We narrowed to 11,993 results for: pcdna

1. pcDNA3.1(+)Gαi2-91-NLuc

   Plasmid

   #207149

   Purpose

   Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI2 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

   Depositor

   Tom Van Loy, Dominique Schols

   Article

   Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

   Insert

   GNAI2 (GNAI2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NLuc inserted at position L(91)Q

   Promoter

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1(−) mCER-Prx2

   Plasmid

   #158224

   Purpose

   Genetically encoded HomoFRET-based probe to monitor peroxiredoxin2 (Prx2) oligomerization (for transient transfection in mammalian cells)

   Depositor

   Tobias Dick

   Article

   Pastor-Flores et al Proc Natl Acad Sci U S A. 2020 Jun 29. p

   Insert

   mCER-Prx2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pY012 (pcDNA3.1-hCMtCpf1)

   Plasmid

   #69984

   Purpose

   Expresses humanized CMtCpf1

   Depositor

   Feng Zhang

   Article

   Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

   Insert

   hCMtCpf1
   

   Use

   CRISPR

   Tags

   NLS-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5-FRT-TO-Nsp12-HF

   Plasmid

   #157711

   Purpose

   mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp12 under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   Nsp12-HF (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   His8-Flag

   Expression

   Mammalian

   Mutation

   codon optimized

   Promoter

   Available Since

   Aug. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pcDNA3 HA Sam68 G178E

   Plasmid

   #17689

   Depositor

   David Shalloway

   Article

   Lin et al J Biol Chem. 1997 Oct 24. 272(43):27274-

   Insert

   Sam68 (Khdrbs1 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   G178E

   Promoter

   Available Since

   April 7, 2008

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA-Pom121 mApple

   Plasmid

   #187674

   Purpose

   In vivo visualization of NPC protein Pom121

   Depositor

   Mary Beckerle

   Insert

   Pom121 (Pom121 Mouse)
   

   Use

   Tags

   mApple

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV

   Available Since

   Sept. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. GLI1_pcDNA6.2/EmGFP-Bsd

   Plasmid

   #176968

   Purpose

   Mammalian expression vector encoding GLI1 and EmGFP-Bsd

   Depositor

   Roger Reeves

   Article

   Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

   Insert

   GLI1 (GLI1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-C-terminal CyPet

   Plasmid

   #128026

   Purpose

   Mammalian expression vector with C terminal CyPet tag

   Depositor

   Oskar Laur

   Type

   Empty backbone

   Use

   Tags

   CyPet

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. Kohinoor 2.0/pcDNA3

   Plasmid

   #182315

   Purpose

   Positively reversibly photoswitchable fluorescent protein

   Depositor

   Takeharu Nagai

   Article

   Wazawa et al Microscopy (Oxf). 2021 Aug 9;70(4):340-3

   Insert

   Kohinoor 2.0
   

   Use

   Tags

   No tag

   Expression

   Mammalian

   Mutation

   Promoter

   T7 promoter

   Available Since

   May 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-CARMA2sh-FLAG

    Plasmid

    #97071

    Purpose

    Expresses CARMA2sh/CARD14 isoform 3 in mammalian cells

    Depositor

    Pasquale Vito

    Article

    Scudiero et al J Cell Physiol. 2011 Dec;226(12):3121-31

    Insert

    CARD14 isoform 3 (CARD14 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3/mArf3(T31N)-HA

    Plasmid

    #79416

    Purpose

    Expresses C-terminally HA-tagged Arf3(T31N) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Kondo et al Cell Struct Funct. 2012;37(2):141-54. Ep

    Insert

    Arf3 (Arf3 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed Threonine 31 to Asparagine

    Promoter

    Available Since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3/mArf3(Q71L)-HA

    Plasmid

    #79415

    Purpose

    Expresses C-terminally HA-tagged Arf3(Q71L) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Kondo et al Cell Struct Funct. 2012;37(2):141-54. Ep

    Insert

    Arf3 (Arf3 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed Glutamine 71 to Leucine

    Promoter

    Available Since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDNA4.TO-ORF61-2xCSTREP

    Plasmid

    #136221

    Purpose

    Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF61

    Depositor

    Britt Glaunsinger

    Article

    Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

    Insert

    ORF61
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDNA5/FRT/TO_HaloTag9_T2A_EGFP

    Plasmid

    #169326

    Purpose

    Expression of HaloTag9 in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    HaloTag9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Promoter

    Available Since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-mCherry-LKB1(KD)

    Plasmid

    #84640

    Purpose

    mCherry-tagged, LKB1(K78I); kinase-dead mutant

    Depositor

    Jin Zhang

    Article

    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

    Insert

    mCherry-LBK1(K78I) (STK11 Human, Synthetic)
    

    Use

    Tags

    6xHis, T7 tag (gene 10 leader), Xpress (TM) tag, …

    Expression

    Mammalian

    Mutation

    Lysine 78 mutated to Isoleucine; kinase dead

    Promoter

    CMV

    Available Since

    May 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. HA-GFP1-10-actin-pcDNA4-TO

    Plasmid

    #118370

    Purpose

    Bimolecular Fluorescence Complimentation (BiFC) assay. Mammalian expression vector to generate stable, inducible cell line expressing N-terminally tagged HA-GFP1-10-actin.

    Depositor

    Maria Vartiainen

    Article

    Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

    Insert

    actin beta (ACTB Human)
    

    Use

    Tags

    HA-GFP1-10

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TO

    Available Since

    May 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA-HA-hTRAF3 1-448

    Plasmid

    #44033

    Depositor

    Shao-Cong Sun

    Article

    Liao et al J Biol Chem. 2004 Jun 18;279(25):26243-5

    Insert

    TNF receptor-associated factor 3 (TRAF3 Human)
    

    Use

    Tags

    1xHA

    Expression

    Mammalian

    Mutation

    Contains amino acids 1-448; V274D and S393G

    Promoter

    Available Since

    April 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. mcherry Gamma 9 ---pcDNA3.1

    Plasmid

    #64203

    Purpose

    G protein Gamma cone(9) subunit tagged with mcherry on the N terminus

    Depositor

    Narasimhan Gautam

    Article

    Karunarathne et al Proc Natl Acad Sci U S A. 2013 Apr 23;11

    Insert

    G protein Gamma 9 (GNGT2 Human)
    

    Use

    Tags

    6xHis and mcherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pF153 pcDNA3.1(+)SOD1G37R

    Plasmid

    #26399

    Depositor

    Elizabeth Fisher

    Article

    Stevens et al PLoS One. 2010 . 5(3):e9541.

    Insert

    SOD1 (SOD1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G37R

    Promoter

    Available Since

    Nov. 23, 2010

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA3-Rs4084-RBD-8h

    Plasmid

    #161825

    Purpose

    Mammalian expression plasmid for RBD of bat coronavirus isolate Rs4084

    Depositor

    Erik Procko

    Article

    Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

    Insert

    Receptor-binding domain (RBD) of spike protein S
    

    Use

    Tags

    GSG linker and 8his tag and HA leader sequence

    Expression

    Mammalian

    Mutation

    Human codon optimized RBD (a.a. T321-D519)

    Promoter

    CMV

    Available Since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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