We narrowed to 3,071 results for: psin

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K365-iLid

Plasmid

#188456

Purpose

Component of optically-induced motor dimerization system

Depositor

Zvonimir Dogic

Article

Lemma et al PNAS Nexus. 2023 Apr 12;2(5):pgad130. do

Insert

Kinesin 1-365 (Khc Fly)

Tags

6xHis and iLid

Expression

Bacterial

Mutation

Kinesin amino acids 1-365

Promoter

T7

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pEB1N-mApple-LZ-LOV2

Plasmid

#197418

Purpose

Mammalian expression of the mApple-tagged N-terminal half of photo-inactivated π-EB1

Depositor

Torsten Wittmann

Article

Dema et al Curr Biol. 2022 Mar 14;32(5):1197-1205.e

Insert

MAPRE1 (MAPRE1 Human)

Tags

A. sativa phototropin 1 LOV2 domain, GCN4 leucine…

Expression

Mammalian

Mutation

EB1 aa 1-185; resistant to EB1 shRNA#3 (Addgene #…

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pOTTC1663 - pAAV SYN1 mGas6-Myc-DDK

Plasmid

#202540

Purpose

An adeno-associated viral vector expressing murine Gas6 fused Myc and DDK epitopes from a synapsin promoter

Depositor

Christopher Richie

Article

Owlett et al J Neuroinflammation. 2022 Feb 7;19(1):38

Insert

Gas6 (Gas6 Mouse)

Use

AAV

Tags

Myc-DDK

Promoter

SYN1

Available Since

June 20, 2023

Availability

Academic Institutions and Nonprofits only

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fru-T2A-CsChrimson-tdTomato HDR plasmid

Plasmid

#141100

Purpose

Plasmid for CRISPR-generated knock-in line that expresses optogenetic activator CsChrimson-tdTomato by knocking-in fragment into the coding sequence of the endogenous Aedes aegypti fru gene

Depositor

Leslie Vosshall

Article

Basrur et al Elife. 2020 Dec 7;9. pii: 63982. doi: 10

Insert

fru-left-HDR-arm; T2A-CsChrimson-tdTomato-SV40; 3xP3-EYFP-SV40; fru-right-HDR-arm

Use

Donor template

Available Since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKD5-ChR2-YFP

Plasmid

#178722

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-OFF) of ChR2-YFP in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

ChR2-YFP

Use

AAV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKD6-ChR2-YFP

Plasmid

#178723

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-OFF / FLP-ON) of ChR2-YFP in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

ChR2-YFP

Use

AAV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKS4-ChR2-YFP

Plasmid

#178724

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of ChR2-YFP in neurons under the control of the hSyn1 promoter (Kugler et al 2003)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

ChR2-YFP

Use

AAV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKS6-ChR2-YFP

Plasmid

#178726

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-OFF / FLP-ON) of ChR2-YFP in neurons under the control of the hSyn1 promoter (Kugler et al 2003)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

ChR2-YFP

Use

AAV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pB80-KIF1A(1-365)-GCN4-GFP-SSPB(micro)

Plasmid

#174632

Purpose

Highly processive KIF1A for optogenetic heterodimerization to iLID via SSPB(micro)

Depositor

Lukas Kapitein

Article

Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

KIF1A(1-365)-GCN4-GFP-SSPB(micro) (Kif1a Mouse, Synthetic)

Tags

GFP-SSPB(micro)

Expression

Mammalian

Mutation

mmKIF1A(aa1-365): Pro202Ala; EGFP: Met1Del; SSPB:…

Promoter

Chicken beta-actin

Available Since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pNIA-CEN-FLAG-LANS1-Set2

Plasmid

#122002

Purpose

FLAG-LANS-Set2: FLAG-tagged LANS1-Set2 for light control of Set2 localization in yeast

Depositor

Brian Kuhlman

Article

Lerner et al Genome Res. 2020 Nov;30(11):1605-1617. d

Insert

histone methyltransferase SET2 (SET2 Budding Yeast)

Use

Synthetic Biology

Tags

FLAG

Expression

Yeast

Mutation

Set2 point mutations K538G, K539S, R549G, K550S, …

Promoter

ADH1

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pHR-HOXD13(DEdel)-IDR-mCherry-CRY2-NLS

Plasmid

#145273

Purpose

Mammalian Expression of Nuclear Opto HOXD13(DEdel) IDR with SV40 NLS

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXD13(D/E del)-IDR (HOXD13 Human)

Expression

Mammalian

Mutation

D/E deleted

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-HOXD13(-7A)-IDR-mCherry-CRY2-NLS

Plasmid

#145271

Purpose

Mammalian Expression of Nuclear Opto HOXD13(-7A) IDR with SV40 NLS

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXD13(-7A)-IDR (HOXD13 Human)

Expression

Mammalian

Mutation

A-repeat deleted

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-HOXA13(+7A)-IDR-mCherry-CRY2-NLS

Plasmid

#145266

Purpose

Mammalian Expression of Nuclear Opto HOXA13(+7A) IDR with SV40 NLS

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXA13(+7A)-IDR (HOXA13 Human)

Expression

Mammalian

Mutation

A-repeat expanded

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-HOXA13-IDR-mCherry-CRY2-NLS

Plasmid

#145265

Purpose

Mammalian Expression of Nuclear Opto HOXA13 IDR with SV40 NLS

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXA13wt-IDR (HOXA13 Human)

Expression

Mammalian

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pLuc-Optop38i-lsm

Plasmid

#89747

Purpose

Expression of light-regulated p38 inhibitor, firefly luciferase-fused, lit-state mutant photosensor (AsLov2Ja.I539E), inhibitor MK3BD3-13F

Depositor

Michael Courtney

Article

Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

Insert

Optop38i lit-state mutant

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Mutation

I539E mutation of AsLov2Jalpha

Promoter

CMV

Available Since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pLuc-OptoJNKi-lsm

Plasmid

#89746

Purpose

Expression of light-regulated JNK inhibitor, firefly luciferase-fused, lit-state mutant photosensor (AsLov2Ja.I539E), inhibitor JIP11

Depositor

Michael Courtney

Article

Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

Insert

OptoJNKi lit-state mutant

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Mutation

I539E mutation of AsLov2Jalpha

Promoter

CMV

Available Since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pLuc-OptoJNKi-dsm

Plasmid

#89745

Purpose

Expression of light-regulated JNK inhibitor, firefly luciferase-fused, dark-state mutant photosensor (AsLov2Ja.C450A), inhibitor JIP11

Depositor

Michael Courtney

Article

Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

Insert

OptoJNKi dark-state mutant

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Mutation

C450A mutation of AsLov2Jalpha

Promoter

CMV

Available Since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

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AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE

Plasmid

#51509

Purpose

Can be used to generate AAV virus that will express cytoplasmic tdTomato and presynaptic (synaptophysin-fused) EGFP in the presence of Cre in neurons from the synapsin promoter

Depositor

Hongkui Zeng

Article

Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

Has Service

AAV1

Insert

tdTomato-T2A-SypEGFP

Use

AAV

Promoter

phSyn1

Available Since

May 2, 2014

Availability

Academic Institutions and Nonprofits only

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Flag_HsIRE1a_deltaP29_D408_pBabePuro

Plasmid

#58422

Purpose

retroviral pBabe puro vector encoding N-terminally FLAG tagged mutant human IRE1a deleted from amino acids P28 to D408

Depositor

David Ron

Article

Volmer et al Proc Natl Acad Sci U S A. 2013 Mar 19;11

Insert

IRE1a (ERN1 Human)

Use

Retroviral

Tags

FLAG and preprotrypsin signal sequence

Expression

Mammalian

Mutation

Deleted amino acids P29 to D408

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

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M23: pHR-SFFVp-FUSN::mCherry::SspB

Plasmid

#122148

Purpose

N terminal domain of FUS (1-214) tagged with SspB and mCherry.

Depositor

Clifford Brangwynne

Article

Bracha et al Cell. 2018 Nov 29;175(6):1467-1480.e13.

Insert

FUS (FUS Human)

Use

Lentiviral

Tags

mCherry-sspB

Expression

Mammalian

Mutation

Contains only amino acids 1-214

Promoter

SFFV

Available Since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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