We narrowed to 4,074 results for: p2a

1. pFETCh_TGIF2

   Plasmid

   #72373

   Purpose

   Donor vector for 3' FLAG tag of human TGIF2

   Depositor

   Richard M. Myers, Eric Mendenhall

   Insert

   TGIF2 Homology Arms (TGIF2 Human)
   

   Use

   CRISPR

   Tags

   3XFLAG-P2A-NeoR

   Expression

   Mutation

   Promoter

   Available since

   Feb. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFETCh_ZNF146

   Plasmid

   #72359

   Purpose

   Donor vector for 3' FLAG tag of human ZNF146

   Depositor

   Richard M. Myers, Eric Mendenhall

   Insert

   ZNF146 Homology Arms (ZNF146 Human)
   

   Use

   CRISPR

   Tags

   3XFLAG-P2A-NeoR

   Expression

   Mutation

   Promoter

   Available since

   Feb. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFETCh_PBX2

   Plasmid

   #72356

   Purpose

   Donor vector for 3' FLAG tag of human PBX2

   Depositor

   Richard M. Myers, Eric Mendenhall

   Insert

   PBX2 (PBX2 Human)
   

   Use

   CRISPR

   Tags

   3XFLAG-P2A-NeoR

   Expression

   Mutation

   Promoter

   Available since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. lenti-EF1a-dCas9-VPR-Puro

   Plasmid

   #99373

   Purpose

   3rd generation lenti vector encoding dCas9 (S. pyogenes) fused with VP64-p65-Rta (VPR) and 2A puromycin resistance marker; EF1a-dCas9-VPR-P2A-Puro-WPRE)

   Depositor

   Kristen Brennand

   Article

   Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

   Insert

   (Sp)dCas9-VPR-P2A-Puro
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. Villinpromoter-blue-FlpOERT2

   Plasmid

   #67278

   Purpose

   4hydroxytamoxifen inducible FlpO recombinase controlled by intestine specific promoter (Villin). FlpO is linked to mTQ2 -a blue fluorescent protein. The gene cassette is in a SB transposon context

   Depositor

   Jannik Elverløv-Jakobsen

   Article

   Callesen et al Mol Oncol. 2017 Nov;11(11):1616-1629. do

   Insert

   Villin-mTQ2-P2A-FlpO-ERT2
   

   Use

   Tags

   mTQ2, linked to FlpO through an P2A ribosomal ski…

   Expression

   Mammalian

   Mutation

   Promoter

   Villin

   Available since

   July 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCRIS-PITChv2-dTAG-Puro (BRD4)

   Plasmid

   #91796

   Purpose

   PITCh dTAG donor vector for FKBP_F36V-2xHA-P2A-Puro knock-in into the C-terminus of the human BRD4 locus.

   Depositor

   James Bradner, Behnam Nabet

   Article

   Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

   Insert

   FKBP_F36V-2xHA-P2A-Puro
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Phenylalanine 36 to valine in FKBP12

   Promoter

   Promotorless

   Available since

   March 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCRIS-PITChv2-Puro-dTAG (BRD4)

   Plasmid

   #91793

   Purpose

   PITCh dTAG donor vector for Puro-P2A-2xHA-FKBP_F36V knock-in into the N-terminus of the human BRD4 locus.

   Depositor

   James Bradner, Behnam Nabet

   Article

   Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

   Insert

   Puro-P2A-2xHA-FKBP_F36V
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Phenylalanine 36 to valine in FKBP12

   Promoter

   Promotorless

   Available since

   March 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCRIS-PITChv2-dTAG-BSD (BRD4)

   Plasmid

   #91795

   Purpose

   PITCh dTAG donor vector for FKBP_F36V-2xHA-P2A-BSD knock-in into the C-terminus of the human BRD4 locus.

   Depositor

   James Bradner, Behnam Nabet

   Article

   Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

   Insert

   FKBP_F36V-2xHA-P2A-BSD
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Phenylalanine 36 to valine in FKBP12

   Promoter

   Promotorless

   Available since

   March 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCRIS-PITChv2-BSD-dTAG (BRD4)

   Plasmid

   #91792

   Purpose

   PITCh dTAG donor vector for BSD-P2A-2xHA-FKBP_F36V knock-in into the N-terminus of the human BRD4 locus.

   Depositor

   James Bradner, Behnam Nabet

   Article

   Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

   Insert

   BSD-P2A-2xHA-FKBP_F36V
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Phenylalanine 36 to valine in FKBP12

   Promoter

   Promoterless

   Available since

   March 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAVS1-iPE-Plus

    Plasmid

    #214062

    Purpose

    inducible PE-Plus system for controllable prime editing; this plasmid is used to insert PE-Plus prime editor (PEmax-P2A-hP53DD-IRES-MLH1dn) in one allele of AAVS1 locus

    Depositor

    Ting Zhou

    Article

    Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

    Insert

    PE-Plus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE-tight

    Available since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-nEF-Coff/Fon-ChRmine-oScarlet

    Plasmid

    #137160

    Purpose

    Intersectional viral expression of ChRmine-p2a-oScarlet in cells expressing Flp AND NOT Cre

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Coff/Fon-ChRmine-p2a-oScarlet
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    oScarlet E95D

    Promoter

    nEF

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-nEF-Con/Fon-ChRmine-oScarlet

    Plasmid

    #137159

    Purpose

    Intersectional viral expression of ChRmine-p2a-oScarlet in cells expressing both Cre AND Flp

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Con/Fon-ChRmine-p2a-oScarlet
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    oScarlet E95D

    Promoter

    nEF

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-nEF-Con/Foff 2.0-ChRmine-oScarlet

    Plasmid

    #137161

    Purpose

    Intersectional viral expression of ChRmine-p2a-oScarlet in cells expressing Cre AND NOT Flp

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Con/Foff 2.0-ChRmine-p2a-oScarlet
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    oScarlet E95D

    Promoter

    nEF

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-PE2-p53DD

    Plasmid

    #196582

    Purpose

    Mammalian expression of SpCas9 prime editor 2 with P2A-p53DD

    Depositor

    Ting Zhou

    Article

    Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

    Insert

    PE2-P2A-mp53DD
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Δ40-903

    Promoter

    CMV

    Available since

    March 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPC1000

    Plasmid

    #174829

    Purpose

    Lentiviral Repair-seq vector containing CRISPRi sgRNA and SaPE2 prime edit site

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Inserts

    CRISPRi SpCas9 sgRNA cassette
    PuroR-P2A-BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Detailed in manuscript

    Promoter

    EF1a and mouse U6

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLP-PIGA2 (pLP300)

    Plasmid

    #168462

    Purpose

    Big-IN Landing Pad plasmid encoding pEF1a-driven PuroR-P2A-hmPIGA-P2A-mScarlet expression cassette flanked by heterotypic LoxM/LoxP sites and BsaI homology arms cloning sites.

    Depositor

    Matthew Maurano

    Article

    Brosh et al Proc Natl Acad Sci U S A. 2021 Mar 9;118

    Type

    Empty backbone

    Use

    Cre/Lox, Mouse Targeting, and Synthetic Biology ;…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. Exp-pcDNA3.2delCMV(EF1α-tTA/TetO-mCh-Rs1)

    Plasmid

    #26797

    Depositor

    Edward Hsiao

    Article

    Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

    Insert

    EF1a-tTA/TetO-mCherry-P2A-Rs1 (HTR4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Human 5HT4B receptor with D100A mutation, generat…

    Promoter

    Available since

    Feb. 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. nCas9-UGI control for Target-AID (pRZ1047)

    Plasmid

    #131299

    Purpose

    CAG promoter expression plasmid for NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-NLS-UGI-P2A-EGFP (Target-AID without pmCDA1).

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

    Insert

    NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-NLS-UGI-P2A-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1(+)_Nav1.6WT-HA

    Plasmid

    #239762

    Purpose

    Expresses mouse Nav1.6WT with a C-terminal HA tag in mammalian cells.

    Depositor

    Ivana Nikić-Spiegel

    Article

    Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

    Insert

    Scn8a (Scn8a Mouse)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1(+)_Nav1.6(K1425TAG)-HA

    Plasmid

    #239763

    Purpose

    Expresses mouse Nav1.6 with a TAG codon at position 1425 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.

    Depositor

    Ivana Nikić-Spiegel

    Article

    Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

    Insert

    Scn8a (Scn8a Mouse)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    K1425TAG

    Promoter

    CMV

    Available since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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