We narrowed to 17,207 results for: puro

1. N114 nLV EF-1a-Gal4-VP16-PGK-Puro

   Plasmid

   #44014

   Depositor

   Jerry Crabtree

   Article

   Hathaway et al Cell. 2012 Jun 22;149(7):1447-60. doi: 1

   Insert

   Gal4/DBD (1-147)
   

   Use

   Lentiviral

   Tags

   VP16/Activation/Domain

   Expression

   Mutation

   Promoter

   EF-1a

   Available since

   June 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. PiggyBac-TetO-Lmx1a-t2a-Pitx3-puro

   Plasmid

   #176484

   Purpose

   TetO-Lmx1a-t2a-Pitx3 in a PiggyBac vector with puromycin resistance

   Depositor

   Marius Wernig

   Article

   Ng et al Stem Cell Reports. 2021 Jul 13;16(7):176

   Insert

   Lmx1A-T2A-Pitx3 (PITX3, LMX1A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TetO

   Available since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLVX-EF1a-EGFP-TA MAO-IRES-Puromycin

   Plasmid

   #133025

   Purpose

   Expresses EGFP-tagged tail anchor sequence of monoamine oxidase A

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   Monoamine oxidase type A (MAOA Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-488

   Promoter

   Human elongation factor 1 alpha

   Available since

   Feb. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. tetO-GFPnls-mPGK-rtTA-IRES-Puro

   Plasmid

   #69545

   Purpose

   Tet-On system expressing GFP tagged with 2x nuclear localization signals.

   Depositor

   Charles Gersbach

   Insert

   eGFP
   

   Use

   Lentiviral

   Tags

   2x nuclear localization sequence and T2A "sk…

   Expression

   Mutation

   Promoter

   Available since

   April 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBabe-Puro-IKBalpha-mut (super repressor)

   Plasmid

   #15291

   Depositor

   William Hahn

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Insert

   inhibitor of Kappa B alpha (NFKBIA Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   S32A/S36A: “super-repressor” allele (resistant to…

   Promoter

   Available since

   July 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLVX-EF1a-EGFP-VAMP5-IRES-Puromycin

   Plasmid

   #134662

   Purpose

   Expresses EGFP-tagged plasma membrane marker

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   vesicle-associated membrane protein 5 (VAMP5 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLVX-shRNA2-zsGreen-PGK-puro_scramble shRNA

   Plasmid

   #197991

   Purpose

   For the stable knockdown experiments using shRNA, scramble sequence was inserted as the control.

   Depositor

   Bo Wang

   Article

   Lu et al Commun Biol. 2023 Feb 17;6(1):189. doi:

   Insert

   scramble shRNA (control)
   

   Use

   Lentiviral

   Tags

   zsGreen1

   Expression

   Mammalian

   Mutation

   15q11.2 deletion

   Promoter

   Available since

   Nov. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. FLAG-LoxP-PURO-LoxP-HaloTERT WT HR Donor

   Plasmid

   #86843

   Purpose

   HR Donor for N-terminal FLAG-HaloTag-TERT fusion in human cells. Includes Puro selection marker flanked by LoxP sites.

   Depositor

   Thomas Cech

   Article

   Schmidt et al Cell. 2016 Aug 25;166(5):1188-1197.e9. d

   Insert

   FLAG-HaloTag-TERT Exon 1 and part of Exon 2 (TERT Human)
   

   Use

   Tags

   FLAG-HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   Endogenous TERT promoter

   Available since

   March 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEpicVector3mC_Puro-T2A-mCherry-VSVg-StrepTagII-FLAG

   Plasmid

   #162101

   Purpose

   mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

   Depositor

   Julien Sage

   Article

   Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

   Insert

   Puromycin resistant; mCherry linked to VSVg-StrepTagII-FLAG
   

   Use

   Lentiviral

   Tags

   VSVg-StrepTagII-FLAG

   Expression

   Mutation

   WT

   Promoter

   EF1A

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEpicVector3mC_Puro-T2A-mCherry-VSVg-ProtC-AU1

    Plasmid

    #162105

    Purpose

    mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

    Depositor

    Julien Sage

    Article

    Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

    Insert

    Puromycin resistant; mCherry linked to VSVg-ProtC-AU1
    

    Use

    Lentiviral

    Tags

    VSVg-ProtC-AU1

    Expression

    Mutation

    WT

    Promoter

    EF1A

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEpicVector3mC_Puro-T2A-mCherry-StrepTagII-FLAG-AU1

    Plasmid

    #162114

    Purpose

    mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

    Depositor

    Julien Sage

    Article

    Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

    Insert

    Puromycin resistant; mCherry linked to StrepTagII-FLAG-AU1
    

    Use

    Lentiviral

    Tags

    StrepTagII-FLAG-AU1

    Expression

    Mutation

    WT

    Promoter

    EF1A

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEpicVector3G_Puro-T2A-EGFP-VSVg-StrepTagII-ProtC

    Plasmid

    #162079

    Purpose

    EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

    Depositor

    Julien Sage

    Article

    Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

    Insert

    Puromycin resistant; EGFP linked to VSVg-StrepTagII-ProtC
    

    Use

    Lentiviral

    Tags

    VSVg-StrepTagII-ProtC

    Expression

    Mutation

    WT

    Promoter

    EF1A

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLVX-EF1alpha-nCoV2019-N-meGFP-IRES-Puro

    Plasmid

    #185451

    Purpose

    Expresses SARS-CoV-2 nucleocapsid protein with a c-terminal monomeric EGFP in mammalian cells

    Depositor

    Ahmet Yildiz

    Article

    Jack et al PLoS Biol. 2021 Oct 11;19(10):e3001425.

    Insert

    Nucleocapsid (N SARS-CoV-2)
    

    Use

    Lentiviral

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV-EF1a-IRES-Puro GFP-3xFLAG

    Plasmid

    #201636

    Purpose

    Over-expression of 3xFLAG-tagged GFP

    Depositor

    Alexis Jourdain, Vamsi Mootha

    Article

    Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. WT1-2A-eGFP PGK PuroR Donor Plasmid

    Plasmid

    #82333

    Purpose

    WT1 targeting donor

    Depositor

    Sean Palecek

    Article

    Bao et al Nat Biomed Eng. 2016;1. pii: 0003. doi:

    Inserts

    WT1 5' Arm (WT1 Human)
    WT1 3' Arm (WT1 )
    

    Use

    CRISPR and TALEN

    Tags

    2A-eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-C-Myc-DDK-IRES-Puro HEXIM1

    Plasmid

    #200726

    Purpose

    Lentiviral expression of Flag-Tagged human HEXIM1

    Depositor

    Claudio Alarcón

    Insert

    HEXIM1 (HEXIM1 Human)
    

    Use

    Lentiviral

    Tags

    Myc-DDK from backbone plasmid

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV_hU6-sgRNA_hUbC-dSaCas9-KRAB-T2A-PuroR

    Plasmid

    #162334

    Purpose

    Lentiviral expression of dSaCas9-KRAB and a sgRNA with puromycin resistance

    Depositor

    Charles Gersbach

    Article

    Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

    Insert

    dSaCas9-KRAB
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hUbC

    Available since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLVX-EF1a-EGFP-RAB3C-IRES-Puromycin

    Plasmid

    #134857

    Purpose

    Expresses EGFP-tagged synaptic vesicle marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    Ras-related protein 3C
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLVX-EF1a-Calr-KDEL-IRES-Puromycin

    Plasmid

    #134664

    Purpose

    Expresses EGFP-tagged endoplasmic reticulum marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    calreticulin (CALR Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    deleted amino acids 18-413

    Promoter

    EF1a

    Available since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Tol2 8xSTAR-mNeonGreen-NLS. PGK-puro

    Plasmid

    #136262

    Purpose

    Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

    Depositor

    Hugo Snippert

    Article

    Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

    Insert

    stem cell Ascl2 reporter, 8 repeats
    

    Use

    Tol2 transposase

    Tags

    mNeonGreen-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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