoter

    Synapsin

    Available since

    June 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMRE-Tn5-145

    Plasmid

    #118529

    Purpose

    miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mScarlet-I
    

    Use

    Minitn5 delivery vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. SuperClomeleon

    Plasmid

    #203724

    Purpose

    An improved engineered fluorescent ratiometric optogenetic sensor to detect inhibitory synaptic activity via changes in intracellular chloride

    Depositor

    George Augustine

    Article

    Grimley et al J Neurosci. 2013 Oct 9;33(41):16297-309.

    Insert

    Cerulean linked topaz fluorescent protein
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Cerulean, modified Topaz Fluorescent Protein with…

    Promoter

    Available since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pVRa41_491

    Plasmid

    #49702

    Depositor

    Christopher Voigt

    Article

    Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

    Insert

    ECF41_491
    

    Use

    Synthetic Biology

    Tags

    His6-PreScission

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pT7-lacO

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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