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Showing: 7051 - 7100 of 8680 results

  4. pYFP-NEAT1m_v1-RT

    Plasmid
    #97089
    Purpose
    Encodes a HR repair template for knock-in a YFP expression cassette downstream of the poly(A) signal of human NEAT1_1 isoform. Best used with px330-NEAT1m_v1 vector.
    Depositor
    Archa Fox
    Article
    Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d
    Insert
    NEAT1m_v1-RT (NEAT1 Human)
    Use
    CRISPR
    Tags
    EYFP
    Promoter
    CMV
    Available Since
    July 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    97089-plasmid-map-sequence-id-189689

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  5. PGK+ episome

    Plasmid
    #170041
    Purpose
    PGK+ circuit on an episomal vector. This plasmid encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.
    Depositor
    Francois St-Pierre
    Article
    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:
    Insert
    eGFP
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    PGK-tetO2
    Available Since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    170041-plasmid-map-sequence-id-334558

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  8. pTmpKmH207in

    Plasmid
    #176230
    Purpose
    A template plasmid for amplification of the cI-hok-neo cassette, which contains a truncated H207 promoter of T5 phage. The promoter is directed toward the cI gene.
    Depositor
    Sergey Sineoky
    Article
    Bubnov et al Nucleic Acids Res. 2022 Aug 26;50(15):89
    Insert
    cI of phage lambda, hok of the R1 plasmid, neo, truncated PH207 promoter of T5 phage
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available Since
    Nov. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    176230-plasmid-map-sequence-id-347005

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  9. pTmpKmH207out

    Plasmid
    #176231
    Purpose
    A template plasmid for amplification of the cI-hok-neo cassette, which contains a truncated H207 promoter of T5 phage. The promoter is directed outward the cI gene.
    Depositor
    Sergey Sineoky
    Article
    Bubnov et al Nucleic Acids Res. 2022 Aug 26;50(15):89
    Insert
    cI of phage lambda, hok of the R1 plasmid, neo, truncated PH207 promoter of T5 phage
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available Since
    Nov. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    176231-plasmid-map-sequence-id-347006

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  10. pTmpKmA1O4in

    Plasmid
    #176232
    Purpose
    A template plasmid for amplification of the cI-hok-neo cassette, which contains A1O4 promoter. The promoter is directed toward the cI gene.
    Depositor
    Sergey Sineoky
    Article
    Bubnov et al Nucleic Acids Res. 2022 Aug 26;50(15):89
    Insert
    cI of phage lambda, hok of the R1 plasmid, neo, hybrid PA1O4 promoter
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available Since
    Nov. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    176232-plasmid-map-sequence-id-347007

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  11. pTmpKmA1O4out

    Plasmid
    #176233
    Purpose
    A template plasmid for amplification of the cI-hok-neo cassette, which contains LacI-repressible A1O4 promoter. The promoter is directed outward the cI gene.
    Depositor
    Sergey Sineoky
    Article
    Bubnov et al Nucleic Acids Res. 2022 Aug 26;50(15):89
    Insert
    cI of phage lambda, hok of the R1 plasmid, neo, hybrid PA1O4 promoter
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available Since
    Nov. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    176233-plasmid-map-sequence-id-347008

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  14. CMV+ episome

    Plasmid
    #169742
    Purpose
    CMV+ circuit on an episomal vector. This plasmid encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.
    Depositor
    Francois St-Pierre
    Article
    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:
    Insert
    eGFP
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    pCMV-tetO2
    Available Since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    169742-plasmid-map-sequence-id-334220

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  17. pYFAC-CH3

    Plasmid
    #168979
    Purpose
    AMA1 based expression in filamentous fungi pYFAC-riboB carrying PalcA-T1-PalcS/M-T2-PaldA expression cassette ; unique restriction site: PacI for PalcA, NotI for PalcS, AsiSI for PalcM, AscI for PaldA
    Depositor
    Yit Heng Chooi
    Article
    Hu et al Chem Sci. 2018 Nov 22;10(5):1457-1465. d
    Type
    Empty backbone
    Use
    Synthetic Biology; Heterologous expression in asp…
    Available Since
    Dec. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    168979-plasmid-map-sequence-id-350204

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  24. c3GIC9n

    Plasmid
    #62192
    Purpose
    col1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9n (D10A variant) expression. Contains NsiI cloning site for U6-sgRNA cassettes
    Depositor
    Lukas Dow
    Article
    Dow et al Nat Biotechnol. 2015 Apr;33(4):390-4. do
    Insert
    humanized S. Pyogenes Cas9 (D10A)
    Use
    CRISPR
    Tags
    HA
    Mutation
    D10A substitution (Cas9n)
    Promoter
    TRE3G
    Available Since
    Aug. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    62192-plasmid-map-sequence-id-156364

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  25. pEF005-sgRNA-shuffle-in

    Plasmid
    #104441
    Purpose
    Provide and shuffle a cassette of AtU6:sgRNA-transRNA into SM-destination vectors (pRW006 and pRW004) with golden gate cloning strategy. Work together with pEF004.
    Depositor
    Detlef Weigel
    Article
    Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10
    Insert
    sgRNA-transRNA transcription by U6 promoter
    Use
    CRISPR
    Expression
    Bacterial and Plant
    Available Since
    Jan. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    104441-plasmid-map-sequence-id-201145

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  26. pAGK112

    Plasmid
    #228127
    Purpose
    Express node 1531 retron with split retron ncRNA and gRNA to edit (SNIP + PAM recode) HEK4 gene in human cells
    Depositor
    Seth Shipman
    Article
    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103
    Insert
    Retron node 1531 split retron ncRNA and gRNA to edit HEK4 gene (SNP + PAM recode) in human cells
    Expression
    Mammalian
    Promoter
    CAG for RT, U6/H1 for ncRNA/gRNA
    Available Since
    Dec. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    228127-plasmid-map-sequence-id-455412

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  27. DNA-CARtcr-IRESpuro

    Plasmid
    #89346
    Purpose
    2nd generation lentiviral vector which expresses SNAP tagged TCRbeta subunit upstream of a IRESpuro cassette
    Depositor
    Ron Vale
    Article
    Taylor et al Cell. 2017 Mar 23;169(1):108-119.e20. do
    Insert
    TCR beta gene (Jurkat allele) fused at the N terminus to the SNAP tag
    Use
    Lentiviral
    Tags
    SNAPf tag
    Expression
    Mammalian
    Promoter
    SFFV
    Available Since
    June 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    89346-plasmid-map-sequence-id-176306

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  31. pOCC292

    Plasmid
    #118881
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal mRuby3, and C-terminal HIS6 tag, cleavable with 3C
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-NotI-ccdB-AscI-mRuby3-3C-HIS6-stop-HindIII cassette
    Tags
    mRuby3, HIS6, cleavable with 3C protease
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118881-plasmid-map-sequence-id-232308

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  35. pOCC291

    Plasmid
    #118867
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and N-terminal mRuby3
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-3C-mRuby3-NotI-ccdB-AscI-stop-HindIII cassette
    Tags
    HIS6, cleavable with 3C protease, mRuby3
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118867-plasmid-map-sequence-id-232275

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  36. pOCC72

    Plasmid
    #118866
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and N-terminal monomeric GFP, cleavable with TEV
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-3C-mGFP-TEV-NotI-ccdB-AscI-stop-HindIII cassette
    Tags
    HIS6, cleavable with 3C protease, mGFP, cleavable…
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118866-plasmid-map-sequence-id-232323

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  40. pOCC28

    Plasmid
    #118873
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP tag, cleavable with 3C, and C-terminal HIS6 tag, cleavable with 3C
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-MBP-3C--NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
    Tags
    HIS6, cleavable with 3C protease and MBP, cleavab…
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118873-plasmid-map-sequence-id-232344

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  41. pT2SCb

    Plasmid
    #59385
    Purpose
    Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a modified chloramphenicol resistance gene.
    Depositor
    Shelley Copley
    Article
    Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.
    Insert
    TT-ISceI-cat2
    Use
    Template
    Mutation
    The 3’-end of the chloramphenicol resistance gene…
    Available Since
    Sept. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    59385-plasmid-map-sequence-id-92126

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  44. pOCC61

    Plasmid
    #118877
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal monomeric GFP and C-terminal HIS6 tag, cleavable with 3C
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-NotI-ccdB-AscI-mGFP-3C-HIS6-stop-HindIII cassette
    Tags
    mGFP, HIS6, cleavable with 3C protease
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118877-plasmid-map-sequence-id-232346

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  47. sgRNA3_GAL4UAS-Luciferase reporter

    Plasmid
    #64159
    Purpose
    Photoactivatable transcription system. Lentiviral expression of sgRNA3 to target GAL4UAS-luciferase. Also contains a CMV-puro-t2A-mCherry expression cassette
    Depositor
    Moritoshi Sato
    Article
    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi
    Insert
    sgRNA3 for GAL4UAS-Luciferase reporter
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    April 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    64159-plasmid-map-sequence-id-107444

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Showing: 7051 - 7100 of 8680 results