We narrowed to 6,950 results for: ust

1. psiCHECK-1-miR-99a-I-type

   Plasmid

   #215888

   Purpose

   This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

   Depositor

   Kumiko Ui-Tei

   Article

   Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

   Insert

   three tandem repeats of seed-matched (SM) sequence of miR-99a-I-type (MIR99A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3puro-mGFP-FXR1a-Ccswap

   Plasmid

   #225460

   Purpose

   Express EGFP-fusion protein of FXR1 isoform a (CCswap)

   Depositor

   Christine Mayr

   Article

   Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

   Insert

   FXR1 isoform a (FXR1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   CCswap (swapped positions of CC2 and CC1)

   Promoter

   CMV

   Available since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3puro-mGFP-FXR1a-CC1-CC1

   Plasmid

   #225458

   Purpose

   Express EGFP-fusion protein of FXR1 isoform a (CC1-CC1)

   Depositor

   Christine Mayr

   Article

   Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

   Insert

   FXR1 isoform a (FXR1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   CC2 replaced with a second copy of CC1

   Promoter

   CMV

   Available since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3puro-mGFP-FXR1-N1-KH1mut (T236D H237D)

   Plasmid

   #225453

   Purpose

   Express mEGFP-fusion protein of FXR1 isoform a (T236D H237D)

   Depositor

   Christine Mayr

   Article

   Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

   Insert

   FXR1 isoform a (FXR1 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   T236D H237D

   Promoter

   CMV

   Available since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pU6-tevopreq1-FXR1-G266E

   Plasmid

   #225482

   Purpose

   Express epegRNA for FXR1

   Depositor

   Christine Mayr

   Article

   Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

   Insert

   epegRNA for editing FXR1-G266E (FXR1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3puro-mGFP-FXR1a-CC2mut (V361P)

   Plasmid

   #225456

   Purpose

   Express mEGFP-fusion protein of FXR1 isoform a (V361P)

   Depositor

   Christine Mayr

   Article

   Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

   Insert

   FXR1 isoform a (FXR1 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   V361P

   Promoter

   CMV

   Available since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3puro-mGFP-FMR1iso1-CC2mut (V427P)

   Plasmid

   #225466

   Purpose

   Express mEGFP-fusion protein of FMR1 isoform 1 (V427P)

   Depositor

   Christine Mayr

   Article

   Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

   Insert

   FMR1 isoform 1 (FMR1 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   V427P

   Promoter

   CMV

   Available since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3puro-mGFP-FXR1a-CC2-CC2

   Plasmid

   #225459

   Purpose

   Express mEGFP-fusion protein of FXR1 isoform a (CC2-CC2)

   Depositor

   Christine Mayr

   Article

   Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

   Insert

   FXR1 isoform a (FXR1 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   CC1 replaced with a second copy of CC2

   Promoter

   CMV

   Available since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. LsgRNA-FXR1-KH1-gRNA

   Plasmid

   #225483

   Purpose

   Express gRNA for FXR1

   Depositor

   Christine Mayr

   Article

   Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

   Insert

   gRNA for editing FXR1-KH1 (FXR1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. BPK1520-FXR1-N202S-gRNA

    Plasmid

    #225481

    Purpose

    Express gRNA for FXR1

    Depositor

    Christine Mayr

    Article

    Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

    Insert

    gRNA for editing FXR1-N202S (FXR1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Oct. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3puro-EGFP-GAPDH-Tudor-R rich

    Plasmid

    #225478

    Purpose

    Express mEGFP-GAPDH-Tudor-R fusion

    Depositor

    Christine Mayr

    Article

    Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

    Insert

    GAPDH fused with Tudor and R-rich regions of TDRD3 (GAPDH Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDM097

    Plasmid

    #216819

    Purpose

    Aspergillus nidulans codon-adjusted mStayGold fluorescent protein, includes linker for N-terminal or internal tagging.

    Depositor

    Ken Sawin

    Article

    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

    Insert

    mStayGold
    

    Use

    Tags

    FLAG-(SGGS)x2-XTEN16-(GGGGS)x3 and c4-(GGGGS)x2-X…

    Expression

    Bacterial

    Mutation

    Aspergillus nidulans codon-adjusted

    Promoter

    Available since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Lenti-Nova1-GFP

    Plasmid

    #217033

    Purpose

    Overexpression of Nova in DRG neurons promotes the "mature" splicing pattern observed in CNS neurons

    Depositor

    Chaolin Zhang

    Article

    Weyn-Vanhentenryck et al Nat Commun. 2018 Jun 6;9(1):2189. doi: 1

    Insert

    Nova1 (Nova1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET SUMO-syt7 (136-403) L361C

    Plasmid

    #213622

    Purpose

    Bacterial expression of SUMO-tagged mouse syt7 (136-403) L361C

    Depositor

    Edwin Chapman

    Article

    Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

    Insert

    syt7 (136-403) L361C (Syt7 Mouse)
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    C260A C275A L361C

    Promoter

    Available since

    March 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET SUMO-cPLA2 C2 domain (Y96C)

    Plasmid

    #213633

    Purpose

    Bacterial expression of SUMO-tagged human cPLA2 C2 domain (Y96C)

    Depositor

    Edwin Chapman

    Article

    Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

    Insert

    cPLA2 C2 domain (Y96C) (PLA2G4A Human)
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Y96C, C139A, C141S

    Promoter

    Available since

    March 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCF702

    Plasmid

    #209650

    Purpose

    Suicide plasmid carrying a neomycin resistance marker flanked by homologous regions of the Mth60-fimbria encoding operon of Methanothermobacter thermautotrophicus

    Depositor

    Bastian Molitor

    Article

    Fink et al Appl Environ Microbiol. 2023 Jul 26;89(7

    Inserts

    Downstream homologous region
    thermostable neomycin phosphotransferase gene
    Upstream homologous region
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV_EF1a_DIO_HA/FLAG_muMASS_LF

    Plasmid

    #213394

    Purpose

    Loss of function variant of uMASS_1 opioid sensor. AAV virus production for Cre dependent expression.

    Depositor

    Andre Berndt

    Article

    Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

    Insert

    uMASS_LF
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV_EF1a_DIO_HA/FLAG_muMASS

    Plasmid

    #213393

    Purpose

    uMASS_1 opioid sensor. AAV Virus production for Cre dependent expression

    Depositor

    Andre Berndt

    Article

    Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

    Insert

    uMASS_1
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV_hsyn_HA_Flag-uMASS_LF

    Plasmid

    #209761

    Purpose

    AAV virus production for neuonal expression of uMASS (loss-of-function) under hSyn promoter

    Depositor

    Andre Berndt

    Article

    Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

    Insert

    uMASS_LF
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-hsyn-dMASS_LF_WPRE

    Plasmid

    #209766

    Purpose

    AAV virus production for neuronal expression of dMASS_LF (loss-of-function) under hSyn promoter

    Depositor

    Andre Berndt

    Article

    Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

    Insert

    dMASS_LF
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge