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Plasmid#45797PurposeFluorescent reporter for calcium signalingDepositorInsertTN-XXL
UseTagsECFP and YFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
mIFP24-C1
Plasmid#54820PurposeLocalization: C1 Cloning Vector, Excitation: 683, Emission: 704DepositorTypeEmpty backboneUseTagsmIFP24ExpressionMammalianMutationPromoterAvailable sinceJune 20, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
MTK4_001
Plasmid#123810PurposeEncodes Bovine Growth Hormone polyadenylation signal as a Type 4 part to be used in the MTK systemDepositorInsertBgh pA
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS2+/N-SNAPf
Plasmid#184415Purposevector backbone to clone insert downstream of SNAPf tag (SNAPf tag codon optimized for Xenopus), for mRNA synthesisDepositorTypeEmpty backboneUseFor mrna synthesisTagsSNAPf-tagExpressionMutationCodon optimized for expression in Xenopus laevis …PromoterAvailable sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2+/C-SNAPf
Plasmid#184416Purposevector backbone to clone insert upstream of SNAPf tag (SNAPf tag codon optimized for Xenopus), for mRNA synthesisDepositorTypeEmpty backboneUseFor mrna synthesisTagsSNAPf-tagExpressionMutationCodon optimized for expression in Xenopus laevis …PromoterAvailable sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
MTK234_032
Plasmid#123995PurposeEncodes the spCas9 sgRNA1 hAAVS1 (site 1) gRNA expression cassette as a type 234 part to be used in the MTK systemDepositorInsertsgRNA1 -hAAVS1
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141D-peg
Plasmid#141081PurposeGateway compatible prime editing gRNA empty backbone vectorDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJune 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR_DMS3g24
Plasmid#89244PurposePlasmid encodes a synthetic CRISPR containing 7 copies of a single spacer, which targets a region (gene 24) in the genome of bacteriophage DMS3DepositorInsertCRISPR
UseTagsExpressionMutationPromoterAvailable sinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFRT/FLAG/HA-DEST EIF2C4
Plasmid#19890DepositorInsertEukaryotic translation initiation factor 2C, 4 (AGO4 Human)
UseTagsFLAG/HAExpressionMammalianMutationPromoterAvailable sinceDec. 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGAN213
Plasmid#129270PurposeControl for measuring background levels of yTRAP reporter proteinDepositorInsertmNeonGreen
UseTagsExpressionYeastMutationPromotermin pCYC1Available sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
SNV-gfp-IRES-MCS
Plasmid#53122PurposeRetrovirus vector backbone that co-expresses GFP and a gene of interest, driven by the Spleen Necrosis Virus promoter; for use in chicken cellsDepositorTypeEmpty backboneUseRetroviral; For expression in chicken cellsTagsEGFP-IRESExpressionMutationPromoterSNV (spleen necrosis virus)Available sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
MTK234_033
Plasmid#123996PurposeEncodes the spCas9 sgRNA1 hAAVS1 (site 2) gRNA expression cassette as a type 234 part to be used in the MTK systemDepositorInsertsgRNA2 -hAAVS1
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK234_034
Plasmid#123997PurposeEncodes the spCas9 sgRNA1 hAAVS1 (site 3) gRNA expression cassette as a type 234 part to be used in the MTK systemDepositorInsertsgRNA3 -hAAVS1
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK0_031
Plasmid#123970PurposeEncodes the BxBI-hygromycin-mRuby2PEST landing pad as a type 0 part to be used in the MTK systemDepositorInsertBxBI-hygro-mRuby2PEST landing
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR-v2-FE-PuroR
Plasmid#186746PurposeModified lentiCRISPRv2 3rd generation lentiviral backbone (Addgene Cat# 52961) containing an FE hairpinDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
PL452-hGH-polyA-PGK-neoR
Plasmid#191681PurposeDonor plasmid backbone PGK-neoRDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationWTPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFH113
Plasmid#128210Purposeimproved sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU3 promoter module (pFH31)DepositorInsertimproved sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU3 promoter module (pFH31)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
EFS eGFP hPGK BSD
Plasmid#188520PurposeBackbone for CRISPR interference (CRISPRi) isogenic PAM testingDepositorTypeEmpty backboneUseLentiviralTagseGFPExpressionMammalianMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-hPDI
Plasmid#60555Purposeenhance solubility and correct disulfide bond-formation of fused protein expressed in E.coliDepositorTypeEmpty backboneUseTagsHis6-hPDIExpressionBacterialMutationPromoterAvailable sinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB326
Plasmid#68654PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsTagRFPExpressionPlantMutationPromoterNo PromoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only